Parkinson’s disease protein DJ-1 regulates ATP synthase protein components to increase neuronal process outgrowth

Objective. To screen the susceptibility genes in Chinese pedigrees with early-onset familial Parkinson's disease (FPD).Methods. Fifty-one genomic DNA samples extracted from two Chinese pedigrees with FPD, the alpha-synuclein genes (SNCA), the leucine-rich repeat kinase 2(LRRK2), PINK1(PTEN-induced putative kinase 1), PARK7(Protein DJ1), PARK2(Parkinson juvenile disease protein 2), the glucocerebrosidase (GBA), and ATP(Ezrin-binding protein PACE-1), were sequenced by the use of polymerase chain reaction (PCR) technique. The gene dose of SNCA was checked.Results. There were only two missense mutations observed, respectively, at exon 5 of LRRK2 and exon 10 of PARK2, and both were enrolled in SNPs.Conclusion. No meaningful mutations could be detected, and other susceptibility genes should be detected in FDP patients in China.

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Inside Cover: Autoproteolytic Fragments Are Intermediates in the Oligomerization/Aggregation of the Parkinson's Disease Protein Alpha-Synuclein as Revealed by Ion Mobility Mass Spectrometry (ChemBioChem 18/2011)

ChemBioChem ◽

10.1002/cbic.201190086 ◽

2011 ◽

Vol 12 (18) ◽

pp. 2706-2706

Author(s):

Camelia Vlad ◽

Kathrin Lindner ◽

Christiaan Karreman ◽

Stefan Schildknecht ◽

Marcel Leist ◽

...

Keyword(s):

Mass Spectrometry ◽

Parkinson’S Disease ◽

Parkinson's Disease ◽

Ion Mobility ◽

Alpha Synuclein ◽

Ion Mobility Mass Spectrometry ◽

Disease Protein

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Expression of the Parkinson's disease protein DJ-1 during the differentiation of neural stem cells

Brain Research ◽

10.1016/j.brainres.2012.05.022 ◽

2012 ◽

Vol 1468 ◽

pp. 84-93 ◽

Cited By ~ 5

Author(s):

Shen Li ◽

Yi Sun ◽

Xin Zhao ◽

Xiao-Ping Pu

Keyword(s):

Parkinson’S Disease ◽

Stem Cells ◽

Parkinson's Disease ◽

Neural Stem Cells ◽

Disease Protein

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Crystallizing the Parkinson’s Disease Protein LRRK2 Under Microgravity Conditions

10.1101/259655 ◽

2018 ◽

Cited By ~ 1

Author(s):

Sebastian Mathea ◽

Marco Baptista ◽

Paul Reichert ◽

April Spinale ◽

Jian Wu ◽

...

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

3D Structure ◽

Space Station ◽

Rational Drug Design ◽

Crystal Formation ◽

Gene Coding ◽

Rational Drug ◽

Disease Protein ◽

Microgravity Conditions

AbstractMutations in the gene coding for leucine-rich repeat kinase 2 (LRRK2) are a considerable cause for Parkinson’s disease (PD). However, the high- resolution 3D structure of the protein is still lacking. This structure will not only help to understand PD etiology but will also enable rational drug design. We have established a reliable method to produce LRRK2 crystals for the first time. However, the limited resolution of the diffraction data prevented structure determination using crystallographic methods. Herein we describe our efforts to improve the crystal quality by crystallizing under microgravity conditions aboard the International Space Station (ISS). Our method features diffusive sample mixing in capillaries and controlled crystal formation by transporting the samples in a frozen state. The crystallisation was successfully repeated under microgravity conditions. However, comparison of earth-grown and microgravity-grown LRRK2 crystals did not reveal any differences in diffraction quality. Here we present the established protocol and our experience adapting crystallization condition to the requirements necessary for successful crystallization of large and sensitive biomolecules under microgravity.

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