scholarly journals Publisher Correction: Single-cell mapping of DNA G-quadruplex structures in human cancer cells

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Winnie W. I. Hui ◽  
Angela Simeone ◽  
Katherine G. Zyner ◽  
David Tannahill ◽  
Shankar Balasubramanian
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Human Cancer ◽  
Cell Mapping ◽  
Human Cancer Cells ◽  
G Quadruplex

scholarly journals Monohydrazone Based G-Quadruplex Selective Ligands Induce DNA Damage and Genome Instability in Human Cancer Cells

2020 ◽  
Vol 63 (6) ◽  
pp. 3090-3103 ◽  
Author(s):  
Jussara Amato ◽  
Giulia Miglietta ◽  
Rita Morigi ◽  
Nunzia Iaccarino ◽  
Alessandra Locatelli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Genome Instability ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
G Quadruplex ◽  
Selective Ligands

Abstract 4838: R loop-driven genome instability by G-quadruplex binders in BRCA2-silenced human cancer cells

2018 ◽  
Author(s):  
Alessio De Magis ◽  
Stefano Giustino Manzo ◽  
Marco Russo ◽  
Olivier Sordet ◽  
Rita Morigi ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Genome Instability ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
G Quadruplex

scholarly journals Targeting G-quadruplex DNA with synthetic dendritic peptide: modulation of the proliferation of human cancer cells

RSC Advances ◽  
2020 ◽  
Vol 10 (44) ◽  
pp. 26388-26396
Author(s):  
Soumi Biswas ◽  
Satyabrata Samui ◽  
Apurba K. Das ◽  
Sanjeev Pasadi ◽  
K. Muniyappa ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Quadruplex Dna ◽  
Human Cancer Cells ◽  
G4 Dna ◽  
G Quadruplex ◽  
Peptide Targeting

A synthetic dendritic peptide, targeting human telomeric G4 DNA, inhibits the telomerase and lessens the proliferation of human cancer cells.

scholarly journals A Nucleus-Imaging Probe That Selectively Stabilizes a Minor Conformation of c-MYC G-quadruplex and Down-regulates c-MYC Transcription in Human Cancer Cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Deepanjan Panda ◽  
Manish Debnath ◽  
Samir Mandal ◽  
Irene Bessi ◽  
Harald Schwalbe ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Imaging Probe ◽  
Human Cancer Cells ◽  
G Quadruplex ◽  
A Minor

scholarly journals Selective recognition of c-myc promoter G-quadruplex and down-regulation of oncogene c-myc transcription in human cancer cells by 3,8a-disubstituted indolizinone

RSC Advances ◽  
2017 ◽  
Vol 7 (82) ◽  
pp. 51965-51969 ◽  
Author(s):  
Fengmin Yang ◽  
Xin Sun ◽  
Lixia Wang ◽  
Qian Li ◽  
Aijiao Guan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Selective Recognition ◽  
Down Regulation ◽  
Human Cancer Cells ◽  
G Quadruplex

Indolizinone could selectively recognize c-myc promoter G-quadruplex.

scholarly journals Single-cell imaging reveals unexpected heterogeneity of TERT expression across cancer cell lines

2019 ◽  
Author(s):  
Teisha J. Rowland ◽  
Gabrijela Dumbović ◽  
Evan P. Hass ◽  
John L. Rinn ◽  
Thomas R. Cech
Keyword(s):  
Single Cell ◽  
Cancer Cells ◽  
Single Molecule ◽  
Human Cancer ◽  
Expression Patterns ◽  
Mrna Levels ◽  
Single Molecule Level ◽  
Human Cancer Cells ◽  
Transcription Sites ◽  
Tert Expression

AbstractTelomerase is pathologically reactivated in most human cancers, where it maintains chromosomal telomeres and allows immortalization. Because telomerase reverse transcriptase (TERT) is usually the limiting component for telomerase activation, numerous studies have measured TERT mRNA levels in populations of cells or in tissues. However, little is known about TERT expression at the single-cell and single-molecule level. Here we analyzed TERT expression across 10 human cancer lines using single-molecule RNA FISH and made several unexpected findings. First, there was substantial cell-to-cell variation in number of transcription sites and ratio of transcription sites to gene copies. Second, previous classification of lines as having monoallelic or biallelic TERT expression was found to be inadequate for capturing the TERT gene expression patterns. Finally, TERT mRNA had primarily nuclear localization in cancer cells and induced pluripotent stem cells (iPSCs), in stark contrast to the expectation that mature mRNA should be predominantly cytoplasmic. These data reveal unappreciated heterogeneity, complexity, and unconventionality in TERT expression across human cancer cells.

scholarly journals DNA damage and genome instability by G-quadruplex ligands are mediated by R loops in human cancer cells

2018 ◽  
Vol 116 (3) ◽  
pp. 816-825 ◽  
Author(s):  
Alessio De Magis ◽  
Stefano G. Manzo ◽  
Marco Russo ◽  
Jessica Marinello ◽  
Rita Morigi ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Genome Instability ◽  
Human Cancer ◽  
Cell Killing ◽  
Dna Structures ◽  
Human Cancer Cells ◽  
Cellular Effects ◽  
Genome Wide ◽  
G Quadruplex

G quadruplexes (G4s) and R loops are noncanonical DNA structures that can regulate basic nuclear processes and trigger DNA damage, genome instability, and cell killing. By different technical approaches, we here establish that specific G4 ligands stabilize G4s and simultaneously increase R-loop levels within minutes in human cancer cells. Genome-wide mapping of R loops showed that the studied G4 ligands likely cause the spreading of R loops to adjacent regions containing G4 structures, preferentially at 3′-end regions of expressed genes, which are partially ligand-specific. Overexpression of an exogenous human RNaseH1 rescued DNA damage induced by G4 ligands in BRCA2-proficient and BRCA2-silenced cancer cells. Moreover, even if the studied G4 ligands increased noncanonical DNA structures at similar levels in nuclear chromatin, their cellular effects were different in relation to cell-killing activity and stimulation of micronuclei, a hallmark of genome instability. Our findings therefore establish that G4 ligands can induce DNA damage by an R loop-dependent mechanism that can eventually lead to different cellular consequences depending on the chemical nature of the ligands.

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