scholarly journals In-situ ToF-SIMS analyses of deuterium re-distribution in austenitic steel AISI 304L under mechanical load

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Andreas Röhsler ◽  
Oded Sobol ◽  
Hannu Hänninen ◽  
Thomas Böllinghaus
Keyword(s):  
Austenitic Steel ◽  
Mechanical Load ◽  
Aisi 304L ◽  
Tof Sims ◽  
Steel Aisi

Optics-Free Visualization of Proteins in Single Cells by Time of Flight-Secondary Ion Mass Spectrometry Coupled with Genetically Encoded Chemical Tags

2020 ◽  
Author(s):  
Feifei Jia ◽  
Jie Wang ◽  
Yanyan Zhang ◽  
Qun Luo ◽  
Luyu Qi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Single Cells ◽  
Time Of Flight ◽  
E Coli ◽  
Tof Sims ◽  
Ion Mass Spectrometry ◽  
Chemical Tags ◽  
Secondary Ion

<p></p><p><i>In situ</i> visualization of proteins of interest at single cell level is attractive in cell biology, molecular biology and biomedicine, which usually involves photon, electron or X-ray based imaging methods. Herein, we report an optics-free strategy that images a specific protein in single cells by time of flight-secondary ion mass spectrometry (ToF-SIMS) following genetic incorporation of fluorine-containing unnatural amino acids as a chemical tag into the protein via genetic code expansion technique. The method was developed and validated by imaging GFP in E. coli and human HeLa cancer cells, and then utilized to visualize the distribution of chemotaxis protein CheA in E. coli cells and the interaction between high mobility group box 1 protein and cisplatin damaged DNA in HeLa cells. The present work highlights the power of ToF-SIMS imaging combined with genetically encoded chemical tags for <i>in situ </i>visualization of proteins of interest as well as the interactions between proteins and drugs or drug damaged DNA in single cells.</p><p></p>

scholarly journals Influence of Activated Fluxes on the Bead Shape of A-TIG Welds on Carbon and Low-Alloy Steels in Comparison with Stainless Steel AISI 304L

Metals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 530
Author(s):  
Jerzy Niagaj
Keyword(s):  
Stainless Steel ◽  
Penetration Depth ◽  
High Strength ◽  
Tig Welding ◽  
Low Alloy Steels ◽  
Aisi 304L ◽  
A Tig Welding ◽  
Alloy Steels ◽  
Steel Aisi ◽  
Stainless Steel Aisi

The article presents results of comparative A-TIG welding tests involving selected unalloyed and fine-grained steels, as well as high-strength steel WELDOX 1300 and austenitic stainless steel AISI 304L. The tests involved the use of single ingredient activated fluxes (Cr2O3, TiO2, SiO2, Fe2O3, NaF, and AlF3). In cases of carbon and low-alloy steels, the tests revealed that the greatest increase in penetration depth was observed in the steels which had been well deoxidized and purified during their production in steelworks. The tests revealed that among the activated fluxes, the TiO2 and SiO2 oxides always led to an increase in penetration depth during A-TIG welding, regardless of the type and grade of steel. The degree of the aforesaid increase was restricted within the range of 30% to more than 200%.

scholarly journals Comprehensive study of deuterium-induced effects in austenitic stainless steel AISI 304L

2019 ◽  
Vol 44 (23) ◽  
pp. 12228-12238 ◽  
Author(s):  
Andreas Röhsler ◽  
Oded Sobol ◽  
Wolfgang E.S. Unger ◽  
Thomas Böllinghaus
Keyword(s):  
Stainless Steel ◽  
Austenitic Stainless Steel ◽  
Aisi 304L ◽  
Steel Aisi ◽  
Stainless Steel Aisi ◽  
Comprehensive Study
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