BCR affinity differentially regulates colonization of the subepithelial dome and infiltration into germinal centers within Peyer’s patches

A feature of rabbit gut-associated lymphoid tissue is that its structure is more developed than in other animal species. In rabbits it is composed of sacculus rotundus, vermiform appendix and Peyer’s patches. These immune formations contain an organized component of lymphoid tissue – lymphoid nodules (B-cell zone) and interfollicular region (T-cell). Secondary lymphoid nodules with germinal centers presented in them are formed due to antigen stimulation. The caecum of Hyplus rabbits at the age of 30 -, 60 - and 90-days was investigated. Each age group consisted of 5 rabbits. Experimental rabbits are clinically healthy, unvaccinated and untreated against ecto- and endoparasites. Peyer’s patches of the caecum were selected for the study and fixed in 10% of formalin. Subsequently, the specimens stained with hematoxylin-eosin were prepared from the obtained samples. On the 30th day of life, Peyer’s patches in the cecum were detected by gross examination. On the histological level, they had formed interfollicular region and lymphoid nodules. In turn, lymphoid nodules were divided into primary and secondary ones. A well-defined mantle zone and germinal centers were observed in the secondary lymphoid nodules. The regularities of their area indicators increase (mean value, median and interquartile range (IQR)) and their correlation were studied. The most intensive growth of the mantle area and the germinal center was observed from the 30th to the 60th day. The relative area of the mantle zone and the germinal center as part of the secondary lymphoid nodule was determined. Its value did not change during the experimental period.

Download Full-text

Cytokinetic Studies on Tingible Bodies in Germinal Centers of Peyer’s Patches in Mice

Lymphatic Tissue and Germinal Centers in Immune Response - Advances in Experimental Medicine and Biology ◽

10.1007/978-1-4684-3192-6_10 ◽

1969 ◽

pp. 93-100 ◽

Cited By ~ 2

Author(s):

N. Odartchenko ◽

B. Sordat ◽

M. Pavillard ◽

H. Cottier

Keyword(s):

Germinal Centers ◽

Peyer's Patches ◽

Peyer’S Patches

Download Full-text

Decreased Apoptosis in the Ileum and Ileal Peyer's Patches: a Feature after Infection with Rabbit EnteropathogenicEscherichia coli O103

Infection and Immunity ◽

10.1128/iai.69.7.4580-4589.2001 ◽

2001 ◽

Vol 69 (7) ◽

pp. 4580-4589 ◽

Cited By ~ 29

Author(s):

Ursula Heczko ◽

Chris M. Carthy ◽

Bronwyn A. O'Brien ◽

B. Brett Finlay

Keyword(s):

Caspase 3 ◽

Cultured Cells ◽

Apoptotic Cell ◽

Light And Electron Microscopy ◽

Germinal Centers ◽

Peyer's Patches ◽

Infection Model ◽

Peyer’S Patches ◽

Rabbit Ileum ◽

Cell Counts

ABSTRACT Significant changes occur in intestinal epithelial cells after infection with enteropathogenic Escherichia coli (EPEC). However, it is unclear whether this pathogen alters rates of apoptosis. By using a naturally occurring weaned rabbit infection model, we determined physiological levels of apoptosis in rabbit ileum and ileal Peyer's patches (PP) and compared them to those found after infection with adherent rabbit EPEC (REPEC O103). Various REPEC O103 strains were first tested in vitro for characteristic virulence features. Rabbits were then inoculated with the REPEC O103 strains that infected cultured cells the most efficiently. After experimental infection, intestinal samples were examined by light and electron microscopy. Simultaneously, ileal apoptosis was assessed by using terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) and caspase 3 assays and by apoptotic cell counts based on morphology (hematoxylin-and-eosin staining). The highest physiological apoptotic indices were measured in PP germinal centers (median = 14.7%), followed by PP domed villi (8.1%), tips of absorptive villi (3.8%), and ileal crypt regions (0.5%). Severe infection with REPEC O103 resulted in a significant decrease in apoptosis in PP germinal centers (determined by TUNEL assay; P = 0.01), in the tips of ileal absorptive villi (determined by H&E staining;P = 0.04), and in whole ileal cell lysates (determined by caspase 3 assay; P = 0.001). We concluded that REPEC O103 does not promote apoptosis. Furthermore, we cannot rule out the possibility that REPEC O103, in fact, decreases apoptotic levels in the rabbit ileum.

