scholarly journals Serial optical coherence microscopy for label-free volumetric histopathology

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Eunjung Min ◽  
Sungbea Ban ◽  
Junwon Lee ◽  
Andrey Vavilin ◽  
Songyee Baek ◽  
...  
Keyword(s):  
Large Scale ◽  
Ex Vivo ◽  
Optical Microscope ◽  
Optical Coherence ◽  
Label Free ◽  
Deep Tissue ◽  
Optical Coherence Microscopy ◽  
Volume Imaging ◽  
Vessel Structure ◽  
Microscopic Pathology

AbstractThe observation of histopathology using optical microscope is an essential procedure for examination of tissue biopsies or surgically excised specimens in biological and clinical laboratories. However, slide-based microscopic pathology is not suitable for visualizing the large-scale tissue and native 3D organ structure due to its sampling limitation and shallow imaging depth. Here, we demonstrate serial optical coherence microscopy (SOCM) technique that offers label-free, high-throughput, and large-volume imaging of ex vivo mouse organs. A 3D histopathology of whole mouse brain and kidney including blood vessel structure is reconstructed by deep tissue optical imaging in serial sectioning techniques. Our results demonstrate that SOCM has unique advantages as it can visualize both native 3D structures and quantitative regional volume without introduction of any contrast agents.

scholarly journals 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jun Zhu ◽  
Hercules Rezende Freitas ◽  
Izumi Maezawa ◽  
Lee-way Jin ◽  
Vivek J. Srinivasan
Keyword(s):  
Minimally Invasive ◽  
Mouse Brain ◽  
Numerical Aperture ◽  
Optical Biopsy ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Minimal Invasiveness ◽  
Cortical Regions

AbstractIn vivo, minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge, we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm, where ballistic attenuation in the brain is minimized. Key issues, including detector noise, excess light source noise, chromatic dispersion, and the resolution-speckle tradeoff, are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space, we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex, and some sub-cortical regions. In an Alzheimer’s disease model, we report that findings in superficial and deep cortical layers diverge, highlighting the importance of deep optical biopsy. Compared to other microscopic techniques, our 1700 nm OCM approach achieves a unique combination of intrinsic contrast, minimal invasiveness, and high resolution for deep brain imaging.

Multi-functional optical coherence microscopy for in-vitro and ex-vivo tissue investigation

2021 ◽  
Author(s):  
Yoshiaki Yasuno ◽  
Ibrahim Abd El-Sadek ◽  
Arata Miyazawa ◽  
Larina Tzu-Wei Shen ◽  
Thitiya Seesan ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Optical Coherence ◽  
Optical Coherence Microscopy ◽  
Ex Vivo Tissue

scholarly journals Label-free three-dimensional imaging of Caenorhabditis elegans with visible optical coherence microscopy

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181676 ◽  
Author(s):  
Séverine Coquoz ◽  
Paul J. Marchand ◽  
Arno Bouwens ◽  
Laurent Mouchiroud ◽  
Vincenzo Sorrentino ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Three Dimensional ◽  
Optical Coherence ◽  
Label Free ◽  
Optical Coherence Microscopy ◽  
Three Dimensional Imaging

scholarly journals Integrated Optical Coherence Tomography and Optical Coherence Microscopy Imaging of Ex Vivo Human Renal Tissues

2012 ◽  
Vol 187 (2) ◽  
pp. 691-699 ◽  
Author(s):  
Hsiang-Chieh Lee ◽  
Chao Zhou ◽  
David W. Cohen ◽  
Amy E. Mondelblatt ◽  
Yihong Wang ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Ex Vivo ◽  
Optical Coherence ◽  
Microscopy Imaging ◽  
Optical Coherence Microscopy ◽  
Integrated Optical

scholarly journals Signal-to-background ratio and lateral resolution in deep tissue imaging by optical coherence microscopy in the 1700 nm spectral band

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Masahito Yamanaka ◽  
Naoki Hayakawa ◽  
Norihiko Nishizawa
Keyword(s):  
Spectral Band ◽  
Lateral Resolution ◽  
Tissue Imaging ◽  
Optical Coherence ◽  
Deep Tissue ◽  
Optical Coherence Microscopy ◽  
Background Ratio ◽  
Tissue Phantom ◽  
En Face ◽  
Deep Tissue Imaging

Abstract We quantitatively investigated the image quality in deep tissue imaging with optical coherence microscopy (OCM) in the 1700 nm spectral band, in terms of the signal-to-background ratio (SBR) and lateral resolution. In this work, to demonstrate the benefits of using the 1700 nm spectral band for OCM imaging of brain samples, we compared the imaging quality of OCM en-face images obtained at the same position by using a hybrid 1300 nm/1700 nm spectral domain (SD) OCM system with shared sample and reference arms. By observing a reflective resolution test target through a 1.5 mm-thick tissue phantom, which had a similar scattering coefficient to brain cortex tissue, we confirmed that 1700 nm OCM achieved an SBR about 6-times higher than 1300 nm OCM, although the lateral resolution of the both OCMs was similarly degraded with the increase of the imaging depth. Finally, we also demonstrated high-contrast deep tissue imaging of a mouse brain at a depth up to 1.8 mm by using high-resolution 1700 nm SD-OCM.

scholarly journals Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast

2012 ◽  
Vol 20 (3) ◽  
pp. 2220 ◽  
Author(s):  
Vivek J. Srinivasan ◽  
Harsha Radhakrishnan ◽  
James Y. Jiang ◽  
Scott Barry ◽  
Alex E. Cable
Keyword(s):  
Cerebral Cortex ◽  
Tissue Imaging ◽  
Optical Coherence ◽  
Deep Tissue ◽  
Optical Coherence Microscopy ◽  
Deep Tissue Imaging
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