scholarly journals Clemastine improves electrophysiologic and histomorphometric changes through promoting myelin repair in a murine model of compression neuropathy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jung Il Lee ◽  
Jong Woong Park ◽  
Kyung Jun Lee ◽  
Duk Hee Lee
Keyword(s):  
Sciatic Nerve ◽  
Functional Recovery ◽  
Murine Model ◽  
Demyelinating Disease ◽  
Surgical Decompression ◽  
Myelin Proteins ◽  
Compression Phase ◽  
Myelin Repair ◽  
Compression Neuropathy ◽  
G Ratio

AbstractCompression neuropathies are common and debilitating conditions that result in variable functional recovery after surgical decompression. Recent drug repurposing studies have verified that clemastine promotes functional recovery through enhancement of myelin repair in demyelinating disease. We investigated the utility of clemastine as a treatment for compression neuropathy using a validated murine model of compression neuropathy encircling the compression tube around the sciatic nerve. Mice received PBS or clemastine solution for 6 weeks of compression phase. Mice taken surgical decompression received PBS or clemastine solution for 2 weeks of decompression phase. Electrodiagnostic, histomorphometric, and Western immunoblotting analyses were performed to verify the effects of clemastine. During the compression phase, mice treated with clemastine had significantly decreased latency and increased amplitude compared to untreated mice that received PBS. Histomorphometric analyses revealed that mice treated with clemastine had significantly higher proportions of myelinated axons, thicker myelin, and a lower G-ratio. The expression levels of myelin proteins, including myelin protein zero and myelin associated glycoprotein, were higher in mice treated with clemastine. However, the electrophysiologic and histomorphometric improvements were observed regardless of clemastine treatment in mice taken surgical decompression. Mice treated with clemastine during compression of the sciatic nerve demonstrated that clemastine treatment attenuated electrophysiologic and histomorphometric changes caused by compression through promoting myelin repair.

Triptolide improves nerve regeneration and functional recovery following crush injury to rat sciatic nerve

2014 ◽  
Vol 561 ◽  
pp. 198-202 ◽  
Author(s):  
Yong-Guang Zhang ◽  
Qing-Song Sheng ◽  
Hong-Kun Wang ◽  
Li Lv ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Functional Recovery ◽  
Crush Injury ◽  
Rat Sciatic Nerve

Functional Recovery and Vitamin E Level Following Sciatic Nerve Crush Injury in Normal and Diabetic Rats

1998 ◽  
Vol 96 (3-4) ◽  
pp. 245-254 ◽  
Author(s):  
Khalaf Al Moutaery ◽  
Mohammed Arshaduddin ◽  
Mohammad Tariq ◽  
Saleh Al Deeb
Keyword(s):  
Vitamin E ◽  
Sciatic Nerve ◽  
Functional Recovery ◽  
Diabetic Rats ◽  
Crush Injury ◽  
Sciatic Nerve Crush ◽  
Nerve Crush ◽  
Nerve Crush Injury

scholarly journals PC198. Arterial Calcification Decreases Functional Recovery in a Murine Model of Hind Limb Ischemia

2015 ◽  
Vol 61 (6) ◽  
pp. 171S
Author(s):  
Sara L. Zettervall ◽  
Stephanie D. Monk ◽  
Xue-Lin Wang ◽  
Tonghui Lin ◽  
Raul J. Guzman
Keyword(s):  
Functional Recovery ◽  
Murine Model ◽  
Hind Limb ◽  
Limb Ischemia ◽  
Arterial Calcification ◽  
Hind Limb Ischemia

scholarly journals Salvia officinalis, Rosmarinic and Caffeic Acids Attenuate Neuropathic Pain and Improve Function Recovery after Sciatic Nerve Chronic Constriction in Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Zineb El Gabbas ◽  
Kenza Bezza ◽  
Jawad Laadraoui ◽  
Mehdi Ait Laaradia ◽  
Aaziz Kebbou ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Functional Recovery ◽  
Thermal Sensation ◽  
Biological Activities ◽  
Hematological Parameters ◽  
Thermal Hyperalgesia ◽  
Salvia Officinalis ◽  
Histopathological Analysis ◽  
Phenolic Constituents

The leaves of Salvia officinalis L. have a traditional reputation for the management of pain in Morocco. This study was conducted to investigate the curative effects of Salvia officinalis (SO) and its major constituents Rosmarinic (ROS) and Caffeic acids (CAF) on peripheral neuropathic pain in mice. Chronic constriction injury (CCI) was induced in mice, and neuropathic pain behaviors tests were evaluated by mechanical, chemical, thermal sensation tests and functional recovery of the sciatic nerve at different time intervals, i.e., (day 0, 1, 7, 14, and 21). Ethanolic extract of SO (100 and 200 mg/kg, p.o.), ROS (10 and 20 mg/kg, i.p.), CAF (30 and 40 mg/kg, i.p.), and CLOM (5 mg/kg, i.p., a positive control) was given for 21 days after surgery. Hematological and biochemical parameters were also measured as well as histopathological analysis. CCI produced significant development in mechanical and thermal hyperalgesia, cold allodynia, and rise in the sciatic functional index in mice. Chronic treatments with SO extract, ROS, CAF, and CLOM for 3 weeks significantly increased mechanical sensibility, cold, and thermal withdrawal latency and enhanced functional recovery of the injured nerve. The same treatments remarkably ameliorated hematological parameters and did not alter biochemical levels. The histopathological findings had revealed the protective effect of SO, ROS, and CAF against the CCI-induced damage. Our data support the use of SO in folk medicine to alleviate pain. Their main phenolic constituents could be promising antineuropathic compounds, which may be attributed to their biological activities including anti-inflammatory, antioxidant, and neuroprotective effects. SO leaves may be a good candidate to treat neuropathic pain.

