scholarly journals Thermal and non-thermal effects of capacitive–resistive electric transfer application on different structures of the knee: a cadaveric study

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jacobo Rodríguez-Sanz ◽  
Albert Pérez-Bellmunt ◽  
Carlos López-de-Celis ◽  
Orosia María Lucha-López ◽  
Vanessa González-Rueda ◽  
...  
Keyword(s):  
Low Power ◽  
Electric Current ◽  
Thermal Effects ◽  
Sports Medicine ◽  
Current Flow ◽  
In Vitro Study ◽  
Science Data ◽  
Fresh Frozen ◽  
Inflammatory Processes

AbstractCapacitive–resistive electric transfer therapy is used in physical rehabilitation and sports medicine to treat muscle, bone, ligament and tendon injuries. The purpose is to analyze the temperature change and transmission of electric current in superficial and deep knee tissues when applying different protocols of capacitive–resistive electric transfer therapy. Five fresh frozen cadavers (10 legs) were included in this study. Four interventions (high/low power) were performed for 5 min by a physiotherapist with experience. Dynamic movements were performed to the posterior region of the knee. Capsular, intra-articular and superficial temperature were recorded at 1-min intervals and 5 min after the treatment, using thermocouples placed with ultrasound guidance. The low-power protocols had only slight capsular and intra-capsular thermal effects, but electric current flow was observed. The high-power protocols achieved a greater increase in capsular and intra-articular temperature and a greater current flow than the low-power protocols. The information obtained in this in vitro study could serve as basic science data to hypothesize capsular and intra-articular knee recovery in living subjects. The current flow without increasing the temperature in inflammatory processes and increasing the temperature of the tissues in chronic processes with capacitive–resistive electric transfer therapy could be useful for real patients.

scholarly journals Redefining Knee Balance in a Medially Stabilized Prosthesis: An In-Vitro Study

2017 ◽  
Vol 11 (1) ◽  
pp. 1165-1172
Author(s):  
Philippe Van Overschelde ◽  
Vera Pinskerova ◽  
Peter P. Koch ◽  
Christophe Fornasieri ◽  
Sandro Fucentese
Keyword(s):  
Soft Tissue ◽  
Soft Tissues ◽  
In Vitro Study ◽  
Lateral Release ◽  
Soft Tissue Release ◽  
Iliotibial Band ◽  
Soft Tissue Envelope ◽  
Medial Side ◽  
Fresh Frozen

Background: To date, there is still no consensus on what soft tissues must be preserved and what structures can be safely released during total knee arthroplasty (TKA) with a medially stabilized implant. Objective: The aim of this study was to analyze the effect of a progressive selective release of the medial and lateral soft tissues in a knee implanted with a medially stabilized prosthesis. Method: Six cadaveric fresh-frozen full leg specimens were tested. In each case, kinematic pattern and mediolateral laxity were measured in three stages: firstly, prior to implantation; secondly, after the implantation of the trial components, but before any soft tissue release; and thirdly, progressively as soft tissue was released with the trial implant in place. The incremental impact of each selective release on knee balance was then analyzed. Results: In all cases sagittal stability was not affected by the progressive release of the lateral soft tissue envelope. It was possible to perform progressive lateral release provided the anterior one-third of the iliotibial band (ITB) remained intact. Progressive medial release could be performed on the medial side provided the anterior fibers of the superficial medial collateral ligament (sMCL) remained intact. Conclusion: The medially conforming implant remains stable provided the anterior fibers of sMCL and the anterior fibers of the ITB remain intact. The implant’s sagittal stability is mainly dependent on its medial ball-in-socket design.

