scholarly journals A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jantima Upan ◽  
Napaporn Youngvises ◽  
Adisorn Tuantranont ◽  
Chanpen Karuwan ◽  
Philippe Banet ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Platinum Nanoparticles ◽  
Alpha Fetoprotein ◽  
Label Free ◽  
Carbon Paste ◽  
Serum Samples ◽  
Redox Probe ◽  
Recognition Element ◽  
Before And After ◽  
Sensitive Determination

AbstractA label-free electrochemical aptamer-based sensor has been fabricated for alpha-fetoprotein (AFP) detection. Platinum nanoparticles on carboxylated-graphene oxide (PtNPs/GO-COOH) modified screen-printed graphene-carbon paste electrode (SPGE) was utilized as an immobilization platform, and the AFP aptamer was employed as a bio-recognition element. The synthesized GO-COOH helps to increase the surface area and amounts of the immobilized aptamer. Subsequently, PtNPs are decorated on GO-COOH to enhance electrical conductivity and an oxidation current of the hydroquinone electrochemical probe. The aptamer selectively interacts with AFP, causing a decrease in the peak current of the hydroquinone because the binding biomolecules on the electrode surface hinder the electron transfer of the redox probe. Effects of aptamer concentration and AFP incubation time were studied, and the current changes of the redox probe before and after AFP binding were investigated by square wave voltammetry. The developed aptasensor provides a linear range from 3.0–30 ng mL−1 with a detection limit of 1.22 ng mL−1. Moreover, the aptamer immobilized electrode offers high selectivity to AFP molecules, good stability, and sensitive determination of AFP in human serum samples with high recoveries.

A novel sensor for sensitive determination of atropine based on a Co3O4-reduced graphene oxide modified carbon paste electrode

2015 ◽  
Vol 39 (5) ◽  
pp. 3875-3881 ◽  
Author(s):  
Hasan Bagheri ◽  
Seyedeh Maryam Arab ◽  
Hosein Khoshsafar ◽  
Abbas Afkhami
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Carbon Paste Electrode ◽  
Electrochemical Determination ◽  
Modified Carbon Paste Electrode ◽  
Carbon Paste ◽  
Reduced Graphene ◽  
Sensitive Determination ◽  
Paste Electrode

Co3O4-reduced graphene oxide acted as a selective and sensitive modifier in a sensing layer for electrochemical determination of atropine.

A graphene oxide-based label-free electrochemical aptasensor for the detection of alpha-fetoprotein

2018 ◽  
Vol 112 ◽  
pp. 186-192 ◽  
Author(s):  
Shaohong Yang ◽  
Feifei Zhang ◽  
Zonghua Wang ◽  
Qionglin Liang
Keyword(s):  
Graphene Oxide ◽  
Alpha Fetoprotein ◽  
Label Free ◽  
Electrochemical Aptasensor

scholarly journals A Label-Free Immunosensor Based on Graphene Oxide/Fe3O4/Prussian Blue Nanocomposites for the Electrochemical Determination of HBsAg

Biosensors ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 24 ◽  
Author(s):  
Shanshan Wei ◽  
Haolin Xiao ◽  
Liangli Cao ◽  
Zhencheng Chen
Keyword(s):  
Graphene Oxide ◽  
Hepatitis B ◽  
Prussian Blue ◽  
Hepatitis B Surface Antigen ◽  
Electrochemical Determination ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Highly Sensitive

In this article, a highly sensitive label-free immunosensor based on a graphene oxide (GO)/Fe3O4/Prussian blue (PB) nanocomposite modified electrode was developed for the determination of human hepatitis B surface antigen (HBsAg). In this electrochemical immunoassay system, PB was used as a redox probe, while GO/Fe3O4/PB nanocomposites and AuNPs were prepared and coated on screen-printed electrodes to enhance the detection sensitivity and to immobilize the hepatitis B surface antibody (HBsAb). The immunosensor was fabricated based on the principle that the decrease in peak currents of PB is proportional to the concentration of HBsAg captured on the modified immunosensor. The experimental results revealed that the immunosensor exhibited a sensitive response to HBsAg in the range of 0.5 pg·mL−1 to 200 ng·mL−1, and with a low detection limit of 0.166 pg·mL−1 (S/N = 3). Furthermore, the proposed immunosensor was used to detect several clinical serum samples with acceptable results, and it also showed good reproducibility, selectivity and stability, which may have a promising potential application in clinical immunoassays.

Label-free electrochemical aptasensor based on reduced graphene oxide–hemin–chitosan nanocomposite for the determination of glypican-3

2021 ◽  
Author(s):  
Min Chen ◽  
Haimei Li ◽  
Xueming Su ◽  
Runqiang Wu ◽  
Huafu Feng ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Recognition Element ◽  
Reduced Graphene

A GPC3 electrochemical aptasensor was constructed using an RGO–H–CS-modified SPE as a biosensing platform and GPC3 aptamer as the recognition element.

scholarly journals A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin

2021 ◽  
Vol 11 (21) ◽  
pp. 10315
Author(s):  
Nang Noon Shean Aye ◽  
Pornsuda Maraming ◽  
Ratree Tavichakorntrakool ◽  
Attawut Chaibunruang ◽  
Patcharee Boonsiri ◽  
...  
Keyword(s):  
Glycated Albumin ◽  
Diabetic Patients ◽  
Label Free ◽  
Serum Samples ◽  
Covalent Interaction ◽  
Promising Tool ◽  
Before And After ◽  
Carboxylic Groups ◽  
Free Graphene ◽  
Specific Proteins

Glycated albumin (GA) has been previously introduced as a promising biomarker for glycemic monitoring in diabetes patients with thalassemia. In this study, a label-free graphene oxide (GO)-modified aptasensor was developed for the rapid detection of GA. The fabrication of the aptasensor was dependent on the covalent interaction of the amine-functionalized GA-specific aptamer with the carboxylic groups of GO. Square wave voltammetry (SWV) analysis was carried out for the measurement of GA-aptamer binding to their specific proteins. The peak current changes before and after incubation with GA protein were directly proportional to the concentration. The developed aptasensor exhibited a broad linearity (1–10,000 µg mL−1), a low detection limit (LOD) of 0.031 µg mL−1, and high selectivity for GA detection. In addition, the aptasensor was successfully applied to detect GA in both spiked and clinical serum samples. The comparison of the developed method with a commercial assay validated the reliability of the aptasensor for clinical application. Therefore, the newly developed aptasensor is a promising tool for GA measurements in diabetic patients with underlying thalassemia.

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