NMR resonance assignment and dynamics of profilin from Heimdallarchaeota

Abstract The origin of the eukaryotic cell is an unsettled scientific question. The Asgard superphylum has emerged as a compelling target for studying eukaryogenesis due to the previously unseen diversity of eukaryotic signature proteins. However, our knowledge about these proteins is still relegated to metagenomic data and very little is known about their structural properties. Additionally, it is still unclear if these proteins are functionally homologous to their eukaryotic counterparts. Here, we expressed, purified and structurally characterized profilin from Heimdallarchaeota in the Asgard superphylum. The structural analysis shows that while this profilin possesses similar secondary structural elements as eukaryotic profilin, it contains additional secondary structural elements that could be critical for its function and an indication of divergent evolution.

Download Full-text

NMR backbone assignment and dynamics of Profilin from Heimdallarchaeota

10.1101/2020.04.20.050468 ◽

2020 ◽

Author(s):

Syed Razaul Haq ◽

Sabeen Survery ◽

Fredrik Hurtig ◽

Ann-Christin Lindås ◽

Celestine N. Chi

Keyword(s):

Structural Analysis ◽

Structural Properties ◽

Eukaryotic Cell ◽

Backbone Assignment ◽

Metagenomic Data ◽

Divergent Evolution ◽

Structural Elements ◽

Scientific Question ◽

Secondary Structural Elements

AbstractThe origin of the eukaryotic cell is an unsettled scientific question. The Asgard superphylum has emerged as a compelling target for studying eukaryogenesis due to the previously unseen diversity of eukaryotic signature proteins. However, our knowledge about these proteins is still relegated to metagenomic data and very little is known about their structural properties. Additionally, it is still unclear if these proteins are functionally homologous to their eukaryotic counterparts. Here, we expressed, purified and structurally characterized profilin from Heimdallarchaeota in the Asgard superphylum. The structural analysis shows that while this profilin possess similar secondary structural elements as eukaryotic profilin, it contains additional secondary structural elements that could be critical for its function and an indication of divergent evolution.

Download Full-text

Influence of the distribution of the shear walls on the seismic response of the buildings

Pollack Periodica ◽

10.1556/606.2020.15.1.4 ◽

2020 ◽

Vol 15 (1) ◽

pp. 37-44

Author(s):

El Mehdi Echebba ◽

Hasnae Boubel ◽

Oumnia Elmrabet ◽

Mohamed Rougui

Keyword(s):

Structural Analysis ◽

Seismic Response ◽

Natural Frequency ◽

Structural Design ◽

Structural Response ◽

Shear Walls ◽

Structural Elements ◽

Analysis Software ◽

Ansys Apdl ◽

The Impact

Abstract In this paper, an evaluation was tried for the impact of structural design on structural response. Several situations are foreseen as the possibilities of changing the distribution of the structural elements (sails, columns, etc.), the width of the structure and the number of floors indicates the adapted type of bracing for a given structure by referring only to its Geometric dimensions. This was done by studying the effect of the technical design of the building on the natural frequency of the structure with the study of the influence of the distribution of the structural elements on the seismic response of the building, taking into account of the requirements of the Moroccan earthquake regulations 2000/2011 and using the ANSYS APDL and Robot Structural Analysis software.

Download Full-text

Semantic And Structural Properties Of The Banking And Financial Terminology Of The Uzbek Language

The American Journal of Social Science and Education Innovations ◽

10.37547/tajssei/volume02issue08-99 ◽

2020 ◽

Vol 2 (08) ◽

pp. 610-617

Author(s):

Ekaterina Turakulovna Shirinova

Keyword(s):

Structural Analysis ◽

Structural Properties ◽

Human Factor

This article discusses information on the study of terminology in Uzbek and world linguistics. Thematic grouping of banking and financial terms, which play an important role in Uzbek language vocabulary, is considered. The author gives the criteria for the distribution of terms into thematic groups, their peculiar properties examples to substantiate the hypothesis. The paradigmatic relations between the terms of this sphere are indicated. A structural analysis of the banking and financial terms of the Uzbek language is carried out. On the basis of the anthropocentric approach, the role of the human factor in the banking and financial terminology of the Uzbek language is studied. Cognitive metaphors that exist in the terminology are considered.

