scholarly journals One-step construction of circularized nanodiscs using SpyCatcher-SpyTag

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shanwen Zhang ◽  
Qian Ren ◽  
Scott J. Novick ◽  
Timothy S. Strutzenberg ◽  
Patrick R. Griffin ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Synaptic Transmission ◽  
Lipid Bilayers ◽  
Structural Biology ◽  
Viral Entry ◽  
Vesicle Fusion ◽  
Structural Studies ◽  
Lipid Interactions ◽  
Complex Membrane ◽  
One Step

AbstractCircularized nandiscs (cNDs) exhibit superb monodispersity and have the potential to transform functional and structural studies of membrane proteins. In particular, cNDs can stabilize large patches of lipid bilayers for the reconstitution of complex membrane biochemical reactions, enabling the capture of crucial intermediates involved in synaptic transmission and viral entry. However, previous methods for building cNDs require multiple steps and suffer from low yields. We herein introduce a simple, one-step approach to ease the construction of cNDs using the SpyCatcher-SpyTag technology. This approach increases the yield of cNDs by over 10-fold and is able to rapidly generates cNDs with diameters ranging from 11 to over 100 nm. We demonstrate the utility of these cNDs for mechanistic interrogations of vesicle fusion and protein-lipid interactions that are unattainable using small nanodiscs. Together, the remarkable performance of SpyCatcher-SpyTag in nanodisc circularization paves the way for the use of cNDs in membrane biochemistry and structural biology.

scholarly journals Raman Scattering: From Structural Biology to Medical Applications

Crystals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 38 ◽  
Author(s):  
Alexey V. Vlasov ◽  
Nina L. Maliar ◽  
Sergey V. Bazhenov ◽  
Evelina I. Nikelshparg ◽  
Nadezda A. Brazhe ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Membrane Proteins ◽  
Drug Design ◽  
Raman Scattering ◽  
Structural Biology ◽  
Surface Enhanced Raman Spectroscopy ◽  
Water Soluble ◽  
Diagnostic Tools ◽  
Medical Applications ◽  
Structural Studies

This is a review of relevant Raman spectroscopy (RS) techniques and their use in structural biology, biophysics, cells, and tissues imaging towards development of various medical diagnostic tools, drug design, and other medical applications. Classical and contemporary structural studies of different water-soluble and membrane proteins, DNA, RNA, and their interactions and behavior in different systems were analyzed in terms of applicability of RS techniques and their complementarity to other corresponding methods. We show that RS is a powerful method that links the fundamental structural biology and its medical applications in cancer, cardiovascular, neurodegenerative, atherosclerotic, and other diseases. In particular, the key roles of RS in modern technologies of structure-based drug design are the detection and imaging of membrane protein microcrystals with the help of coherent anti-Stokes Raman scattering (CARS), which would help to further the development of protein structural crystallography and would result in a number of novel high-resolution structures of membrane proteins—drug targets; and, structural studies of photoactive membrane proteins (rhodopsins, photoreceptors, etc.) for the development of new optogenetic tools. Physical background and biomedical applications of spontaneous, stimulated, resonant, and surface- and tip-enhanced RS are also discussed. All of these techniques have been extensively developed during recent several decades. A number of interesting applications of CARS, resonant, and surface-enhanced Raman spectroscopy methods are also discussed.

scholarly journals Controlled delivery of membrane proteins to artificial lipid bilayers by nystatin–ergosterol modulated vesicle fusion

2006 ◽  
Vol 153 (2) ◽  
pp. 21 ◽  
Author(s):  
M.R.R. de Planque ◽  
G.P. Mendes ◽  
M. Zagnoni ◽  
M.E. Sandison ◽  
K.H. Fisher ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Vesicle Fusion ◽  
Controlled Delivery ◽  
Artificial Lipid Bilayers

3D Crystallization and Structural Studies of Integral Membrane Proteins in Lipidic Cubic phases

2000 ◽  
Vol 56 (s1) ◽  
pp. s83-s83
Author(s):  
P. Nollert ◽  
M. L. Chiu ◽  
M. C. Loewen ◽  
A. Royant ◽  
H. Behrhali ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Structural Studies ◽  
Cubic Phases

scholarly journals Lipid Transporters Beam Signals from Cell Membranes

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 562
Author(s):  
Miliça Ristovski ◽  
Danny Farhat ◽  
Shelly Ellaine M. Bancud ◽  
Jyh-Yeuan Lee
Keyword(s):  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Lipid Composition ◽  
Structural Integrity ◽  
Current Knowledge ◽  
Integral Membrane Proteins ◽  
Cellular Membranes ◽  
Cytoplasmic Proteins ◽  
Lipid Transporters

Lipid composition in cellular membranes plays an important role in maintaining the structural integrity of cells and in regulating cellular signaling that controls functions of both membrane-anchored and cytoplasmic proteins. ATP-dependent ABC and P4-ATPase lipid transporters, two integral membrane proteins, are known to contribute to lipid translocation across the lipid bilayers on the cellular membranes. In this review, we will highlight current knowledge about the role of cholesterol and phospholipids of cellular membranes in regulating cell signaling and how lipid transporters participate this process.

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