scholarly journals Increased throughput and ultra-high mass resolution in DESI FT-ICR MS imaging through new-generation external data acquisition system and advanced data processing approaches

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Pieter C. Kooijman ◽  
Konstantin O. Nagornov ◽  
Anton N. Kozhinov ◽  
David P. A. Kilgour ◽  
Yury O. Tsybin ◽  
...  
Keyword(s):  
Data Processing ◽  
Data Acquisition ◽  
Data Acquisition System ◽  
High Mass ◽  
External Data ◽  
Ms Imaging ◽  
High Mass Resolution ◽  
New Generation ◽  
Ft Icr Ms ◽  
Ft Icr

scholarly journals Direct Imaging of Plant Metabolites in the Rhizosphere Using Laser Desorption Ionization Ultra-High Resolution Mass Spectrometry

2021 ◽  
Vol 12 ◽  
Author(s):  
Martin Lohse ◽  
Rebecca Haag ◽  
Eva Lippold ◽  
Doris Vetterlein ◽  
Thorsten Reemtsma ◽  
...  
Keyword(s):  
Laser Desorption ◽  
High Mass ◽  
Laser Desorption Ionization ◽  
Plant Metabolites ◽  
Undisturbed Soil ◽  
Desorption Ionization ◽  
High Mass Resolution ◽  
Ft Icr Ms ◽  
Surrounding Soil ◽  
Ft Icr

The interplay of rhizosphere components such as root exudates, microbes, and minerals results in small-scale gradients of organic molecules in the soil around roots. The current methods for the direct chemical imaging of plant metabolites in the rhizosphere often lack molecular information or require labeling with fluorescent tags or isotopes. Here, we present a novel workflow using laser desorption ionization (LDI) combined with mass spectrometric imaging (MSI) to directly analyze plant metabolites in a complex soil matrix. Undisturbed samples of the roots and the surrounding soil of Zea mays L. plants from either field- or laboratory-scale experiments were embedded and cryosectioned to 100 μm thin sections. The target metabolites were detected with a spatial resolution of 25 μm in the root and the surrounding soil based on accurate masses using ultra-high mass resolution laser desorption ionization Fourier-transform ion cyclotron resonance mass spectrometry (LDI-FT-ICR-MS). Using this workflow, we could determine the rhizosphere gradients of a dihexose (e.g., sucrose) and other plant metabolites (e.g., coumaric acid, vanillic acid). The molecular gradients for the dihexose showed a high abundance of this metabolite in the root and a strong depletion of the signal intensity within 150 μm from the root surface. Analyzing several sections from the same undisturbed soil sample allowed us to follow molecular gradients along the root axis. Benefiting from the ultra-high mass resolution, isotopologues of the dihexose could be readily resolved to enable the detection of stable isotope labels on the compound level. Overall, the direct molecular imaging via LDI-FT-ICR-MS allows for the first time a non-targeted or targeted analysis of plant metabolites in undisturbed soil samples, paving the way to study the turnover of root-derived organic carbon in the rhizosphere with high chemical and spatial resolution.

Design and Implement of Data Acquisition System Based on Reconfigurable Computing

2012 ◽  
Vol 546-547 ◽  
pp. 1393-1397
Author(s):  
Zhi Wen Xiong ◽  
Chen Guang Xu ◽  
Hong Zeng
Keyword(s):  
Data Processing ◽  
Data Acquisition ◽  
Reconfigurable Computing ◽  
Rapid Development ◽  
Physical Property ◽  
Data Acquisition System ◽  
Digital System ◽  
Working Environment ◽  
Acquisition System ◽  
Computing Technology

Data acquisition begins with the physical phenomenon or physical property to be measured. Examples of this include temperature, gas pressure, and light intensity, and force, fluid flow, regardless of the type of physical property to be measured. Physical property converted into digital, and then by the computer for storage, processing, display or printing process, the corresponding system is called data acquisition system. With the rapid development of computer technology, data acquisition systems quickly gained popularity. A variety of products based on digital technology have been created. Digital System spread quickly; it’s mainly the following two advantages: the first is the digital processing flexible and convenient; the second is a digital system is very reliable. The main idea of Reconfigurable computing technology [1] is using the FPGA [2][3] allows the system has a dynamically configurable capacity, suitable for harsh environment applications, improve the speed of data processing. By the use of dynamic reconfigurable FPGA devices can be realized on the hardware logic function modification, application of reconfigurable computing technology can improve the speed of data processing. Data acquisition system is widely applied in many fields, and often used the abominable working environment place. The reconfigurable computing technology, can greatly improve the data acquisition system reliability and safety. The paper introduces a kind of multi-channel data acquisition system based on USB bus and FPGA, the factors affecting the performance of system are discussed, and describes how to use reconfigurable computing technology to improve the efficiency of data acquisition system while reduce energy consumption. The system in this paper uses AD's AD9220, ALTERA's EP1C6-8 and IDT's IDT70V24, Cypress’s CY7C68013.

scholarly journals RECONSTRUCTION OF INDOOR MODELS USING POINT CLOUDS GENERATED FROM SINGLE-LENS REFLEX CAMERAS AND DEPTH IMAGES

2015 ◽  
Vol XL-4/W5 ◽  
pp. 99-102
Author(s):  
F. Tsai ◽  
T.-S. Wu ◽  
I.-C. Lee ◽  
H. Chang ◽  
A. Y. S. Su
Keyword(s):  
Data Processing ◽  
Data Acquisition ◽  
Three Dimensional ◽  
Point Clouds ◽  
Data Acquisition System ◽  
Acquisition System ◽  
Indoor Environments ◽  
Single Lens ◽  
Processing Procedure ◽  
Data Processing Procedure

