scholarly journals Compared to Odorant-binding Proteins in the Reproductive System and Antennae of Athetis Dissimilis using Transcriptome Analysis

2021 ◽  
Author(s):  
Yue-Qin Song ◽  
Zhi-Yu Song ◽  
Jun-Feng Dong ◽  
Qi-Hui Lv ◽  
Qing-Xiao Chen ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Transcriptome Analysis ◽  
Reproductive System ◽  
Binding Proteins ◽  
Reproductive Organs ◽  
Odorant Binding Proteins ◽  
Real Time Quantitative Pcr ◽  
Lepidopteran Species ◽  
Odorant Binding ◽  
Different Tissues

Abstract Odorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expressive of OBPs in different tissues. We identified 23 OBPs in the testis and ovaries and 31 OBPs in antennal transcriptomes. The results of real-time quantitative PCR revealed that 23 of the 54 OBP genes were highly expressed in both female and male antennae, including three that exhibited male-biased expression and 15 that exhibited female-biased expression. A total of 24 OBPs were highly expressed in the testis of A. dissimilis, while expression of OBPs in the ovaries was very low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.

scholarly journals Identification and comparative expression analysis of odorant-binding proteins in the reproductive system and antennae of Athetis dissimilis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yue-Qin Song ◽  
Zhi-Yu Song ◽  
Jun-Feng Dong ◽  
Qi-Hui Lv ◽  
Qing-Xiao Chen ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Reproductive System ◽  
Binding Proteins ◽  
Reproductive Organs ◽  
Odorant Binding Proteins ◽  
Real Time Quantitative Pcr ◽  
Lepidopteran Species ◽  
Comparative Expression Analysis ◽  
Odorant Binding ◽  
Different Tissues

AbstractOdorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expression of AdisOBP genes in different tissues. We identified 23 OBPs in the testis and ovaries and 31 OBPs in antennal transcriptomes. The results of real-time quantitative PCR revealed that 23 of the 54 OBP genes were highly expressed in both female and male antennae, including three that exhibited male-biased expression and 15 that exhibited female-biased expression. A total of 24 OBPs were highly expressed in the testis of A. dissimilis, while expression of OBPs in the ovaries was very low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.

scholarly journals Identification of odorant-binding proteins in the reproductive system of Athetis dissimilis using transcriptome analysis

2019 ◽  
Author(s):  
Yueqin Song ◽  
Huizhong Sun ◽  
Jingyun Zhao
Keyword(s):  
Functional Diversity ◽  
Transcriptome Analysis ◽  
Reproductive System ◽  
Binding Proteins ◽  
Reproductive Organs ◽  
Odorant Binding Proteins ◽  
Chemosensory System ◽  
Lepidopteran Species ◽  
Odorant Binding ◽  
Different Tissues

Abstract Odorant-binding proteins (OBPs) are prevalent in the antennal transcriptomes of different orders of insects. Studies on OBPs have focused on their role in the insect chemosensory system, but knowledge of their functions in the insect testis is limited. We sequenced the transcriptomes of the Athetis dissimilis reproductive organs and analyzed the expressive of OBPs in different tissues. We identified a total of 54 OBPs including 23 OBPs in the transcriptomes of testis and ovaries, and 31 OBPs in antennal transcriptomes. Through fluorescence qPCR, the 23 identified OBPs were found to be highly expressed in both female and male antennae compared to the reproductive organs. Of the identified OBPs, 5/23 showed comparable expression in female and male antennae; 3/23 were more highly expressed in males compared to females; and 15/23 OBPs were more highly expressed in females compared to males. A total of 24 OBPs were highly expressed in the testis of A. dissimilis whilst expression in the ovaries was low. These findings highlight the functional diversity of OBPs in insects and can facilitate further studies on the OBPs in A. dissimilis and lepidopteran species.

scholarly journals Antennal transcriptome analysis of olfactory genes and characterizations of odorant binding proteins in two woodwasps, Sirex noctilio and Sirex nitobei (Hymenoptera: Siricidae)

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Bing Guo ◽  
Enhua Hao ◽  
Haili Qiao ◽  
Jingzhen Wang ◽  
Weiwei Wu ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Native Species ◽  
Binding Proteins ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Species Differentiation ◽  
Sirex Noctilio ◽  
Odorant Binding Proteins ◽  
Species Specific ◽  
Odorant Binding