Download Full-text

DEFICIENCY OF IgM

PEDIATRICS ◽

10.1542/peds.47.2.399 ◽

1971 ◽

Vol 47 (2) ◽

pp. 399-404

Author(s):

W. P. Faulk ◽

W. S. Kiyasu ◽

M. D. Cooper ◽

H. H. Fudenberg

Keyword(s):

Lymph Nodes ◽

Plasma Cells ◽

Germinal Centers ◽

Peyer's Patches ◽

Peyer’S Patches ◽

Pseudomonas Infection ◽

Spleen And Lymph Nodes ◽

Genetically Determined

An 8½-month-old infant with absent IgM had recurrent Pseudomonas infections. IgG and IgA, but no IgM-containing plasma cells, were identified in the spleen by immunofluorescence. The spleen and lymph nodes lacked germinal centers, but Peyer's patches and the appendix were normal. The absence of IgM was perhaps genetically determined because the father's serum IgM was also low. This may have predisposed to the Pseudomonas infection, since antibodies to Pseudomonas are predominantly IgM.

Download Full-text

B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer’s Patches

Cell Reports ◽

10.1016/j.celrep.2020.01.032 ◽

2020 ◽

Vol 30 (6) ◽

pp. 1910-1922.e5 ◽

Cited By ~ 2

Author(s):

Adi Biram ◽

Eitan Winter ◽

Alice E. Denton ◽

Irina Zaretsky ◽

Bareket Dassa ◽

...

Keyword(s):

B Cell ◽

Germinal Centers ◽

Peyer's Patches ◽

Peyer’S Patches

Download Full-text

Modulation of lymphocyte subsets in Peyer's patches of mice treated with monoclonal antibody against helper T-cells.

Journal of Histochemistry & Cytochemistry ◽

10.1177/36.4.2964470 ◽

1988 ◽

Vol 36 (4) ◽

pp. 417-423 ◽

Cited By ~ 3

Author(s):

T H Ermak ◽

H J Steger ◽

R L Owen ◽

M F Heyworth

Keyword(s):

Monoclonal Antibody ◽

T Cells ◽

Lymphocyte Subsets ◽

Germinal Centers ◽

Peyer's Patches ◽

Helper T Cells ◽

Size Difference ◽

Peyer’S Patches ◽

Peyer’S Patch ◽

Peyer's Patch

Treatment of mice with anti-L3T4, a monoclonal antibody directed against helper T-cells, impairs clearance of intestinal Giardia muris infection. The present study examined the effect of anti-L3T4 treatment on mouse Peyer's patch cytoarchitecture and on the distribution of T-cell subsets within microenvironments of the follicle. Female BALB/c mice, aged 8 weeks, were given 4-7 weekly injections of either anti-L3T4 (1 mg/wk) or PBS (control group), and Peyer's patches were examined by immunohistochemistry or flow cytometry. In anti-L3T4-treated mice, Peyer's patch follicles (B-cell areas) were about two thirds the size of follicles in controls, and virtually all the size difference occurred in germinal centers. Peyer's patches were depleted of L3T4+ cells, yet the proportion of Thy-1.2+ (all T) cells was not decreased correspondingly, and the distribution of Thy-1.2+ cells in the patches was similar to that in control mice. In anti-L3T4-treated mice, Thy-1.2+ cells consisted of (a) Ly-2+ (cytotoxic/suppressor T) cells, and (b) a population of Thy-1.2+ cells that were neither L3T4+ nor Ly-2+. After treatment, Ly-2+ cells accounted for most of the T-cells in interfollicular areas and were also scattered in follicles, in germinal centers, and below the dome epithelium--in areas where L3T4+ cells predominated in control mice. Thy-1.2+ cells that were L3T4- and Ly-2- were mainly localized below the dome epithelium. These shifts indicate complex interrelationships among different lymphocyte subsets in Peyer's patches.

Download Full-text