This paper presents a data acquisition system consisting of multiple RGB-D sensors and digital single-lens reflex (DSLR) cameras. A systematic data processing procedure for integrating these two kinds of devices to generate three-dimensional point clouds of indoor environments is also developed and described. In the developed system, DSLR cameras are used to bridge the Kinects and provide a more accurate ray intersection condition, which takes advantage of the higher resolution and image quality of the DSLR cameras. Structure from Motion (SFM) reconstruction is used to link and merge multiple Kinect point clouds and dense point clouds (from DSLR color images) to generate initial integrated point clouds. Then, bundle adjustment is used to resolve the exterior orientation (EO) of all images. Those exterior orientations are used as the initial values to combine these point clouds at each frame into the same coordinate system using Helmert (seven-parameter) transformation. Experimental results demonstrate that the design of the data acquisition system and the data processing procedure can generate dense and fully colored point clouds of indoor environments successfully even in featureless areas. The accuracy of the generated point clouds were evaluated by comparing the widths and heights of identified objects as well as coordinates of pre-set independent check points against in situ measurements. Based on the generated point clouds, complete and accurate three-dimensional models of indoor environments can be constructed effectively.

Distributed real time data processing architecture for the TJ-II data acquisition system

2004 ◽  
Vol 75 (10) ◽  
pp. 4261-4264 ◽  
Author(s):  
M. Ruiz ◽  
E. Barrera ◽  
S. López ◽  
D. Machón ◽  
J. Vega ◽  
...  
Keyword(s):  
Data Processing ◽  
Data Acquisition ◽  
Real Time ◽  
Data Acquisition System ◽  
Acquisition System ◽  
Time Data ◽  
Real Time Data ◽  
Real Time Data Processing ◽  
Processing Architecture

scholarly journals MIDAS-W: a workstation-based incoherent scatter radar data acquisition system

2000 ◽  
Vol 18 (9) ◽  
pp. 1231-1241 ◽  
Author(s):  
J. M. Holt ◽  
P. J. Erickson ◽  
A. M. Gorczyca ◽  
T. Grydeland
Keyword(s):  
Data Processing ◽  
Data Acquisition ◽  
High Speed ◽  
Data Acquisition System ◽  
Acquisition System ◽  
Incoherent Scatter Radar ◽  
Raw Data ◽  
Incoherent Scatter ◽  
Scatter Radar ◽  
Instruments And Techniques

Abstract. The Millstone Hill Incoherent Scatter Data Acquisition System (MIDAS) is based on an abstract model of an incoherent scatter radar. This model is implemented in a hierarchical software system, which serves to isolate hardware and low-level software implementation details from higher levels of the system. Inherent in this is the idea that implementation details can easily be changed in response to technological advances. MIDAS is an evolutionary system, and the MIDAS hardware has, in fact, evolved while the basic software model has remained unchanged. From the earliest days of MIDAS, it was realized that some functions implemented in specialized hardware might eventually be implemented by software in a general-purpose computer. MIDAS-W is the realization of this concept. The core component of MIDAS-W is a Sun Microsystems UltraSparc 10 workstation equipped with an Ultrarad 1280 PCI bus analog to digital (A/D) converter board. In the current implementation, a 2.25 MHz intermediate frequency (IF) is bandpass sampled at 1 µs intervals and these samples are multicast over a high-speed Ethernet which serves as a raw data bus. A second workstation receives the samples, converts them to filtered, decimated, complex baseband samples and computes the lag-profile matrix of the decimated samples. Overall performance is approximately ten times better than the previous MIDAS system, which utilizes a custom digital filtering module and array processor based correlator. A major advantage of MIDAS-W is its flexibility. A portable, single-workstation data acquisition system can be implemented by moving the software receiver and correlator programs to the workstation with the A/D converter. When the data samples are multicast, additional data processing systems, for example for raw data recording, can be implemented simply by adding another workstation with suitable software to the high-speed network. Testing of new data processing software is also greatly simplified, because a workstation with the new software can be added to the network without impacting the production system. MIDAS-W has been operated in parallel with the existing MIDAS-1 system to verify that incoherent scatter measurements by the two systems agree. MIDAS-W has also been used in a high-bandwidth mode to collect data on the November, 1999, Leonid meteor shower.Key words: Electromagnetics (instruments and techniques; signal processing and adaptive antennas) – Ionosphere (instruments and techniques)

Research of Automatic Camshaft Detection and Data Processing Technology

2010 ◽  
Vol 42 ◽  
pp. 404-407
Author(s):  
Li Juan Zhong ◽  
Jing Lin Tong
Keyword(s):  
Data Processing ◽  
Data Acquisition ◽  
Evaluation Method ◽  
Measurement Instrument ◽  
Data Acquisition System ◽  
Processing Method ◽  
Mechanical Measurement ◽  
Detection Technology ◽  
Data Processing Method ◽  
Data Processing Methods

The traditional optical-mechanical measurement instrument as well as manual data processing methods has unable to meet the efficient and accurate detection requirements for camshaft. In this article, the principle of automatic camshaft detection technology and the control system’s structure are introduced. The data acquisition system independent of movement controller and the data processing method of "all the sensitive" and evaluation method are introduced sequent.

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