Abstract Background The woodwasp Sirex noctilio Fabricius is a major quarantine pest worldwide that was first discovered in China in 2013 and mainly harms Pinus sylvestris var. mongolica Litv.. S. nitobei Matsumura is a native species in China and is closely related to S. noctilio. Recently, the two woodwasps species were found attacking the P. sylvestris var. mongolica Litv in succession. The olfactory system is the foundation of insect behavior. Olfactory genes were identified through antennal transcriptome analysis. The expression profiles odorant binding proteins (OBPs) were analyzed with RT-qPCR. Results From our transcriptome analysis, 16 OBPs, 7 chemosensory proteins (CSPs), 41 odorant receptors (ORs), 8 gustatory receptors (GRs), 13 ionotropic receptors (IRs), and one sensory neuron membrane protein (SNMP) were identified in S. noctilio, while 15 OBPs, 6 CSPs, 43 ORs, 10 GRs, 16 IRs, and 1 SNMP were identified in S. nitobei. Most of the olfactory genes identified in two species were homologous. However, some species-specific olfactory genes were identified from the antennal transcriptomes, including SnocOBP13, SnocCSP6, SnocOR26, SnocGR2, SnocIR7 in S. noctilio and SnitGR9, SnitGR11, SnitIR17 in S. nitobei. In total, 14 OBPs were expressed primarily in the antennae. SnocOBP9 and SnitOBP9, identified as PBP homologues, were sex-biased expression in two siricid, but with different pattern. SnocOBP11 and SnitOBP11 were highly expressed in antennae and clearly expressed in external genitalia. SnocOBP7 and SnitOBP7 were highly expressed in male genitalia. SnocOBP3 and SnocOBP10 were highly expressed in female genitalia and male heads, while SnitOBP3 and SnitOBP10 did not show obvious tissue bias. Conclusion We analyzed 86 and 91 olfactory genes from S. noctilio and S. nitobei, respectively. Most of the olfactory genes identified were homologous, but also some species-specific olfactory genes were identified, which indicated the similarities and differences of the molecular mechanisms between the two closely-related species. Different expression in the antennae, external genitals or heads, exhibiting an obvious sex bias, suggested their different role in recognizing sex pheromones or plant volatiles. Species-specific expression for several OBPs genes may suggest that they strengthened or lost their original function during species differentiation, resulting in olfactory differences between the two species.

scholarly journals Antennal transcriptome analysis and expression profiles of odorant binding proteins in two woodwasps, Sirex noctilio and S. nitobei (Hymenoptera: Siricidae)

2019 ◽  
Author(s):  
Bing Guo ◽  
Jing-Zhen Wang ◽  
Hai-Li Qiao ◽  
Wei-Wei Wu ◽  
Jing-Jiang Zhou ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Olfactory System ◽  
Native Species ◽  
Binding Proteins ◽  
Expression Profiles ◽  
External Genitalia ◽  
Sirex Noctilio ◽  
Odorant Binding Proteins ◽  
Odor Recognition ◽  
Odorant Binding

Abstract Background The olfactory system is the foundation of insect behavior. Odorant binding proteins (OBPs) are key components of the insect olfactory system. The woodwasp Sirex noctilio Fabricius is a major quarantine pest worldwide that was first discovered in China in 2013 and mainly harms members of the Pinus genus. S. nitobei Matsumura is a native species in China and is closely related to S. noctilio. To gain insights into the olfactory mechanisms of these two woodwasp species, olfactory genes were identified using antennal transcriptome analysis. We also analyzed the expression profiles of OBPs with RT-qPCR. Results From our transcriptome analysis, 16 OBPs, 7 chemosensory proteins (CSPs), 41 odorant receptors (ORs), 8 gustatory receptors (GRs), 13 ionotropic receptors (IRs), and one sensory neuron membrane protein (SNMP) were identified in S. noctilio, while 15 OBPs, 6 CSPs, 43 ORs, 10 GRs, 16 IRs, and 1 SNMP were identified in S. nitobei. Most of the olfactory genes identified in two species were homologous. However, some species-specific olfactory genes were identified in the antennal transcriptomes, including SnocOBP13, SnocCSP6, SnocOR26, SnitGR9, and SnitIR17. In total, 14 OBPs (7 in S. noctilio and 7 in S. nitobei) were expressed primarily in the antennae of the two woodwasps. SnocOBP11 and SnitOBP11 were highly expressed in antennae and were also clearly expressed in the external genitalia. SnocOBP3 is highly expressed in the genitalia of females, and SnocOBP7 and SnitOBP7 are highly expressed in the genitalia of males. Meanwhile, SnocOBP10 was specifically expressed in male heads. Conclusion In total, 86 olfactory proteins were identified in S. noctilio, and 91 were identified in S. nitobei. Most SnocOBPs and SnitOBPs displayed enriched expression in the antennae, which are involved in odor recognition. A few OBPs were mainly expressed in the external genitals or heads and exhibited an obvious sex bias, which may indicate that the external genitals and heads are able to recognize sex pheromones or plant volatile compounds as a part of normal behaviors such as feeding, mating, or spawning. Our study provides key insights regarding the mechanism of interactions between the insect olfactory system and specific odor molecules.

