scholarly journals BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
John Maringa Githaka ◽  
Namita Tripathi ◽  
Raven Kirschenman ◽  
Namrata Patel ◽  
Vrajesh Pandya ◽  
...  
Keyword(s):  
Cell Migration ◽  
Mammary Gland ◽  
Mammary Gland Development ◽  
Focal Adhesions ◽  
Mrna Translation ◽  
Protein Functions ◽  
Mammary Gland Morphogenesis ◽  
Gland Morphogenesis ◽  
Gland Development

AbstractElucidation of non-canonical protein functions can identify novel tissue homeostasis pathways. Herein, we describe a role for the Bcl-2 family member BAD in postnatal mammary gland morphogenesis. In Bad3SA knock-in mice, where BAD cannot undergo phosphorylation at 3 key serine residues, pubertal gland development is delayed due to aberrant tubulogenesis of the ductal epithelium. Proteomic and RPPA analyses identify that BAD regulates focal adhesions and the mRNA translation repressor, 4E-BP1. These results suggest that BAD modulates localized translation that drives focal adhesion maturation and cell motility. Consistent with this, cells within Bad3SA organoids contain unstable protrusions with decreased compartmentalized mRNA translation and focal adhesions, and exhibit reduced cell migration and tubulogenesis. Critically, protrusion stability is rescued by 4E-BP1 depletion. Together our results confirm an unexpected role of BAD in controlling localized translation and cell migration during mammary gland development.

scholarly journals Insights into the role of connexins in mammary gland morphogenesis and function

Reproduction ◽  
2015 ◽  
Vol 149 (6) ◽  
pp. R279-R290 ◽  
Author(s):  
Michael K G Stewart ◽  
Jamie Simek ◽  
Dale W Laird
Keyword(s):  
Mammary Gland ◽  
Gap Junctions ◽  
Normal Tissue ◽  
Mammary Gland Development ◽  
Mammary Gland Morphogenesis ◽  
Gland Morphogenesis ◽  
Cell Death Mechanisms ◽  
And Function ◽  
Gland Development

Gap junctions formed of connexin subunits link adjacent cells by direct intercellular communication that is essential for normal tissue homeostasis in the mammary gland. The mammary gland undergoes immense remodeling and requires exquisite regulation to control the proliferative, differentiating, and cell death mechanisms regulating gland development and function. The generation of novel genetically modified mice with reduced or ablated connexin function within the mammary gland has advanced our understanding of the role of gap junctions during the complex and dynamic process of mammary gland development. These studies have revealed an important stage-specific role for Cx26 (GJA1) and Cx43 (GJB2), while Cx30 (GJB6) and Cx32 (Gjb1) can be eliminated without compromising the gland. Yet, there remain gaps in our understanding of the role of mammary gland gap junctions.

The role of prolactin and growth hormone in mammary gland development

2002 ◽  
Vol 197 (1-2) ◽  
pp. 127-131 ◽  
Author(s):  
Paul A Kelly ◽  
Anne Bachelot ◽  
Cécile Kedzia ◽  
Lothar Hennighausen ◽  
Christopher J Ormandy ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Mammary Gland ◽  
Mammary Gland Development ◽  
Gland Development

Lifelong exposure to n-3 PUFA affects pubertal mammary gland development

2014 ◽  
Vol 39 (6) ◽  
pp. 699-706 ◽  
Author(s):  
Breanne M. Anderson ◽  
Mira B. MacLennan ◽  
Lyn M. Hillyer ◽  
David W.L. Ma
Keyword(s):  
Breast Cancer ◽  
Mammary Gland ◽  
Breast Cancer Risk ◽  
Cancer Risk ◽  
Dietary Fat ◽  
Mammary Gland Development ◽  
Morphological Development ◽  
Terminal End Buds ◽  
Gland Development

There is growing evidence that early developmental periods may importantly influence future breast cancer risk. Also, there is great interest in the role of dietary fat in breast cancer risk, but the role of dietary fat during pubertal mammary gland development remains poorly understood. This study investigated the effect of n-3 polyunsaturated fatty acids (PUFA) using complementary dietary and genetic approaches to examine the effect of lifelong exposure of n-3 PUFA or n-6 PUFA (control) on mammary gland development and fatty acid composition. n-3 PUFA from both diet and genetics were enriched in mammary glands as early as 3 weeks of age. Parameters related to mammary gland development, including number of terminal end buds (TEB), percent coverage of ductal tree, and infiltration of TEB, were influenced by n-3 PUFA at 3 and 4 weeks of age. Overall, findings suggest that n-3 PUFA incorporation into the mammary gland early in life plays a role in the morphological development of the mammary gland during puberty.

scholarly journals Role of JNK in Mammary Gland Development and Breast Cancer

2011 ◽  
Vol 72 (2) ◽  
pp. 472-481 ◽  
Author(s):  
Cristina Cellurale ◽  
Nomeda Girnius ◽  
Feng Jiang ◽  
Julie Cavanagh-Kyros ◽  
Shaolei Lu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mammary Gland ◽  
Mammary Gland Development ◽  
Gland Development
Sign in / Sign up

Export Citation Format

Share Document