scholarly journals Synergistic Effect of BDNF and FGF2 in Efficient Generation of Functional Dopaminergic Neurons from human Mesenchymal Stem Cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Manisha Singh ◽  
Anupama Kakkar ◽  
Rinkey Sharma ◽  
O. P. Kharbanda ◽  
Nitika Monga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synergistic Effect ◽  
Dopaminergic Neurons ◽  
Human Mesenchymal Stem Cells ◽  
Efficient Generation

scholarly journals Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep

Hepatology ◽  
2007 ◽  
Vol 46 (6) ◽  
pp. 1935-1945 ◽  
Author(s):  
Jason Chamberlain ◽  
Takashi Yamagami ◽  
Evan Colletti ◽  
Neil D. Theise ◽  
Jyoti Desai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Human Hepatocytes ◽  
Fetal Sheep ◽  
Efficient Generation

scholarly journals Graphene nanofiber composites for enhanced neuronal differentiation of human mesenchymal stem cells

Nanomedicine ◽  
2021 ◽  
Author(s):  
Sonali Rawat ◽  
Krishan Gopal Jain ◽  
Deepika Gupta ◽  
Pawan Kumar Raghav ◽  
Rituparna Chaudhuri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neuronal Differentiation ◽  
Dopaminergic Neurons ◽  
Human Mesenchymal Stem Cells ◽  
Cost Effective ◽  
Intracellular Ca ◽  
One Step ◽  
Neuronal Arborization ◽  
Temperature Storage

Aim: To differentiate mesenchymal stem cells into functional dopaminergic neurons using an electrospun polycaprolactone (PCL) and graphene (G) nanocomposite. Methods: A one-step approach was used to electrospin the PCL nanocomposite, with varying G concentrations, followed by evaluating their biocompatibility and neuronal differentiation. Results: PCL with exiguous graphene demonstrated an ideal nanotopography with an unprecedented combination of guidance stimuli and substrate cues, aiding the enhanced differentiation of mesenchymal stem cells into dopaminergic neurons. These newly differentiated neurons were seen to exhibit unique neuronal arborization, enhanced intracellular Ca2+ influx and dopamine secretion. Conclusion: Having cost-effective fabrication and room-temperature storage, the PCL-G nanocomposites could pave the way for enhanced neuronal differentiation, thereby opening a new horizon for an array of applications in neural regenerative medicine.

scholarly journals Evaluation of Priming Efficiency of Forskolin in Tissue-Specific Human Mesenchymal Stem Cells into Dopaminergic Neurons: An In Vitro Comparative Study

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2058
Author(s):  
Manisha Singh ◽  
Pardeep Kumar Vaishnav ◽  
Amit Kumar Dinda ◽  
Sujata Mohanty
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Ion ◽  
Dopaminergic Neurons ◽  
Neuronal Cells ◽  
Human Mesenchymal Stem Cells ◽  
Generate Functional ◽  
Efficient Manner ◽  
Protein Levels

Background: Human mesenchymal stem cells (hMSC) can be derived from various tissue sources and differentiated into dopaminergic (DAergic) neurons using various types of inducers. There are several strategies that have been reported to generate functional dopaminergic neuronal cells from hMSCs in the most efficient manner possible. However, this area is still under extensive research. In this study, we aim to compare hMSCs derived from bone marrow (BM), adipose tissue (AD) and dental pulp (DP) to generate functional dopaminergic neurons, using FGF2 and forskolin. Post-differentiation, multiple factors were used to characterize the cells at morphological, morphometric, ultra-structural, mRNA and protein levels for various markers (Nestin, NF, MAP2, Tuj1, TH, DAT, PitX3, Ngn2, Kv4.2, SCN5A). Cells’ functionality was studied by calcium ion imaging, along with the amount of dopamine secreted by the cells in the culture medium. Results: Data analysis revealed that forskolin has comparable effect on BM- and AD-derived MSC (28.43% and 29.46% DAergic neurons, respectively), whereas DP-MSC (42.78 ± 1.248% DAergic neurons) show better outcome in terms of efficient generation of DAergic neuronal cells, expression of neuronal associated markers, dopamine release and calcium ion efflux. Ultra-structural studies by SEM and TEM also revealed a substantial change in both cellular morphology and composition of cellular organelles. It was observed that AD-MSCs showed the best neuronal features, at morphological, gene, and protein levels upon induction with the above-mentioned induction cocktail. Conclusion: It may be concluded that a combination of FGF2 and forskolin yields functionally active dopaminergic neuronal cells in vitro, with highest percentage of the same from AD-MSCs, as compared to that in BM-MSCs and DP-MSCs. The outcomes and comparative evaluation provide a substantial platform for further studies on molecular pathways involved in the process of DAergic neurogenesis in individual cases.

Neuroprotective effects of human mesenchymal stem cells on dopaminergic neurons through anti-inflammatory action

Glia ◽  
2009 ◽  
Vol 57 (1) ◽  
pp. 13-23 ◽  
Author(s):  
You-Joung Kim ◽  
Hyun-Jung Park ◽  
Gwang Lee ◽  
Oh Young Bang ◽  
Young Hwan Ahn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dopaminergic Neurons ◽  
Human Mesenchymal Stem Cells ◽  
Neuroprotective Effects ◽  
Anti Inflammatory ◽  
Inflammatory Action

scholarly journals Synergistic acceleration in the osteogenesis of human mesenchymal stem cells by graphene oxide–calcium phosphate nanocomposites

2014 ◽  
Vol 50 (62) ◽  
pp. 8484-8487 ◽  
Author(s):  
Rameshwar Tatavarty ◽  
Hao Ding ◽  
Guijin Lu ◽  
Robert J. Taylor ◽  
Xiaohong Bi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Calcium Phosphate ◽  
Synergistic Effect ◽  
Human Mesenchymal Stem Cells ◽  
Stem Cell Differentiation ◽  
Calcium Phosphate Nanoparticles

Nanocomposites consisting of oblong ultrathin plate shaped calcium phosphate nanoparticles and graphene oxide microflakes were synthesized and have demonstrated markedly synergistic effect in accelerating stem cell differentiation to osteoblasts.

Sign in / Sign up

Export Citation Format

Share Document