scholarly journals The 40-Year Mystery of Insect Odorant-Binding Proteins

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 509
Author(s):  
Karen Rihani ◽  
Jean-François Ferveur ◽  
Loïc Briand
Keyword(s):  
Binding Proteins ◽  
Tissue Expression ◽  
Reproductive Organs ◽  
Odorant Binding Proteins ◽  
Functional Roles ◽  
Body Tissues ◽  
Venom Glands ◽  
Odorant Binding ◽  
Chemosensory Organs ◽  
Structural Aspects

The survival of insects depends on their ability to detect molecules present in their environment. Odorant-binding proteins (OBPs) form a family of proteins involved in chemoreception. While OBPs were initially found in olfactory appendages, recently these proteins were discovered in other chemosensory and non-chemosensory organs. OBPs can bind, solubilize and transport hydrophobic stimuli to chemoreceptors across the aqueous sensilla lymph. In addition to this broadly accepted “transporter role”, OBPs can also buffer sudden changes in odorant levels and are involved in hygro-reception. The physiological roles of OBPs expressed in other body tissues, such as mouthparts, pheromone glands, reproductive organs, digestive tract and venom glands, remain to be investigated. This review provides an updated panorama on the varied structural aspects, binding properties, tissue expression and functional roles of insect OBPs.

scholarly journals Antennal transcriptome analysis and expression profiles of odorant binding proteins in two woodwasps, Sirex noctilio and S. nitobei (Hymenoptera: Siricidae)

2019 ◽  
Author(s):  
Bing Guo ◽  
Jing-Zhen Wang ◽  
Hai-Li Qiao ◽  
Wei-Wei Wu ◽  
Jing-Jiang Zhou ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Olfactory System ◽  
Native Species ◽  
Binding Proteins ◽  
Expression Profiles ◽  
External Genitalia ◽  
Sirex Noctilio ◽  
Odorant Binding Proteins ◽  
Odor Recognition ◽  
Odorant Binding

Abstract Background The olfactory system is the foundation of insect behavior. Odorant binding proteins (OBPs) are key components of the insect olfactory system. The woodwasp Sirex noctilio Fabricius is a major quarantine pest worldwide that was first discovered in China in 2013 and mainly harms members of the Pinus genus. S. nitobei Matsumura is a native species in China and is closely related to S. noctilio. To gain insights into the olfactory mechanisms of these two woodwasp species, olfactory genes were identified using antennal transcriptome analysis. We also analyzed the expression profiles of OBPs with RT-qPCR. Results From our transcriptome analysis, 16 OBPs, 7 chemosensory proteins (CSPs), 41 odorant receptors (ORs), 8 gustatory receptors (GRs), 13 ionotropic receptors (IRs), and one sensory neuron membrane protein (SNMP) were identified in S. noctilio, while 15 OBPs, 6 CSPs, 43 ORs, 10 GRs, 16 IRs, and 1 SNMP were identified in S. nitobei. Most of the olfactory genes identified in two species were homologous. However, some species-specific olfactory genes were identified in the antennal transcriptomes, including SnocOBP13, SnocCSP6, SnocOR26, SnitGR9, and SnitIR17. In total, 14 OBPs (7 in S. noctilio and 7 in S. nitobei) were expressed primarily in the antennae of the two woodwasps. SnocOBP11 and SnitOBP11 were highly expressed in antennae and were also clearly expressed in the external genitalia. SnocOBP3 is highly expressed in the genitalia of females, and SnocOBP7 and SnitOBP7 are highly expressed in the genitalia of males. Meanwhile, SnocOBP10 was specifically expressed in male heads. Conclusion In total, 86 olfactory proteins were identified in S. noctilio, and 91 were identified in S. nitobei. Most SnocOBPs and SnitOBPs displayed enriched expression in the antennae, which are involved in odor recognition. A few OBPs were mainly expressed in the external genitals or heads and exhibited an obvious sex bias, which may indicate that the external genitals and heads are able to recognize sex pheromones or plant volatile compounds as a part of normal behaviors such as feeding, mating, or spawning. Our study provides key insights regarding the mechanism of interactions between the insect olfactory system and specific odor molecules.

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