scholarly journals Chondrogenic and BMP-4 primings confer osteogenesis potential to human cord blood mesenchymal stromal cells delivered with biphasic calcium phosphate ceramics

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Meadhbh Á. Brennan ◽  
Mario Barilani ◽  
Francesco Rusconi ◽  
Julien de Lima ◽  
Luciano Vidal ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Cord Blood ◽  
Stromal Cells ◽  
Biphasic Calcium Phosphate ◽  
Bone Repair ◽  
Calcium Phosphate Ceramics

AbstractBone marrow mesenchymal stem/stromal cells (BMSCs) show great promise for bone repair, however they are isolated by an invasive bone marrow harvest and their regenerative potential decreases with age. Conversely, cord blood can be collected non-invasively after birth and contains MSCs (CBMSCs) that can be stored for future use. However, whether CBMSCs can replace BMSCs targeting bone repair is unknown. This study evaluates the in vitro osteogenic potential of unprimed, osteogenically primed, or chondrogenically primed CBMSCs and BMSCs and their in vivo bone forming capacity following ectopic implantation on biphasic calcium phosphate ceramics in nude mice. In vitro, alkaline phosphatase (intracellular, extracellular, and gene expression), and secretion of osteogenic cytokines (osteoprotegerin and osteocalcin) was significantly higher in BMSCs compared with CBMSCs, while CBMSCs demonstrated superior chondrogenic differentiation and secretion of interleukins IL-6 and IL-8. BMSCs yielded significantly more cell engraftment and ectopic bone formation compared to CBMSCs. However, priming of CBMSCs with either chondrogenic or BMP-4 supplements led to bone formation by CBMSCs. This study is the first direct quantification of the bone forming abilities of BMSCs and CBMSCs in vivo and, while revealing the innate superiority of BMSCs for bone repair, it provides avenues to induce osteogenesis by CBMSCs.

Effects of in vitro chondrogenic priming time of bone-marrow-derived mesenchymal stromal cells on in vivo endochondral bone formation

2015 ◽  
Vol 13 ◽  
pp. 254-265 ◽  
Author(s):  
Wanxun Yang ◽  
Sanne K. Both ◽  
Gerjo J.V.M. van Osch ◽  
Yining Wang ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Endochondral Bone Formation ◽  
Endochondral Bone

Bone Marrow Autograft Associated to Macroporous Biphasic Calcium Phosphate for Bone Substitution in an Animal Model of Sequels of Radiotherapy

2005 ◽  
Vol 284-286 ◽  
pp. 285-288
Author(s):  
Oliver Malard ◽  
Jean Michel Bouler ◽  
Jerome Guicheux ◽  
Olivier Gauthier ◽  
E. Lerouxel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Biphasic Calcium Phosphate ◽  
Bone Repair ◽  
Major Surgery ◽  
Graft Versus Host Reaction ◽  
Bone Reconstruction ◽  
New Bone Formation ◽  
Study Results

Bone invasion is common in case of Squamous Cell Carcinomas (SCC) of the upper aero-digestive tract. Radiotherapy is required in addition to large surgical tumor removal. This treatment usually generates irreversible injuries on the reparation properties of the tissues, especially on bone. The quality of life of patients undergoing major surgery and radiotherapy in maxillary and mandible areas is reduced, but could be improved by bone reconstruction. The aim of this study was to evaluate the bone reconstruction possibilities by Macroporous Biphasic Calcium-Phosphate (MBCPÔ). The MBCP substitute was evaluated as granules and associated to autologous bone marrow (BM) graft in irradiated areas, in an inbreeding rodent model. Radiation sequels were created on inferior members of half of the rats. 3 weeks later, 3-mm osseous defects were created on each animal. The inbreeding model allows BM to be grafted without graft-versus-host reaction. Defects were filled either with MBCP alone, BM alone or a mixture of MBCP and BM. Six weeks after implantation, animals were sacrificed: bone repair and ceramic degradation were evaluated by qualitative and quantitative study. Results showed that bioceramics were well osteointegrated. Filling the defects with BM alone showed a significant increased of newly-formed bone formation but only after irradiation, whereas filling defects with MBCP alone increased new-bone formation only without previous irradiation. Associating MBCP to BM provided the best new-bone formation rates after irradiation. Degradation of the ceramic was the most important in case of BM grafting. This study demonstrated that BM added to MBCP constitute an appropriate material to be considered in case of bone defect occurring in irradiated tissue, and could be foreseen for use after bone removal for oncologic obligations.

scholarly journals In vitro and in vivo assessment of nanostructured porous biphasic calcium phosphate ceramics for promoting osteogenesis in an osteoporotic environment

RSC Advances ◽  
2018 ◽  
Vol 8 (26) ◽  
pp. 14646-14653 ◽  
Author(s):  
Kun Zhang ◽  
Jieyu Zhang ◽  
Kelei Chen ◽  
Xuefeng Hu ◽  
Yunbing Wang ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Defect ◽  
Biphasic Calcium Phosphate ◽  
Calcium Phosphate Ceramics ◽  
Defect Healing ◽  
Bone Defect Healing ◽  
In Vivo Assessment ◽  
Porous Biphasic Calcium Phosphate

Nanostructured porous biphasic calcium phosphate ceramics are able to significantly promote bone defect healing in an osteoporotic environment.

scholarly journals Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Chunrong Li ◽  
Xin Li ◽  
Xian Wang ◽  
Pei Miao ◽  
Jia Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Ovx Rats ◽  
Novel Target

Osteoporosis is a metabolic disease that results in the progressive loss of bone mass, which, in postmenopausal women, is related to oestrogen deficiency. Periostin (POSTN) plays a key role in the early stages of bone formation. However, whether POSTN participates in oestradiol regulation of osteogenic differentiation of bone marrow stromal cells (BMSCs) from ovariectomised (OVX) rats remains unclear. In vivo, using microcomputed tomography (micro-CT), immunohistochemistry, and dynamic analysis of femurs, we found that 17β-E2 promotes bone formation and POSTN expression at the endosteal surface. In vitro, 17β-E2 upregulated POSTN expression in OVX-BMSCs. POSTN overexpression activated the Wnt/β-catenin signalling pathway and enhanced osteogenic differentiation of OVX-BMSCs. Furthermore, knockdown of Postn blocks the involvement of 17β-E2 in the osteogenic differentiation of OVX-BMSCs. Collectively, our study indicated the role of POSTN in the osteogenesis and stemness of OVX-BMSCs and proves that 17β-E2 reduces osteoporosis and promotes osteogenesis through the POSTN-Wnt/β-catenin pathway. POSTN could, therefore, be a novel target gene for anti-osteoporosis therapies.

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