scholarly journals The architecture of corneal stromal striae on optical coherence tomography and histology in an animal model and in humans

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Pietro Emanuele Napoli ◽  
Matteo Nioi ◽  
Ernesto d’Aloja ◽  
Francesco Loy ◽  
Maurizio Fossarello
Keyword(s):  
Animal Model ◽  
Optical Coherence Tomography ◽  
Image Analysis ◽  
Corneal Stroma ◽  
Optical Coherence ◽  
Female Adult ◽  
Truss Bridge ◽  
Frequent Observation

AbstractThe purpose of this study was to use a portable optical coherence tomography (OCT) for characterization of corneal stromal striae (CSS) in an ovine animal model and human corneas with histological correlation, in order to evaluate their architectural pattern by image analysis. Forty-six eyes from female adult sheep (older than 2 years), and 12 human corneas, were included in our study. The eyes were examined in situ by a portable OCT, without enucleation. All OCT scans were performed immediately after death, and then the eyes were delivered to a qualified histology laboratory. In the ovine animal model, CSS were detected with OCT in 89.1% (41/46) of individual scans and in 93.4% (43/46) of histological slices. In human corneas, CSS were found in 58.3% (7/12) of cases. In both corneal types, CSS appeared as “V”- or “X”-shaped structures, with very similar angle values of 70.8° ± 4° on OCT images and 71° ± 4° on histological slices (p ≤ 0.01). Data analysis demonstrated an excellent degree of reproducibility and inter-rater reliability of measurements (p < 0.001). The present study demonstrated that by using a portable OCT device, CSS can be visualized in ovine and human corneas. This finding suggests their generalized presence in various mammals. The frequent observation, close to 60%, of such collagen texture in the corneal stroma, similar to a ‘truss bridge’ design, permits to presume that it plays an important structural role, aimed to distribute tensile and compressive forces in various directions, conferring resilience properties to the cornea.

In Situ Ultrahigh-resolution Optical Coherence Tomography Characterization of Eye Bank Corneal Tissue Processed for Lamellar Keratoplasty

Cornea ◽  
2008 ◽  
Vol 27 (7) ◽  
pp. 802-810 ◽  
Author(s):  
Jamin S Brown ◽  
Danling Wang ◽  
Xiaoli Li ◽  
Florence Baluyot ◽  
Bernie Iliakis ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Lamellar Keratoplasty ◽  
Corneal Tissue ◽  
Optical Coherence ◽  
Eye Bank ◽  
Ultrahigh Resolution

scholarly journals Spatially-resolved in-situ quantification of biofouling using optical coherence tomography (OCT) and 3D image analysis in a spacer filled channel

2017 ◽  
Vol 524 ◽  
pp. 673-681 ◽  
Author(s):  
Luca Fortunato ◽  
Szilárd Bucs ◽  
Rodrigo Valladares Linares ◽  
Corrado Cali ◽  
Johannes S. Vrouwenvelder ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Image Analysis ◽  
Optical Coherence ◽  
3D Image ◽  
3D Image Analysis ◽  
Spatially Resolved

scholarly journals Morpho-molecular ex vivo detection and grading of non-muscle-invasive bladder cancer using forward imaging probe based multimodal optical coherence tomography and Raman spectroscopy

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1445-1456 ◽  
Author(s):  
Fabian Placzek ◽  
Eliana Cordero Bautista ◽  
Simon Kretschmer ◽  
Lara M. Wurster ◽  
Florian Knorr ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Coherence Tomography ◽  
Imaging System ◽  
Ex Vivo ◽  
Large Field ◽  
Optical Coherence ◽  
Fiber Optic Probe ◽  
Optic Probe ◽  
Muscle Invasive

Characterization of bladder biopsies, using a combined fiber optic probe-based optical coherence tomography and Raman spectroscopy imaging system that allows a large field-of-view imaging and detection and grading of cancerous bladder lesions.

scholarly journals Characterization of Optical Coherence Tomography Images for Colon Lesion Differentiation under Deep Learning

2021 ◽  
Vol 11 (7) ◽  
pp. 3119
Author(s):  
Cristina L. Saratxaga ◽  
Jorge Bote ◽  
Juan F. Ortega-Morán ◽  
Artzai Picón ◽  
Elena Terradillos ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Deep Learning ◽  
Data Augmentation ◽  
Optical Biopsy ◽  
Colon Polyps ◽  
Optical Coherence ◽  
Colon Lesion ◽  
Patient Prognosis ◽  
B Scan Images

(1) Background: Clinicians demand new tools for early diagnosis and improved detection of colon lesions that are vital for patient prognosis. Optical coherence tomography (OCT) allows microscopical inspection of tissue and might serve as an optical biopsy method that could lead to in-situ diagnosis and treatment decisions; (2) Methods: A database of murine (rat) healthy, hyperplastic and neoplastic colonic samples with more than 94,000 images was acquired. A methodology that includes a data augmentation processing strategy and a deep learning model for automatic classification (benign vs. malignant) of OCT images is presented and validated over this dataset. Comparative evaluation is performed both over individual B-scan images and C-scan volumes; (3) Results: A model was trained and evaluated with the proposed methodology using six different data splits to present statistically significant results. Considering this, 0.9695 (±0.0141) sensitivity and 0.8094 (±0.1524) specificity were obtained when diagnosis was performed over B-scan images. On the other hand, 0.9821 (±0.0197) sensitivity and 0.7865 (±0.205) specificity were achieved when diagnosis was made considering all the images in the whole C-scan volume; (4) Conclusions: The proposed methodology based on deep learning showed great potential for the automatic characterization of colon polyps and future development of the optical biopsy paradigm.

scholarly journals Accidental Corneal Intrastromal Intraocular Lens Implantation with the Wound-Assisted Technique and Clinical Course with Anterior Segment Optical Coherence Tomography

2021 ◽  
pp. 283-287
Author(s):  
Jo Moriya ◽  
Shinichi Sakamoto ◽  
Satoru Inoda ◽  
Hidenori Takahashi ◽  
Hidetoshi Kawashima
Keyword(s):  
Optical Coherence Tomography ◽  
Intraocular Lens ◽  
Anterior Segment ◽  
Corneal Stroma ◽  
Age Related Macular Degeneration ◽  
Optical Coherence ◽  
Corrected Visual Acuity ◽  
Age Related ◽  
Intracameral Injection ◽  
Air Tamponade

Accidental intraocular lens (IOL) implantation into the corneal stroma is a rare clinical entity that can occur during the wound-assisted technique. In this report, we describe a case of an 81-year-old man who underwent cataract surgery in which the IOL was implanted into the corneal stroma with the wound-assisted technique, and we present changes in anterior segment optical coherence tomography. The IOL was removed and reinserted after widening the incision. Air tamponade was created by intracameral injection. An anterior chamber tap was performed 10 h later to reduce increased intraocular pressure. Interlayer separation of the corneal stroma was confirmed 30 min postoperatively but was corrected 4 days later. The patient’s best-corrected visual acuity (BCVA) was logMAR 0.30, and he had been diagnosed with age-related macular degeneration before surgery. Although the opacity of the corneal stroma persisted, BCVA improved to logMAR 0. When using the wound-assisted technique for IOL insertion, surgeons should take care not to implant the IOL into the corneal stroma.

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