Effect of Donation Time on Platelet Concentrates and Fresh-Frozen Plasma. An in vitro Study

Vox Sanguinis ◽  
1989 ◽  
Vol 56 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Yang O. Huh ◽  
Benjamin Lichtiger ◽  
Geoffrey G. Giacco ◽  
Vincent F. Guinee ◽  
Benjamin Drewinko
Keyword(s):  
In Vitro Study ◽  
Fresh Frozen Plasma ◽  
Vitro Study ◽  
Platelet Concentrates ◽  
Fresh Frozen ◽  
Frozen Plasma

scholarly journals The effects of disodium pamidronate on human polymorphonuclear leukocytes and platelets: An in vitro study

2009 ◽  
Vol 14 (3) ◽  
Author(s):  
Eleonora Salvolini ◽  
Monia Orciani ◽  
Arianna Vignini ◽  
Roberto Primio ◽  
Laura Mazzanti
Keyword(s):  
Nitric Oxide ◽  
In Vitro Study ◽  
Protective Mechanism ◽  
No Production ◽  
Myeloperoxidase Activity ◽  
Inflammatory Processes ◽  
Anti Inflammatory ◽  
Constitutive Nitric Oxide Synthase

AbstractRecent reports have indicated that, as well as having antiresorptive effects, bisphosphonates could have an application as anti-inflammatory drugs. Our aim was to investigate whether this anti-inflammatory action could be mediated by the nitric oxide (NO) released by the leukocytes migrating to the site of inflammation. In particular, we investigated in vitro the intracellular calcium concentration ([Ca2+]i), the level of NO released by PMN and platelets, and the PMN myeloperoxidase activity after incubation with disodium pamidronate, since there was a postulated modulatory effect of this aminosubstituted bisphosphonate on leukocytes both in vitro and in vivo. Our data shows that the pamidronate treatment provoked a significant increase in the [Ca2+]i parallel to the enhancement in NO release, suggesting a possible activation of constitutive nitric oxide synthase, while the myeloperoxidase activity was significantly reduced. In conclusion, we hypothesized that treatment with pamidronate could stimulate NO-production by cells present near the bone compartment, thus constituting a protective mechanism against bone resorption occurring during inflammation. In addition, PMN- and platelet-derived NO could act as a negative feed-back signal to restrict the inflammatory processes.

scholarly journals Antioxidant activity of various plant sprouts extracts depending on the processing method of plant material – an in vitro study (in press)

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Anna Muzykiewicz-Szymańska ◽  
Joanna Zielonka-Brzezicka ◽  
Joanna Siemak ◽  
Adam Klimowicz
Keyword(s):  
Antioxidant Activity ◽  
Mung Bean ◽  
Plant Material ◽  
In Vitro Study ◽  
Natural Antioxidants ◽  
Processing Method ◽  
Antioxidant Potential ◽  
Ultrasound Assisted Extraction ◽  
Fresh Frozen

SummaryIntroduction: Due to the content of nutrients and taste attributes, the plant sprouts could become a valuable component of a healthy diet.Objective: The aim of the study was to evaluate the antioxidant potential of various sprouts extracts depending on the processing method of the plant material.Methods: The extracts in 96%(v/v) ethanol were prepared from fresh, frozen, dry and homogenized sprouts of alfalfa, broccoli, radish, lentil, mung bean, beetroot, sunflower, and kale, using ultrasound-assisted extraction. The antioxidant potential and total polyphenols content were evaluated by the in vitro methods.Results: The highest antioxidant activity was observed for beetroot and kale as well as broccoli sprouts extracts. The lower potential, in general, was observed in the case of alfalfa, lentil, and mung bean sprouts samples. The lowest activity was observed most frequently for the extracts from dry material, whereas the highest for homogenized as well as frozen and fresh.Conclusion: The plant sprouts, particularly beetroot, kale, and broccoli, could be a valuable source of natural antioxidants.

Thermal effects and morphological aspects of varying Er:YAG laser energy on demineralized dentin removal: an in vitro study

2014 ◽  
Vol 30 (4) ◽  
pp. 1231-1236 ◽  
Author(s):  
Walter Raucci-Neto ◽  
Carla Raquel dos Santos ◽  
Fabrício Augusto de Lima ◽  
Jesus Djalma Pécora ◽  
Luciano Bachmann ◽  
...  
Keyword(s):  
Thermal Effects ◽  
Er:Yag Laser ◽  
Laser Energy ◽  
In Vitro Study ◽  
Vitro Study ◽  
Morphological Aspects ◽  
Demineralized Dentin

scholarly journals Are the Effects in Temperature and Current Flow Dependent on the Areas of Electrode Application of Capacitive-resistive Electric Transfer on the Shoulder? A Cadaveric Study

