scholarly journals Optimization of pineapple juice amount used as a negative oral contrast agent in magnetic resonance cholangiopancreatography

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Matteo Renzulli ◽  
Daniele Caretti ◽  
Irene Pettinari ◽  
Maurizio Biselli ◽  
Stefano Brocchi ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Cholangiopancreatography ◽  
Absorption Spectrometry ◽  
Final Concentration ◽  
Oral Contrast ◽  
Pineapple Juice ◽  
Dose Oral ◽  
Vitro Analysis ◽  
In Vitro Analysis

AbstractTo evaluate the potential variability of Manganese (Mn2+) in commercial pineapple juice (PJ) produced in different years and to identify the optimal Mn2+ concentration in the correct amount of PJ to be administered prior to Magnetic Resonance Cholangiopancreatography (MRCP) in order to suppress the gastroduodenal (GD) liquid signal. The Mn2+ concentration in PJ produced in different years was defined using Atomic Absorption Spectrometry. The optimal Mn2+ concentration and the amount of PJ, were estimated in an in-vitro analysis, and were then prospectively tested in a population of patients who underwent MRCP. The results were compared with those achieved with the previous standard amount of PJ used in a similar population. The concentrations of Mn2+ in commercial PJ produced in different years did not differ. A total amount of 150 ml (one glass) of PJ having a high Mn2+ content (2.37 mg/dl) was sufficient for the suppression of the GD liquid signal, despite the additional dilution caused by GD liquids since it led to a final concentration of Mn2+ of 0.5–1.00 mg/dl. The optimized single-dose oral administration of 150 ml (approximately one glass) of PJ having a high Mn2+ concentration prior to MRCP was adequate to guarantee the correct amount of Mn2+ to suppress the GD signal.

Work in Progress: In Vitro Analysis of Pleural Fluid Analogs by Proton Magnetic Resonance Preliminary Studies at 1.5T

1987 ◽  
Vol 22 (5) ◽  
pp. 382-387 ◽  
Author(s):  
PETER VOCK ◽  
LAURENCE W. HEDLUND ◽  
ROBERT J. HERFKENS ◽  
ERIC L. EFFMANN ◽  
MARK A. BROWN ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Pleural Fluid ◽  
Proton Magnetic Resonance ◽  
Work In Progress ◽  
Vitro Analysis ◽  
In Vitro Analysis

Diagnosis of gallstone composition in magnetic resonance imaging: in vitro analysis

2002 ◽  
Vol 41 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Minoru Ukaji ◽  
Masaaki Ebara ◽  
Yukihiro Tsuchiya ◽  
Hirotoshi Kato ◽  
Hiroyuki Fukuda ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Resonance Imaging ◽  
Vitro Analysis ◽  
Gallstone Composition ◽  
In Vitro Analysis

scholarly journals THE ROLE OF BLACK TEA AND PINEAPPLE JUICE AS NEGATIVE ORAL CONTRASTS ON MAGNETIC RESONANCE CHOLANGIOPANCREATOGRAPHY (MRCP) EXAMINATION

2019 ◽  
Vol 2 (3) ◽  
pp. 121
Author(s):  
Shinta J. Hapsari ◽  
Risalatul Latifah ◽  
Muhaimin Muhaimin
Keyword(s):  
Magnetic Resonance ◽  
Contrast Agents ◽  
Negative Contrast ◽  
Magnetic Resonance Cholangiopancreatography ◽  
Black Tea ◽  
Plain Water ◽  
Oral Contrast ◽  
Pineapple Juice ◽  
Long Time

Background: Magnetic resonance cholangiopancreatography examination is a superior examination in the detection of anatomic structures or pathological abnormalities in the human pancreatobiliary system. This examination is recommended to use negative oral contrast which aims to eliminate gastrointestinal signals that are around the pancreatobiliary system which can be a barrier to the visualization of the pancreatobiliary tract. Materials that can be used as oral contrast contrast must be able to survive for a long time in the gastrointestinal tract and contain elements of iron (Fe) and Manganese (Mn). The high Mn content of black and pineapple juice and its abundance need to be explored as contrast oral negative. Purpose: To prove the role of pineapple juice and black tea which are natural ingredients of negative oral contrast in the visualization of the pancreatobiliary system on MRCP examination. Methods: Experimental quasy studies have been carried out on 9 samples prospectively. Each sample underwent MRCP examination with three treatments, namely given black tea, pineapple juice and plain water. The image results from the three treatments were assessed by Radiology Doctors to assess the quality of their image. Results: The results showed that black tea was more effective in providing optimal image results on MRCP examination than pineapple juice and without using oral contrast contrast. The metal content of negative oral contrast can cause magnetic susceptibility which is characterized by the presence of shortening at the time of T2 relaxation. Negative contrast agents affect signal intensity usually by shortening T2 or T2 *, as an effect, areas of concern, especially areas with negative contrast agents will experience darkening. Conclusion: Black tea is effectively used as a negative oral contrat on MRCP examination.

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

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