scholarly journals Author Correction: Differential microbial responses to antibiotic treatments by insecticide-resistant and susceptible cockroach strains (Blattella germanica L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zachery M. Wolfe ◽  
Michael E. Scharf
Keyword(s):  
Blattella Germanica ◽  
Microbial Responses

scholarly journals Differential microbial responses to antibiotic treatments by insecticide-resistant and susceptible cockroach strains (Blattella germanica L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Zachery M. Wolfe ◽  
Michael E. Scharf
Keyword(s):  
Control Diet ◽  
Blattella Germanica ◽  
German Cockroach ◽  
Past Research ◽  
Gut Bacteria ◽  
Microbial Composition ◽  
Bacterial Dna ◽  
Urban Pest ◽  
Microbial Responses ◽  
Higher Doses

AbstractThe German cockroach (Blattella germanica L.) is a major urban pest worldwide and is known for its ability to resist insecticides. Past research has shown that gut bacteria in other insects can metabolize xenobiotics, allowing the host to develop resistance. The research presented here determined differences in gut microbial composition between insecticide-resistant and susceptible German cockroaches and compared microbiome changes with antibiotic treatment. Cockroaches received either control diet or diet plus kanamycin (KAN) to quantify shifts in microbial composition. Additionally, both resistant and susceptible strains were challenged with diets containing the insecticides abamectin and fipronil in the presence and absence of antibiotic. In both strains, KAN treatment reduced feeding, leading to higher doses of abamectin and fipronil being tolerated. However, LC50 resistance ratios between resistant and susceptible strains decreased by half with KAN treatment, suggesting gut bacteria mediate resistance. Next, whole guts were isolated, bacterial DNA extracted, and 16S MiSeq was performed. Unlike most bacterial taxa, Stenotrophomonas increased in abundance in only the kanamycin-treated resistant strain and was the most indicative genus in classifying between control and kanamycin-treated cockroach guts. These findings provide unique insights into how the gut microbiome responds to stress and disturbance, and important new insights into microbiome-mediated insecticide resistance.

Comparison of the effect of ABC-transporters inhibitor verapamil at different ways of intake to the german сосkroach Blattella germanica (l.) (Blattodea: Ectobiidae)

2019 ◽  
pp. 53-59
Author(s):  
Olga Yurevna Eremina ◽  
◽  
Veronika Valentinovna Olifer ◽  
Yuliya Vladimirovna Lopatina ◽  
◽  
...  
Keyword(s):  
Abc Transporters ◽  
Blattella Germanica

Two putative fatty acid synthetic genes of BgFas3 and BgElo1 are responsible for respiratory waterproofing in Blattella germanica

2021 ◽  
Author(s):  
Xiao‐Jin Pei ◽  
Tian‐Tian Bai ◽  
Zhan‐Feng Zhang ◽  
Nan Chen ◽  
Sheng Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Blattella Germanica ◽  
Synthetic Genes

scholarly journals Soil age and soil organic carbon content shape biochemical responses to multiple freeze–thaw events in soils along a postmining agricultural chronosequence

2021 ◽  
Author(s):  
Christoph Rosinger ◽  
Michael Bonkowski
Keyword(s):  
Microbial Biomass ◽  
Organic Carbon ◽  
Soil Organic Carbon ◽  
Carbon Content ◽  
Organic Carbon Content ◽  
Freeze Thaw ◽  
Biochemical Responses ◽  
Microbial Responses ◽  
Soil Age ◽  
Carbon Losses

AbstractFreeze–thaw (FT) events exert a great physiological stress on the soil microbial community and thus significantly impact soil biogeochemical processes. Studies often show ambiguous and contradicting results, because a multitude of environmental factors affect biogeochemical responses to FT. Thus, a better understanding of the factors driving and regulating microbial responses to FT events is required. Soil chronosequences allow more focused comparisons among soils with initially similar start conditions. We therefore exposed four soils with contrasting organic carbon contents and opposing soil age (i.e., years after restoration) from a postmining agricultural chronosequence to three consecutive FT events and evaluated soil biochgeoemical responses after thawing. The major microbial biomass carbon losses occurred after the first FT event, while microbial biomass N decreased more steadily with subsequent FT cycles. This led to an immediate and lasting decoupling of microbial biomass carbon:nitrogen stoichiometry. After the first FT event, basal respiration and the metabolic quotient (i.e., respiration per microbial biomass unit) were above pre-freezing values and thereafter decreased with subsequent FT cycles, demonstrating initially high dissimilatory carbon losses and less and less microbial metabolic activity with each iterative FT cycle. As a consequence, dissolved organic carbon and total dissolved nitrogen increased in soil solution after the first FT event, while a substantial part of the liberated nitrogen was likely lost through gaseous emissions. Overall, high-carbon soils were more vulnerable to microbial biomass losses than low-carbon soils. Surprisingly, soil age explained more variation in soil chemical and microbial responses than soil organic carbon content. Further studies are needed to dissect the factors associated with soil age and its influence on soil biochemical responses to FT events.

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