scholarly journals Mechanically rigid supramolecular assemblies formed from an Fmoc-guanine conjugated peptide nucleic acid

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Vasantha Basavalingappa ◽  
Santu Bera ◽  
Bin Xue ◽  
Ido Azuri ◽  
Yiming Tang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Atomic Structure ◽  
Self Assembly ◽  
Peptide Nucleic Acid ◽  
Supramolecular Assemblies ◽  
New Materials ◽  
Structural Patterns ◽  
Mechanical Rigidity ◽  
Hydrogen Bonded

AbstractThe variety and complexity of DNA-based structures make them attractive candidates for nanotechnology, yet insufficient stability and mechanical rigidity, compared to polyamide-based molecules, limit their application. Here, we combine the advantages of polyamide materials and the structural patterns inspired by nucleic-acids to generate a mechanically rigid fluorenylmethyloxycarbonyl (Fmoc)-guanine peptide nucleic acid (PNA) conjugate with diverse morphology and photoluminescent properties. The assembly possesses a unique atomic structure, with each guanine head of one molecule hydrogen bonded to the Fmoc carbonyl tail of another molecule, generating a non-planar cyclic quartet arrangement. This structure exhibits an average stiffness of 69.6 ± 6.8 N m−1 and Young’s modulus of 17.8 ± 2.5 GPa, higher than any previously reported nucleic acid derived structure. This data suggests that the unique cation-free “basket” formed by the Fmoc-G-PNA conjugate can serve as an attractive component for the design of new materials based on PNA self-assembly for nanotechnology applications.

Molecular insights on the dynamic stability of peptide nucleic acid functionalized carbon and boron nitride nanotubes

2021 ◽  
Vol 23 (1) ◽  
pp. 219-228
Author(s):  
Nabanita Saikia ◽  
Mohamed Taha ◽  
Ravindra Pandey
Keyword(s):  
Nucleic Acid ◽  
Boron Nitride ◽  
Nucleic Acids ◽  
Dynamic Stability ◽  
Peptide Nucleic Acid ◽  
Rational Design ◽  
Peptide Nucleic Acids ◽  
Boron Nitride Nanotubes ◽  
Molecular Hybrids ◽  
Single Wall Nanotubes

The rational design of self-assembled nanobio-molecular hybrids of peptide nucleic acids with single-wall nanotubes rely on understanding how biomolecules recognize and mediate intermolecular interactions with the nanomaterial's surface.

scholarly journals Peptide Nucleic Acids: Applications in Biomedical Sciences

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3317
Author(s):  
Eylon Yavin
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Peptide Nucleic Acid ◽  
Peptide Nucleic Acids ◽  
Biomedical Sciences

The DNA mimic, PNA (peptide nucleic acid), has been with us now for almost 3 decades [...]

Synthesis, Characterization, Investigation of Capacitance and Redox Properties of Self-assembled Phe-Phe with Ferrocene Conjugates

2021 ◽  
Author(s):  
Om Shanker Tiwari
Keyword(s):  
Nucleic Acid ◽  
Electrochemical Properties ◽  
Self Assembly ◽  
Peptide Nucleic Acid ◽  
Redox Properties ◽  
Self Assembled ◽  
Acid Unit

The synthesis, nanostructure self-assembly and electrochemical properties of a series of ferrocene conjugated peptides derived from the dipeptide Phe-Phe, nucleoside and the peptide nucleic acid unit which are covalently attached...

Supramolecular Nanodrugs Constructed by Self-Assembly of Peptide Nucleic Acid–Photosensitizer Conjugates for Photodynamic Therapy

2019 ◽  
Vol 3 (1) ◽  
pp. 2-9 ◽  
Author(s):  
Rui Chang ◽  
Emmanouil Nikoloudakis ◽  
Qianli Zou ◽  
Anna Mitraki ◽  
Athanassios G. Coutsolelos ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Nucleic Acid ◽  
Self Assembly ◽  
Peptide Nucleic Acid

Self‐Assembly of Di‐Guanine Peptide Nucleic Acid Amphiphiles into Fractal Patterns

2019 ◽  
Vol 4 (46) ◽  
pp. 13525-13532 ◽  
Author(s):  
Jancy N. Abraham ◽  
Prabhakar Pawar ◽  
Dilna K. Kootteri
Keyword(s):  
Nucleic Acid ◽  
Self Assembly ◽  
Peptide Nucleic Acid ◽  
Fractal Patterns

Self-Assembly of Hybridized Peptide Nucleic Acid Amphiphiles

2014 ◽  
Vol 3 (5) ◽  
pp. 467-471 ◽  
Author(s):  
Li-Han Liu ◽  
Ze-Yong Li ◽  
Lei Rong ◽  
Si-Yong Qin ◽  
Qi Lei ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Self Assembly ◽  
Peptide Nucleic Acid

scholarly journals Peptide Nucleic Acid–Based In Situ Hybridization Assay for Detection of Parvovirus B19 Nucleic Acids

2006 ◽  
Vol 52 (6) ◽  
pp. 973-978 ◽  
Author(s):  
Francesca Bonvicini ◽  
Claudia Filippone ◽  
Elisabetta Manaresi ◽  
Giovanna Angela Gentilomi ◽  
Marialuisa Zerbini ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Peptide Nucleic Acid ◽  
Parvovirus B19 ◽  
Hybridization Assay ◽  
Pcr Assay ◽  
Clinical Specimens ◽  
Rna Molecules

Abstract Background: Peptide nucleic acid (PNA) molecules are known to bind complementary nucleic acid sequences with a much stronger affinity and with more stable binding than DNA or RNA molecules. We chose parvovirus B19, which is diagnosed by detection of nucleic acids by in situ hybridization assay (ISH) and/or PCR, as an experimental model to develop an ISH assay that uses biotinylated PNA probes to detect viral genome in clinical specimens. Methods: We first optimized the PNA-ISH assay on B19-infected and mock-infected UT-7/EpoS1 cells and then tested the assay on archival B19 specimens and on consecutive specimens. All data were compared with data obtained with a standardized DNA-based ISH assay and confirmed by a PCR-ELISA. Results: PNA-ISH detected B19 genome in a higher number of B19-infected UT-7/EpoS1 cells and with a more defined localization of viral nucleic acids than the standardized DNA-ISH assay. Moreover, PNA-ISH was able to detect B19 genome in all positive archival samples, whereas DNA-ISH failed in 5 samples. PNA-ISH detected more positive samples than DNA-ISH when consecutive specimens were analyzed, and a close agreement was found with PCR-ELISA results. Conclusions: The PNA-ISH assay had sensitivity and specificity comparable to a PCR assay and was more practical and quicker to perform than standard hybridization assays. The assay may be a suitable diagnostic test for the detection of viral nucleic acids in clinical specimens.

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