scholarly journals Characterising side chains in large proteins by protonless 13C-detected NMR spectroscopy

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Ruth B. Pritchard ◽  
D. Flemming Hansen
Keyword(s):  
Nmr Spectroscopy ◽  
Side Chains ◽  
Large Proteins

Comprehensive and Cost-Effective NMR Spectroscopy of Methyl Groups in Large Proteins

2010 ◽  
Vol 132 (9) ◽  
pp. 2952-2960 ◽  
Author(s):  
Renee Otten ◽  
Byron Chu ◽  
Karla D. Krewulak ◽  
Hans J. Vogel ◽  
Frans A. A. Mulder
Keyword(s):  
Nmr Spectroscopy ◽  
Cost Effective ◽  
Methyl Groups ◽  
Large Proteins

2D and 3D NMR spectroscopy employing carbon-13/carbon-13 magnetization transfer by isotropic mixing. Spin system identification in large proteins

1990 ◽  
Vol 112 (2) ◽  
pp. 886-888 ◽  
Author(s):  
Stephen W. Fesik ◽  
Hugh L. Eaton ◽  
Edward T. Olejniczak ◽  
Erik R. P. Zuiderweg ◽  
Lawrence P. McIntosh ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
System Identification ◽  
Spin System ◽  
Magnetization Transfer ◽  
3D Nmr ◽  
Large Proteins ◽  
Isotropic Mixing ◽  
2D And 3D

scholarly journals Unravelling Main- and Side-Chain Motions in Polymers with NMR Spectroscopy and Relaxometry: The Case of Polyvinyl Butyral

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2686
Author(s):  
Lucia Calucci ◽  
Silvia Pizzanelli ◽  
Alessandro Mandoli ◽  
Artur Birczyński ◽  
Zdzisław T. Lalowicz ◽  
...  
Keyword(s):  
Glass Transition ◽  
Nmr Spectroscopy ◽  
Main Chain ◽  
Amorphous Polymer ◽  
Polyvinyl Butyral ◽  
Side Chains ◽  
Nmr Relaxometry ◽  
Field Cycling ◽  
Nmr Methods ◽  
First Time

Polyvinyl butyral (PVB) is an amorphous polymer employed in many technological applications. In order to highlight the relationships between macroscopic properties and dynamics at a microscopic level, motions of the main-chain and of the propyl side-chains were investigated between Tg − 288 °C and Tg + 55 °C, with Tg indicating the glass transition temperature. To this aim, a combination of solid state Nuclear Magnetic Resonance (NMR) methods was applied to two purposely synthesized PVB isotopomers: one fully protonated and the other perdeuterated on the side-chains. 1H time domain NMR and 1H field cycling NMR relaxometry experiments, performed across and above Tg, revealed that the dynamics of the main-chain corresponds to the α-relaxation associated to the glass transition, which was previously characterized by dielectric spectroscopy. A faster secondary relaxation was observed for the first time and ascribed to side-chains. The geometry and rate of motions of the different groups in the side-chains were characterized below Tg by 2H NMR spectroscopy.

Chemical and 13C NMR Studies of a Rhamnoarabinogalactan from the Leaves of Plantago lanceolata L. var. libor

1994 ◽  
Vol 59 (12) ◽  
pp. 2714-2720 ◽  
Author(s):  
Alžbeta Kardošová ◽  
Peter Capek
Keyword(s):  
Nmr Spectroscopy ◽  
13C Nmr ◽  
Plantago Lanceolata ◽  
Partial Hydrolysis ◽  
Side Chains ◽  
Methylation Analysis ◽  
Nmr Studies ◽  
C Nmr

A rhamnoarabinogalactan isolated from the leaves of Plantago lanceolata L. var. libor has been investigated by methylation analysis, partial hydrolysis, and 13C NMR spectroscopy. The structural conclusions obtained by 13C NMR measurements were consistent with the data from methylation analysis. The polysaccharide has a β-(1→6)-D-galactan core branched on O-3 by side chains of (1→5)-linked α-L-arabinofuranosyl units, some of which bear in position O-5 terminal β-D-galactopyranosyl residues. L-Rhamnose occurs as nonreducing ends.

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