Electro-spinning of highly-aligned polyacrylonitrile nano-fibres with continuous spooling

AbstractThis paper reports on a new configuration for producing highly-aligned electro-spun fibres that can be produced on a static substrate or one where it is hauled off and spooled continuously to enable the production of continuous lengths. The fixture consists of a Vee-shaped polytetrafluorethylene shield at 60° with a 1 cm wide integral rectangular base that is mounted on a copper disk with a 10 cm diameter. Specified concentrations of polyacrylonitrile in dimethyl sulfoxide were electro-spun on to a strip of cellulose paper. In the static setup, approximately 91% of the fibres were deposited to within 3°. When the spooling rig was used, a tape of the cellulose paper was hauled off at 0.07 mm/min, 78% of the fibres were aligned to within 3°. Simulations of the conventional and Vee-shield electro-spinning setups were undertaken and they provided corroboration for the experimental observations with regard to the mechanism responsible for fibre alignment. The feasibility of using this technique to produce 0°/− 45°/+ 45° stacked layers of aligned fibre preform is demonstrated.

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Dimethyl sulfoxide as a vehicle for corticosteroids. A comparison with the occlusive dressing technique

Archives of Dermatology ◽

10.1001/archderm.97.2.110 ◽

1968 ◽

Vol 97 (2) ◽

pp. 110-114 ◽

Cited By ~ 2

Author(s):

J. T. Groel

Keyword(s):

Dimethyl Sulfoxide ◽

Occlusive Dressing

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The Effect of Dimethyl Sulfoxide on In Vitro Platelet Function

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648027 ◽

1976 ◽

Vol 36 (01) ◽

pp. 221-229 ◽

Cited By ~ 16

Author(s):

Charles A. Schiffer ◽

Caroline L. Whitaker ◽

Morton Schmukler ◽

Joseph Aisner ◽

Steven L. Hilbert

Keyword(s):

Platelet Count ◽

Platelet Aggregation ◽

Dimethyl Sulfoxide ◽

Platelet Function ◽

Platelet Volume ◽

Short Term ◽

Platelet Factor ◽

Short Term Exposure ◽

Structural Abnormalities

SummaryAlthough dimethyl sulfoxide (DMSO) has been used extensively as a cryopreservative for platelets there are few studies dealing with the effect of DMSO on platelet function. Using techniques similar to those employed in platelet cryopreservation platelets were incubated with final concentrations of 2-10% DMSO at 25° C. After exposure to 5 and 10% DMSO platelets remained discoid and electron micrographs revealed no structural abnormalities. There was no significant change in platelet count. In terms of injury to platelet membranes, there was no increased availability of platelet factor-3 or leakage of nucleotides, 5 hydroxytryptamine (5HT) or glycosidases with final DMSO concentrations of 2.5, 5 and 10% DMSO. Thrombin stimulated nucleotide and 5HT release was reduced by 10% DMSO. Impairment of thrombin induced glycosidase release was noted at lower DMSO concentrations and was dose related. Similarly, aggregation to ADP was progressively impaired at DMSO concentrations from 1-5% and was dose related. After the platelets exposed to DMSO were washed, however, aggregation and release returned to control values. Platelet aggregation by epinephrine was also inhibited by DMSO and this could not be corrected by washing the platelets. DMSO-plasma solutions are hypertonic but only minimal increases in platelet volume (at 10% DMSO) could be detected. Shrinkage of platelets was seen with hypertonic solutions of sodium chloride or sucrose suggesting that the rapid transmembrane passage of DMSO prevented significant shifts of water. These studies demonstrate that there are minimal irreversible alterations in in vitro platelet function after short-term exposure to DMSO.

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Preservation of Morphological Integrity and Clot Retraction Activity of Human Platelets After Freezing

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654822 ◽

1964 ◽

Vol 11 (01) ◽

pp. 222-229 ◽

Cited By ~ 8

Author(s):

Isaac Djerassi ◽

Albert Roy ◽

Jorge Alvarado ◽

Keyword(s):

Dimethyl Sulfoxide ◽

Liquid Nitrogen ◽

Human Platelets ◽

Slow Freezing ◽

Critical Conditions ◽

Plasma Medium ◽

Clot Retraction ◽

Controlled Freezing ◽

Direct Immersion

SummaryHuman platelets frozen at −195° C (liquid nitrogen) retain their morphological integrity and ability to promote clot retraction when 5% dimethyl-sulfoxide and 5% dextrose are added to the suspending plasma medium. Slow freezing was more effective than direct immersion in the liquid nitrogen. Although similar results may be achieved with dimethylsulfoxide alone with rigidly controlled freezing rates, the addition of sugars may permit freezing under less critical conditions.Dimethylsulfoxyd und 5% Dextrose dem Plasmamilieu hinzugefügt werden. Das langsame Einfrieren ist effektiver als das direkte Eintauchen in flüssigen Stickstoff. Obschon ähnliche Resultate mit Dimethylsulfoxyd allein unter exakter Kontrolle der Einfrierungsgeschwindig-keit erreicht werden können, erlaubt die Zugabe von Dextrose ein Einfrieren unter weniger kritischen Bedingungen.

