scholarly journals Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kleopatra Leontidou ◽  
Savvas Genitsaris ◽  
Anastasia Papadopoulou ◽  
Nathalie Kamou ◽  
Irene Bosmali ◽  
...  
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Gene Clusters ◽  
Stress Factors ◽  
Wild Plant ◽  
Whole Genome Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Plant growth promoting rhizobacteria (PGPR) are able to provide cross-protection against multiple stress factors and facilitate growth of their plant symbionts in many ways. The aim of this study was to isolate and characterize rhizobacterial strains under natural conditions, associated with naturally occurring representatives of wild plant species and a local tomato cultivar, growing in differently stressed Mediterranean ecosystems. A total of 85 morphologically different rhizospheric strains were isolated; twenty-five exhibited multiple in vitro PGP-associated traits, including phosphate solubilization, indole-3-acetic acid production, and 1-aminocyclopropane-1-carboxylate deaminase activity. Whole genome analysis was applied to eight selected strains for their PGP potential and assigned seven strains to Gammaproteobacteria, and one to Bacteroidetes. The genomes harboured numerous genes involved in plant growth promotion and stress regulation. They also support the notion that the presence of gene clusters with potential PGP functions is affirmative but not necessary for a strain to promote plant growth under abiotic stress conditions. The selected strains were further tested for their ability to stimulate growth under stress. This initial screening led to the identification of some strains as potential PGPR for increasing crop production in a sustainable manner.

scholarly journals Genomic Mining and Physiological Characterization of new Halophilic Plant Growth Promoting Bacilli

2021 ◽  
Author(s):  
Claudia Petrillo ◽  
Stefany Castaldi ◽  
Mariamichela Lanzilli ◽  
Matteo Selci ◽  
Angelina Cordone ◽  
...  
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Growth Promotion ◽  
Biological Activities ◽  
Gene Clusters ◽  
Plant Growth Promoting Bacteria ◽  
Dual Culture ◽  
Whole Genome Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Massive application of chemical fertilizers and pesticides has been the main strategy used to cope with the rising crop demands in the last decades. The indiscriminate use of chemicals while providing a temporary solution has led to a decrease in crop productivity and an increase in the environmental impact of modern agriculture. A sustainable alternative to the use of chemicals for crop production is the use of microorganisms naturally capable of enhancing plant growth and protecting crops from pests, known as Plant-Growth-Promoting Bacteria (PGPB). The aim of the present study was to isolate and characterize PGPB from salt-pans sand samples able to ameliorate plant fitness. To survive high salinity, salt-tolerant microbes produce a broad range of compounds with heterogeneous biological activities that are potentially beneficial for plant growth. We have isolated and screened in vitro a total of 20 halophilic spore-forming bacteria for phyto-beneficial traits and compared the results with two rhizosphere Bacilli recently isolated from the rhizosphere of the same collection site and recently characterized as potential biocontrol agents. Whole-genome analysis on five selected halophilic strains confirmed the presence of numerous gene clusters with PGP and biocontrol functions and of novel secondary-metabolite biosynthetic genes potentially involved in plant growth promotion and protection. The predicted biocontrol potential was confirmed in dual culture assays against several phytopathogenic fungi and bacteria. Interestingly, the absence of predicted gene clusters with known biocontrol functions in some of the isolates was not predictive of the in vivo results, supporting the need of combining laboratory assays and genome mining in PGPB identification for future applications.

scholarly journals Role of Plant Growth-Promoting Rhizobacteria (PGPR) and Bio-Control Agents (BCAs) in Crop Production

2020 ◽  
Vol 7 (3) ◽  
pp. 144-150
Author(s):  
Sujata Kumari ◽  
◽  
Narender K. Bharat ◽  
Ashok K. Thakur ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Crop Production ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Environment Friendly ◽  
Plant Growth Promoting ◽  
Growth Promoting

The bioagents like Plant Growth Promoting Rhizobacteria (PGPR) and Biocontrol Agents (BCAs) play a crucial role in plant growth promotion, nutrient uptake and suppression of biotic and abiotic stresses. Different researchers have applied these bioagents by various means either through seed treatment or through soil application to prevent various plant diseases. Thus, these non-chemical environment friendly tools can be exploited to enhance crop production.

scholarly journals Comparative and Functional Analyses of Two Sequenced Paenibacillus polymyxa Genomes Provides Insights Into Their Potential Genes Related to Plant Growth-Promoting Features and Biocontrol Mechanisms

2020 ◽  
Vol 11 ◽  
Author(s):  
Jin-Yi Li ◽  
Tan-Tan Gao ◽  
Qi Wang
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Paenibacillus Polymyxa ◽  
Plant Growth Promoting Rhizobacteria ◽  
Gene Clusters ◽  
Systemic Resistance ◽  
Circular Chromosome ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Functional Analyses