Download Full-text

Parametric Analysis of the Shear Lag Effect in Tube Structural Systems of Tall Buildings

Applied Sciences ◽

10.3390/app11010278 ◽

2020 ◽

Vol 11 (1) ◽

pp. 278

Author(s):

Ivan Hafner ◽

Anđelko Vlašić ◽

Tomislav Kišiček ◽

Tvrtko Renić

Keyword(s):

Parametric Analysis ◽

Numerical Models ◽

Tall Buildings ◽

Structural Systems ◽

Structural Elements ◽

Structural System ◽

Shear Lag ◽

Shear Lag Effect ◽

Lag Effect ◽

Secondary Structural Elements

Horizontal loads such as earthquake and wind are considered dominant loads for the design of tall buildings. One of the most efficient structural systems in this regard is the tube structural system. Even though such systems have a high resistance when it comes to horizontal loads, the shear lag effect that is characterized by an incomplete and uneven activation of vertical elements may cause a series of problems such as the deformation of internal panels and secondary structural elements, which cumulatively grow with the height of the building. In this paper, the shear lag effect in a typical tube structure will be observed and analyzed on a series of different numerical models. A parametric analysis will be conducted with a great number of variations in the structural elements and building layout, for the purpose of giving recommendations for an optimal design of a tube structural system.

Download Full-text

An automated framework for NMR resonance assignment through simultaneous slice picking and spin system forming

Journal of Biomolecular NMR ◽

10.1007/s10858-014-9828-0 ◽

2014 ◽

Vol 59 (2) ◽

pp. 75-86 ◽

Cited By ~ 4

Author(s):

Ahmed Abbas ◽

Xianrong Guo ◽

Bing-Yi Jing ◽

Xin Gao

Keyword(s):

Resonance Assignment ◽

Spin System ◽

Nmr Resonance Assignment

Download Full-text

In Silico Genetic Robustness Analysis of Secondary Structural Elements in the miRNA Gene

Journal of Molecular Evolution ◽

10.1007/s00239-008-9174-5 ◽

2008 ◽

Vol 67 (5) ◽

pp. 560-569 ◽

Cited By ~ 5

Author(s):

Wenjie Shu ◽

Ming Ni ◽

Xiaochen Bo ◽

Zhiqiang Zheng ◽

Shengqi Wang

Keyword(s):

In Silico ◽

Mirna Gene ◽

Robustness Analysis ◽

Structural Elements ◽

Genetic Robustness ◽

Secondary Structural Elements

Download Full-text

Solid-state MAS NMR resonance assignment methods for proteins

Progress in Nuclear Magnetic Resonance Spectroscopy ◽

10.1016/j.pnmrs.2018.04.002 ◽

2018 ◽

Vol 106-107 ◽

pp. 37-65 ◽

Cited By ~ 12

Author(s):

Victoria A. Higman

Keyword(s):

Solid State ◽

Resonance Assignment ◽

Nmr Resonance Assignment ◽

Mas Nmr

Download Full-text

In silico identification and characterization of sensory motifs in the transcriptional regulators of the ArsR-SmtB family

Metallomics ◽

10.1039/c8mt00082d ◽

2018 ◽

Vol 10 (10) ◽

pp. 1476-1500 ◽

Cited By ~ 4

Author(s):

Rima Roy ◽

Saikat Samanta ◽

Surajit Patra ◽

Nav Kumar Mahato ◽

Rudra P. Saha

Keyword(s):

In Silico ◽

Transcriptional Regulators ◽

Transcriptional Repressors ◽

Structural Elements ◽

In Silico Identification ◽

Metal Efflux ◽

Identification And Characterization ◽

Secondary Structural Elements ◽

Efflux Proteins

The ArsR-SmtB family of transcriptional repressors regulates the transcription of metal-efflux proteins by binding specific metals at a variety of secondary structural elements, called motifs, on the surface of the proteins.

Download Full-text