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1700589: Mechanistic aspects of ligand substitution on the cis-diaqua-chloro-tris(dimethyl sulfoxide)ruthenium(II) complex by some sulfur-containing bioactive ligands in aqueous medium-ESI

Progress in Reaction Kinetics and Mechanism ◽

10.3184/146867818x15319903829254 ◽

2018 ◽

Keyword(s):

Dimethyl Sulfoxide ◽

Aqueous Medium ◽

Ligand Substitution ◽

Bioactive Ligands ◽

Sulfur Containing

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Substance Overview for Dimethyl sulfoxide

The MAK-Collection for Occupational Health and Safety ◽

10.1002/3527600418.mbe6768 ◽

2014 ◽

pp. 1-1

Keyword(s):

Dimethyl Sulfoxide

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Viscosity of Dimethyl Sulfoxide + 1,4 Dimethylbenzene System

Revista de Chimie ◽

10.37358/rc.08.1.1704 ◽

2008 ◽

Vol 59 (1) ◽

pp. 45-48

Author(s):

Oana Ciocirlan ◽

Olga Iulian

Keyword(s):

Free Energy ◽

Dimethyl Sulfoxide ◽

Atmospheric Pressure ◽

Entire Range ◽

Polynomial Equation ◽

Energy Of Activation ◽

Excess Gibbs Free Energy ◽

Experimental Measurements ◽

Gibbs Energy Of Activation ◽

Free Energy Of Activation

This paper reports the viscosities measurements for the binary system dimethyl sulfoxide + 1,4-dimethylbenzene over the entire range of mole fraction at 298.15, 303.15, 313.15 and 323.15 K and atmospheric pressure. The experimental viscosities were correlated with the equations of Grunberg-Nissan, Katti-Chaudhri, Hind, Soliman and McAllister; the adjustable binary parameters have been obtained. The excess Gibbs energy of activation of viscous flow (G*E) has been calculated from the experimental measurements and the results were fitted to Redlich-Kister polynomial equation. The obtained negative excess Gibbs free energy of activation and negative Grunberg-Nissan interaction parameter are discussed in structural and interactional terms.

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Dimethyl-sulfoxide is a Suitable Solvent for Fluorescent Microscopy Detection of Medium and Strong Heat Shock Inductors Using Transgenic Zebrafish

Revista de Chimie ◽

10.37358/rc.18.2.6102 ◽

2018 ◽

Vol 69 (2) ◽

pp. 337-340

Author(s):

Vlad Preluca ◽

Bogdan Horatiu Serb ◽

Sanda Marchian ◽

Diter Atasie ◽

Mihaela Cernusca Mitariu ◽

...

Keyword(s):

Heat Shock ◽

Dimethyl Sulfoxide ◽

Heat Shock Response ◽

Protein Misfolding ◽

Fluorescent Microscopy ◽

Screening Tests ◽

Transgenic Zebrafish ◽

Fluorescent Intensity ◽

Shock Response ◽

Heat Shock Induction

Heat shock inductors have potential as treatment for degenerative and protein misfolding diseases. Dimethyl-sulfoxide is widely used as a solvent in pharmacological screening tests and has been shown to have heat shock induction effects. Transgenic Tg (hsp70l:EGFP-HRAS_G12V)io3(AB) zebrafish larvae were exposed for 24 hours to dimethyl-sulfoxide in concentratios of 0.1-2%, and to moderate heat shock inductors pentoxifylline and tacrolimus. Positive controls were exposed to 35, 38 and 40�C for 20 min, and incubated for 24 h at 28�C. Heat shock response was measured by fluorescence microscopy and signal intensity quantification in FIJI. Dimethyl-sulfoxide caused a dose-dependant increase in fluorescent intensity, but significantly lower compared with exposure to 38 and 40�C. Pentoxifylline and tacrolimus induced a significantly higher increase in fluorescence compared with 0.5% dimethyl-sulfoxide. Thus, although dimethyl-sulfoxide has independent heat shock induction effects, concentrations of up to 0.5% are suitable for heat shock response screening tests.

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