scholarly journals Functional Recovery via Preregenerated Nerve Graft in Rat Sciatic Nerve Injury Model

Neurosurgery ◽  
2019 ◽  
Vol 66 (Supplement_1) ◽  
Author(s):  
Umang Khandpur ◽  
Ying Yan ◽  
Wilson Zachary Ray ◽  
Matthew R MacEwan
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Functional Recovery ◽  
Peripheral Nerve Injury ◽  
Muscle Force ◽  
Nerve Graft ◽  
Tissue Loss ◽  
Limb Amputation ◽  
Percent Recovery

Abstract INTRODUCTION Patients who have experienced major tissue loss with peripheral nerve injury (eg, limb amputation) may be offered composite tissue allotransplantation (CTA). The return of sensory ability and cosmetic component of CTAs make them an attractive alternative to prosthetic devices. Unfortunately, robust reinnervation especially over great distances remains an issue for hand allotransplants. In this study, we introduce a preregenerated nerve graft to shorten the distance and therefore time to terminal tissue reinnervation, which could improve the utility of CTAs. METHODS A total of 18 rats weighing 250 to 300 gm each were randomized into 1 of 3 groups: baseline, fresh, or preregenerated. The baseline group underwent sham surgery to obtain baseline functional data. The fresh and preregenerated groups both underwent grafting of the sciatic nerve but the preregenerated group utilized 8-wk preregenerated grafts. At postperative week 8 from distal neurorrhaphy, both groups underwent terminal functional testing via EMG and evoked muscle force. RESULTS The preregenerated group had significantly greater mean EMG (P < .05) and maximum tetanic muscle force values (P < .05) than the fresh group. Mean percent recovery in EMG for the fresh group was 21.95% compared with 81.79% in the preregenerated group. Mean percent recovery in muscle force was 9.46% and 33.15%, respectively. CONCLUSION The results of this study provide a novel approach to enhance final functional recovery after peripheral nerve injury. The current practice of constructing a nerve stump may be improved by grafting a nerve segment at the time of injury and allowing it to preregenerate into local musculature so that if a CTA is later performed, an expedited and more robust reinnervation could be accomplished.

Rosuvastatin enhanced functional recovery after sciatic nerve injury in the rat

2020 ◽  
Vol 882 ◽  
pp. 173260 ◽  
Author(s):  
Arash Abdolmaleki ◽  
Saber Zahri ◽  
Abolfazl Bayrami
Keyword(s):  
Sciatic Nerve ◽  
Nerve Injury ◽  
Functional Recovery ◽  
Sciatic Nerve Injury

scholarly journals Imaging the Proangiogenic Effects of Cardiovascular Drugs in a Diabetic Model of Limb Ischemia

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
J. L. Goggi ◽  
A. Haslop ◽  
R. Boominathan ◽  
K. Chan ◽  
V. Soh ◽  
...  
Keyword(s):  
Disease Progression ◽  
Functional Recovery ◽  
Pet Imaging ◽  
Murine Model ◽  
Hind Limb ◽  
Immunohistochemical Analysis ◽  
Limb Ischemia ◽  
Vegf Expression ◽  
Vascular Volume ◽  
Hind Limb Ischemia

Purpose. Peripheral artery disease (PAD) causes narrowing of arteries in the limbs, leading to tissue ischemia, gangrene, and eventually limb amputation. The presence of diabetes greatly exacerbates the course of PAD, accounting for the majority of lower limb amputations. Therapeutic strategies focussing on macrovascular repair are less effective in diabetic patients where smaller vessels are affected, and proangiogenic therapies offer a viable adjunct to improve vascularisation in these at risk individuals. The purpose of the current study was to assess the proangiogenic effects of drugs routinely used to treat cardiovascular disease in a diabetic murine model of hind limb ischemia longitudinally using multimodal imaging. Procedures. Diabetic mice underwent surgical intervention to induce hind limb ischemia and were treated with simvastatin, metformin, or a combination orally for 28 days and compared to diabetic and nondiabetic mice. Neovascularisation was assessed using [18F]FtRGD PET imaging, and macrovascular volume was assessed by quantitative time of flight MRI. At each imaging time point, VEGF expression and capillary vessel density were quantified using immunohistochemical analysis, and functional recovery and disease progression were assessed. Results. Combined use of simvastatin and metformin significantly increased neovascularisation above levels measured with either treatment alone. Early angiogenic events were accurately assessed using PET [18F]FtRGD, showing maximal retention in the ischemic hind limb by day 8, which translated to a sustained increase in vascular volume at later time points. Immunohistochemical analysis shows that combined therapy significantly increased VEGF expression and capillary density (CD31+) in a similar time course and also slowed disease progression while simultaneously improving functional foot use. Conclusions. Combined treatment with simvastatin and metformin led to a significant improvement in limb angiogenesis, vascular volume, and sustained functional recovery in a diabetic murine model of HLI. PET imaging with [18F]FtRGD provides a robust method for early detection of these proangiogenic effects preclinically and may be useful for the assessment of proangiogenic therapies used clinically to treat diabetic PAD patients.

Sign in / Sign up

Export Citation Format

Share Document