Many bacteria belonging to Paenibacillus polymyxa are plant growth-promoting rhizobacteria (PGPR) with the potential to promote plant growth and suppress phytopathogens and have been used as biological control agents (BCAs). However, the growth promotion and biocontrol mechanisms of P. polymyxa have not been thoroughly elucidated thus far. In this investigation, the genome sequences of two P. polymyxa strains, ZF129 and ZF197, with broad anti-pathogen activities and potential for growth promotion were comparatively studied. Comparative and functional analyses of the two sequenced P. polymyxa genomes showed that the ZF129 genome consists of one 5,703,931 bp circular chromosome and two 79,020 bp and 37,602 bp plasmids, designated pAP1 and pAP2, respectively. The complete genome sequence of ZF197 consists of one 5,507,169 bp circular chromosome and one 32,065 bp plasmid, designated pAP197. Phylogenetic analysis revealed that ZF129 is highly similar to two P. polymyxa strains, HY96-2 and SQR-21, while ZF197 is highly similar to P. polymyxa strain J. The genes responsible for secondary metabolite synthesis, plant growth-promoting traits, and systemic resistance inducer production were compared between strains ZF129 and ZF197 as well as other P. polymyxa strains. The results indicated that the variation of the corresponding genes or gene clusters between strains ZF129 and ZF197 may lead to different antagonistic activities of their volatiles or cell-free supernatants against Fusarium oxysporum. This work indicates that plant growth promotion by P. polymyxa is largely mediated by phytohormone production, increased nutrient availability and biocontrol mechanisms. This study provides an in-depth understanding of the genome architecture of P. polymyxa, revealing great potential for the application of this bacterium in the fields of agriculture and horticulture as a PGPR.

scholarly journals Plant-Growth-Promoting Rhizobacteria Emerging as an Effective Bioinoculant to Improve the Growth, Production and Stress Tolerance of Vegetable Crops

2021 ◽  
Vol 22 (22) ◽  
pp. 12245
Author(s):  
Manoj Kumar ◽  
Ved Prakash Giri ◽  
Shipra Pandey ◽  
Anmol Gupta ◽  
Manish Kumar Patel ◽  
...  
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Insect Pests ◽  
Vegetable Consumption ◽  
Plant Growth Promoting Rhizobacteria ◽  
Vegetable Crops ◽  
Vegetable Crop ◽  
Plant Growth Promoting ◽  
Growth Promoting

Vegetable cultivation is a promising economic activity, and vegetable consumption is important for human health due to the high nutritional content of vegetables. Vegetables are rich in vitamins, minerals, dietary fiber, and several phytochemical compounds. However, the production of vegetables is insufficient to meet the demand of the ever-increasing population. Plant-growth-promoting rhizobacteria (PGPR) facilitate the growth and production of vegetable crops by acquiring nutrients, producing phytohormones, and protecting them from various detrimental effects. In this review, we highlight well-developed and cutting-edge findings focusing on the role of a PGPR-based bioinoculant formulation in enhancing vegetable crop production. We also discuss the role of PGPR in promoting vegetable crop growth and resisting the adverse effects arising from various abiotic (drought, salinity, heat, heavy metals) and biotic (fungi, bacteria, nematodes, and insect pests) stresses.

scholarly journals Efficacy of Plant Growth-Promoting Bacteria Bacillus cereus YN917 for Biocontrol of Rice Blast

2021 ◽  
Vol 12 ◽  
Author(s):  
Hu Zhou ◽  
Zuo-hua Ren ◽  
Xue Zu ◽  
Xi-yue Yu ◽  
Hua-jun Zhu ◽  
...  
Keyword(s):  
Disease Prevention ◽  
Plant Growth ◽  
Bacillus Cereus ◽  
Rice Blast ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Plant Growth Promoting Bacteria ◽  
Detached Leaf ◽  
Plant Growth Promoting ◽  
Growth Promoting

Bacillus cereus YN917, obtained from a rice leaf with remarkable antifungal activity against Magnaporthe oryzae, was reported in our previous study. The present study deciphered the possible biocontrol properties. YN917 strain exhibits multiple plant growth-promoting and disease prevention traits, including production of indole-3-acetic acid (IAA), ACC deaminase, siderophores, protease, amylase, cellulase, and β-1,3-glucanase, and harboring mineral phosphate decomposition activity. The effects of the strain YN917 on growth promotion and disease prevention were further evaluated under detached leaf and greenhouse conditions. The results revealed that B. cereus YN917 can promote seed germination and seedling plant growth. The growth status of rice plants was measured from the aspects of rice plumule, radicle lengths, plant height, stem width, root lengths, fresh weights, dry weights, and root activity when YN917 was used as inoculants. YN917 significantly reduced rice blast severity under detached leaf and greenhouse conditions. Genome analysis revealed the presence of gene clusters for biosynthesis of plant promotion and antifungal compounds, such as IAA, tryptophan, siderophores, and phenazine. In summary, YN917 can not only be used as biocontrol agents to minimize the use of chemical substances in rice blast control, but also can be developed as bio-fertilizers to promote the rice plant growth.

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