Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis

AbstractOdorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expression of AdisOBP genes in different tissues. We identified 23 OBPs in the testis and ovaries and 31 OBPs in antennal transcriptomes. The results of real-time quantitative PCR revealed that 23 of the 54 OBP genes were highly expressed in both female and male antennae, including three that exhibited male-biased expression and 15 that exhibited female-biased expression. A total of 24 OBPs were highly expressed in the testis of A. dissimilis, while expression of OBPs in the ovaries was very low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.

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Compared to Odorant-binding Proteins in the Reproductive System and Antennae of Athetis Dissimilis using Transcriptome Analysis

10.21203/rs.3.rs-199888/v1 ◽

2021 ◽

Author(s):

Yue-Qin Song ◽

Zhi-Yu Song ◽

Jun-Feng Dong ◽

Qi-Hui Lv ◽

Qing-Xiao Chen ◽

...

Keyword(s):

Quantitative Pcr ◽

Transcriptome Analysis ◽

Reproductive System ◽

Binding Proteins ◽

Reproductive Organs ◽

Odorant Binding Proteins ◽

Real Time Quantitative Pcr ◽

Lepidopteran Species ◽

Odorant Binding ◽

Different Tissues

Abstract Odorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expressive of OBPs in different tissues. We identified 23 OBPs in the testis and ovaries and 31 OBPs in antennal transcriptomes. The results of real-time quantitative PCR revealed that 23 of the 54 OBP genes were highly expressed in both female and male antennae, including three that exhibited male-biased expression and 15 that exhibited female-biased expression. A total of 24 OBPs were highly expressed in the testis of A. dissimilis, while expression of OBPs in the ovaries was very low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.

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Identification of odorant-binding proteins in the reproductive system of Athetis dissimilis using transcriptome analysis

10.21203/rs.2.19442/v1 ◽

2019 ◽

Author(s):

Yueqin Song ◽

Huizhong Sun ◽

Jingyun Zhao

Keyword(s):

Functional Diversity ◽

Transcriptome Analysis ◽

Reproductive System ◽

Binding Proteins ◽

Reproductive Organs ◽

Odorant Binding Proteins ◽

Chemosensory System ◽

Lepidopteran Species ◽

Odorant Binding ◽

Different Tissues

Abstract Odorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expressive of OBPs in different tissues. We identified a total of 54 OBPs including 23 OBPs in the transcriptomes of testis and ovaries, and 31 OBPs in antennal transcriptomes. Through fluorescence qPCR, the 23 identified OBPs were found to be highly expressed in both female and male antennae compared to the reproductive organs. Of the identified OBPs, 5/23 showed comparable expression in female and male antennae; 3/23 were more highly expressed in males compared to females; and 15/23 OBPs were more highly expressed in females compared to males. A total of 24 OBPs were highly expressed in the testis of A. dissimilis whilst expression in the ovaries was low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.

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The 40-Year Mystery of Insect Odorant-Binding Proteins

Biomolecules ◽

10.3390/biom11040509 ◽

2021 ◽

Vol 11 (4) ◽

pp. 509

Author(s):

Karen Rihani ◽

Jean-François Ferveur ◽

Loïc Briand

Keyword(s):

Binding Proteins ◽

Tissue Expression ◽

Reproductive Organs ◽

Odorant Binding Proteins ◽

Functional Roles ◽

Body Tissues ◽

Venom Glands ◽

Odorant Binding ◽

Chemosensory Organs ◽

Structural Aspects

The survival of insects depends on their ability to detect molecules present in their environment. Odorant-binding proteins (OBPs) form a family of proteins involved in chemoreception. While OBPs were initially found in olfactory appendages, recently these proteins were discovered in other chemosensory and non-chemosensory organs. OBPs can bind, solubilize and transport hydrophobic stimuli to chemoreceptors across the aqueous sensilla lymph. In addition to this broadly accepted “transporter role”, OBPs can also buffer sudden changes in odorant levels and are involved in hygro-reception. The physiological roles of OBPs expressed in other body tissues, such as mouthparts, pheromone glands, reproductive organs, digestive tract and venom glands, remain to be investigated. This review provides an updated panorama on the varied structural aspects, binding properties, tissue expression and functional roles of insect OBPs.

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