scholarly journals Polydiacetylene-based ultrastrong bioorthogonal Raman probes for targeted live-cell Raman imaging

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Sidan Tian ◽  
Haozheng Li ◽  
Zhong Li ◽  
Huajun Tang ◽  
Mingming Yin ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Raman Imaging ◽  
Live Cell ◽  
Water Soluble ◽  
Imaging Agents ◽  
Great Promise ◽  
High Quality ◽  
Topochemical Polymerization ◽  
Silent Region

AbstractLive-cell Raman imaging based on bioorthogonal Raman probes with distinct signals in the cellular Raman-silent region (1800–2800 cm−1) has attracted great interest in recent years. We report here a class of water-soluble and biocompatible polydiacetylenes with intrinsic ultrastrong alkyne Raman signals that locate in this region for organelle-targeting live-cell Raman imaging. Using a host-guest topochemical polymerization strategy, we have synthesized a water-soluble and functionalizable master polydiacetylene, namely poly(deca-4,6-diynedioic acid) (PDDA), which possesses significantly enhanced (up to ~104 fold) alkyne vibration compared to conventional alkyne Raman probes. In addition, PDDA can be used as a general platform for multi-functional ultrastrong Raman probes. We achieve high quality live-cell stimulated Raman scattering imaging on the basis of modified PDDA. The polydiacetylene-based Raman probes represent ultrastrong intrinsic Raman imaging agents in the Raman-silent region (without any Raman enhancer), and the flexible functionalization of this material holds great promise for its potential diverse applications.

scholarly journals Raman Amplifier Based on Amorphous Silicon Nanoparticles

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
M. A. Ferrara ◽  
I. Rendina ◽  
S. N. Basu ◽  
L. Dal Negro ◽  
L. Sirleto
Keyword(s):  
Raman Scattering ◽  
Amorphous Silicon ◽  
Silicon Nanoparticles ◽  
Stimulated Raman Scattering ◽  
Pump Laser ◽  
Great Promise ◽  
Threshold Power ◽  
Raman Lasers ◽  
Superlattice Structures ◽  
Amorphous Silicon Nanoparticles

The observation of stimulated Raman scattering in amorphous silicon nanoparticles embedded in Si-rich nitride/silicon superlattice structures (SRN/Si-SLs) is reported. Using a 1427 nm continuous-wavelength pump laser, an amplification of Stokes signal up to 0.9 dB/cm at 1540.6 nm and a significant reduction in threshold power of about 40% with respect to silicon are experimentally demonstrated. Our results indicate that amorphous silicon nanoparticles are a great promise for Si-based Raman lasers.

scholarly journals Live-Cell Quantitative Imaging of Proteome Degradation by Stimulated Raman Scattering

2014 ◽  
Vol 53 (22) ◽  
pp. 5596-5599 ◽  
Author(s):  
Yihui Shen ◽  
Fang Xu ◽  
Lu Wei ◽  
Fanghao Hu ◽  
Wei Min
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Quantitative Imaging ◽  
Live Cell ◽  
Stimulated Raman

scholarly journals Stimulated Raman scattering of polymer nanoparticles for multiplexed live-cell imaging

2017 ◽  
Vol 53 (46) ◽  
pp. 6187-6190 ◽  
Author(s):  
Fanghao Hu ◽  
Spencer D. Brucks ◽  
Tristan H. Lambert ◽  
Luis M. Campos ◽  
Wei Min
Keyword(s):  
Raman Scattering ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Stimulated Raman Scattering ◽  
Live Cell ◽  
Polymer Nanoparticles ◽  
Biocompatible Polymer ◽  
Photo Stability ◽  
Stimulated Raman

Biocompatible polymer nanoparticles with vibrational labels incorporated were readily prepared for live-cell multiplexed stimulated Raman scattering imaging with excellent photo-stability.

scholarly journals Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

2014 ◽  
Vol 11 (4) ◽  
pp. 410-412 ◽  
Author(s):  
Lu Wei ◽  
Fanghao Hu ◽  
Yihui Shen ◽  
Zhixing Chen ◽  
Yong Yu ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Stimulated Raman Scattering ◽  
Live Cell ◽  
Small Biomolecules ◽  
Stimulated Raman

Live-Cell Stimulated Raman Scattering Imaging of Alkyne-Tagged Biomolecules

2014 ◽  
Vol 53 (23) ◽  
pp. 5827-5831 ◽  
Author(s):  
Senlian Hong ◽  
Tao Chen ◽  
Yuntao Zhu ◽  
Ang Li ◽  
Yanyi Huang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Live Cell ◽  
Stimulated Raman

scholarly journals Raman Imaging of Nanocarriers for Drug Delivery

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 341 ◽  
Author(s):  
Sally Vanden-Hehir ◽  
William Tipping ◽  
Martin Lee ◽  
Valerie Brunton ◽  
Anna Williams ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Imaging Techniques ◽  
Raman Imaging ◽  
The Body ◽  
Label Free ◽  
Dosing Strategy ◽  
Pharmaceutical Agents

The efficacy of pharmaceutical agents can be greatly improved through nanocarrier delivery. Encapsulation of pharmaceutical agents into a nanocarrier can enhance their bioavailability and biocompatibility, whilst also facilitating targeted drug delivery to specific locations within the body. However, detailed understanding of the in vivo activity of the nanocarrier-drug conjugate is required prior to regulatory approval as a safe and effective treatment strategy. A comprehensive understanding of how nanocarriers travel to, and interact with, the intended target is required in order to optimize the dosing strategy, reduce potential off-target effects, and unwanted toxic effects. Raman spectroscopy has received much interest as a mechanism for label-free, non-invasive imaging of nanocarrier modes of action in vivo. Advanced Raman imaging techniques, including coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), are paving the way for rigorous evaluation of nanocarrier activity at the single-cell level. This review focuses on the development of Raman imaging techniques to study organic nanocarrier delivery in cells and tissues.

scholarly journals Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes

2016 ◽  
Vol 49 (8) ◽  
pp. 1494-1502 ◽  
Author(s):  
Lu Wei ◽  
Fanghao Hu ◽  
Zhixing Chen ◽  
Yihui Shen ◽  
Luyuan Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Stimulated Raman Scattering ◽  
Chemical Imaging ◽  
Live Cell ◽  
Stimulated Raman
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