2020 ◽  
Author(s):  
Jacobo Rodríguez-Sanz ◽  
Carlos López-de-Celis ◽  
César Hidalgo-García ◽  
Max Canet-Vintró ◽  
Pablo Fanlo-Mazas ◽  
...  
Keyword(s):  
High Power ◽  
Temperature Increase ◽  
Current Flow ◽  
In Vitro Study ◽  
Supraspinatus Tendon ◽  
Lateral Position ◽  
Cross Sectional ◽  
Significant Difference ◽  
Glenohumeral Capsule

Abstract Background Impingement syndrome is currently estimated to represent 60% of all shoulder pain disorders. Capacitive-Resistive electric transfer therapy is aimed to provoke temperature and current flow changes in superficial and deep tissues. This in vitro study has evaluated the variation of temperature and current flow in the shoulder tissues during two different areas of application of the movable capacitive-resistive electric transfer electrode. Methods A cross-sectional study designed, five fresh cryopreserved cadavers (10 shoulders) were included in this study. Four interventions (capacitive and resistive modes; low- and high-power) were performed for 5 minutes each by a diathermy “T-Plus” device in two shoulder regions: postero-superior and antero-lateral. Supraspinatus tendon, glenohumeral capsule and superficial temperatures were recorded at 1-min intervals and 5 min after treatment. Results A statistically significant difference was found only for the superficial area and time interaction, with high power-resistive application at the postero-superior shoulder area (P < 0.035). All the applications showed a final temperature increase compared with the basal data, in all the application points. Superficial temperature in the high power-resistive application showed the greatest percent increase (42.93% ± 22.58), followed by the temperature in the tendon area with the same high power-resistive application (22.97% ± 14.70). The high power-resistive application showed the greatest percent of temperature increase in the applications, reaching 65.9% ± 22.96 at 5-min at the superficial level, and 32% ± 24.25 at 4-min at the level of the supraspinatus tendon. At the capsule level, high power-resistive was also the application that showed the greatest percent of increase, with 21.52% ± 16.16. The application with the lowest percent of temperature increase was the low power-capacitive, with a mean value of 4.86% at supraspinatus tendon level and 7.47% at capsular level. Conclusion The shoulder postero-superior or antero-lateral areas of application of capacitive-resistive electric transfer did not cause statistically significant differences in the temperature changes in either supraspinatus tendon or glenohumeral capsule tissues in cadaveric samples. The high power-resistive application in the postero-superior area significantly increased superficial temperature compared with the same application in the antero-lateral position area.

scholarly journals Low-power laser irradiation inhibits arecoline-induced fibrosis: an in vitro study

2017 ◽  
Vol 9 (1) ◽  
pp. 38-42 ◽  
Author(s):  
Mei-Chun Yeh ◽  
Ker-Kong Chen ◽  
Min-Hsuan Chiang ◽  
Chia-Hsin Chen ◽  
Ping-Ho Chen ◽  
...  
Keyword(s):  
Low Power ◽  
Laser Irradiation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Power Laser

The Strength of Achilles Tendon Repair: An In Vitro Study of the Biomechanical Behavior in Human Cadaver Tendons

1995 ◽  
Vol 16 (4) ◽  
pp. 191-195 ◽  
Author(s):  
Thomas W. Watson ◽  
Kenneth A. Jurist ◽  
King H. Yang ◽  
Kun-Ling Shen
Keyword(s):  
Achilles Tendon ◽  
Tendon Repair ◽  
In Vitro Study ◽  
Current Standard ◽  
Initial Strength ◽  
Biomechanical Behavior ◽  
Achilles Tendon Repair ◽  
Fresh Frozen ◽  
Tendon Ruptures

Eighteen fresh frozen human Achilles tendons were used to test the ultimate strength of repaired tendon “ruptures.” Three methods, the Kessler, the Bunnell, and the locking loop, were used to test the initial strength of Achilles tendon repair. The Kessler and Bunnell methods are current standard clinical configurations described for Achilles tendon repair. Under uniform and standardized laboratory conditions, the specimens were loaded to failure. The locking loop suture method was substantially stronger than either of the other two standard configurations. The latter two did not differ significantly from each other. The results of this study may be clinically relevant in terms of the choice of the repair method for surgically treated Achilles tendon ruptures.

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