scholarly journals Cell fate-specific regulation of EGF receptor trafficking during Caenorhabditis elegans vulval development

2006 ◽  
Vol 25 (11) ◽  
pp. 2347-2357 ◽  
Author(s):  
Attila Stetak ◽  
Erika Fröhli Hoier ◽  
Assunta Croce ◽  
Giuseppe Cassata ◽  
Pier Paolo Di Fiore ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Egf Receptor ◽  
Receptor Trafficking ◽  
Vulval Development ◽  
Specific Regulation

scholarly journals The CHORD protein CHP-1 regulates EGF receptor trafficking and signaling in C. elegans and in human cells

2019 ◽  
Author(s):  
Andrea Haag ◽  
Michael Walser ◽  
Adrian Henggeler ◽  
Alex Hajnal
Keyword(s):  
Intracellular Trafficking ◽  
Egf Receptor ◽  
Mapk Signaling ◽  
Human Cells ◽  
Receptor Trafficking ◽  
Independent Function ◽  
Vulval Development ◽  
C Elegans ◽  
Downstream Signaling

AbstractThe intracellular trafficking of growth factor receptors determines the activity of their downstream signaling pathways. The putative co-chaperone CHP-1 acts as a regulator of EGFR trafficking during C.elegans vulval development. Loss of chp-1 causes the retention of the EGFR in the ER and decreased MAPK signaling. CHP-1 functions specifically, as the localization of other receptors is unaltered in chp-1(lf) mutants, and inhibiting other co-chaperones does not affect EGFR localization. The role of CHP-1 during EGFR trafficking is conserved in humans. Analogous to C.elegans, the response of CHP-1-deficient human cells to EGF stimulation is attenuated, the EGFR accumulates in the ER and ERK2 activity is decreased. Although CHP-1 has been proposed to act as a co-chaperone for HSP90, our data indicate an HSP90-independent function of CHP-1. The identification of CHP-1 as a regulator of EGFR trafficking opens the possibility to identify small molecule chaperone inhibitors targeting the EGFR pathway with increased selectivity.

Reversal of cell fate determination in Caenorhabditis elegans vulval development

Development ◽  
1996 ◽  
Vol 122 (8) ◽  
pp. 2507-2515 ◽  
Author(s):  
S. Euling ◽  
V. Ambros
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Larval Stage ◽  
Cell Types ◽  
Wild Type ◽  
Vulval Development ◽  
Fate Determination ◽  
Developmental Arrest ◽  
Dauer Larva ◽  
Vulval Precursor Cells

In Caenorhabditis elegans, the fates of the multipotent vulval precursor cells (VPCs) are specified by intercellular signals. The VPCs divide in the third larval stage (L3) of the wild type, producing progeny of determined cell types. In lin-28 mutants, vulva development is similar to wild-type vulva development except that it occurs precociously, in the second larval stage (L2). Consequently, when lin-28 hermaphrodites temporarily arrest development at the end of L2 in the dauer larva stage, they have partially developed vulvae consisting of VPC progeny. During post-dauer development, these otherwise determined VPC progeny become reprogrammed back to the multipotent, signal-sensitive state of VPCs. Our results indicate that VPC fate determination by intercellular signals is reversible by dauer larva developmental arrest and post-dauer development.

scholarly journals Reciprocal EGFR signaling in the anchor cell ensures precise inter-organ connection during Caenorhabditis elegans vulval morphogenesis

Development ◽  
2022 ◽  
Vol 149 (1) ◽  
Author(s):  
Silvan Spiri ◽  
Simon Berger ◽  
Louisa Mereu ◽  
Andrew DeMello ◽  
Alex Hajnal
Keyword(s):  
Caenorhabditis Elegans ◽  
Egf Receptor ◽  
Adherens Junctions ◽  
Egfr Signaling ◽  
Cell Fates ◽  
Vulval Development ◽  
Sequence Of Events ◽  
Epidermal Growth ◽  
Short And Long Term ◽  
Anchor Cell

ABSTRACT During Caenorhabditis elegans vulval development, the uterine anchor cell (AC) first secretes an epidermal growth factor (EGF) to specify the vulval cell fates and then invades the underlying vulval epithelium. By doing so, the AC establishes direct contact with the invaginating primary vulF cells and attaches the developing uterus to the vulva. The signals involved and the exact sequence of events joining these two organs are not fully understood. Using a conditional let-23 EGF receptor (EGFR) allele along with novel microfluidic short- and long-term imaging methods, we discovered a specific function of the EGFR in the AC during vulval lumen morphogenesis. Tissue-specific inactivation of let-23 in the AC resulted in imprecise alignment of the AC with the primary vulval cells, delayed AC invasion and disorganized adherens junctions at the contact site forming between the AC and the dorsal vulF toroid. We propose that EGFR signaling, activated by a reciprocal EGF cue from the primary vulval cells, positions the AC at the vulval midline, guides it during invasion and assembles a cytoskeletal scaffold organizing the adherens junctions that connect the developing uterus to the dorsal vulF toroid. Thus, EGFR signaling in the AC ensures the precise alignment of the two developing organs.

Caenorhabditis elegans lin-25: A Study of Its Role in Multiple Cell Fate Specification Events Involving Ras and the Identification and Characterization of Evolutionarily Conserved Domains

Genetics ◽  
2000 ◽  
Vol 156 (3) ◽  
pp. 1083-1096
Author(s):  
Lars Nilsson ◽  
Teresa Tiensuu ◽  
Simon Tuck
Keyword(s):  
Caenorhabditis Elegans ◽  
Cell Fate ◽  
Nuclear Translocation ◽  
Cell Fate Specification ◽  
Sequence Comparisons ◽  
Vulval Development ◽  
Fate Specification ◽  
C Elegans

Abstract Caenorhabditis elegans lin-25 functions downstream of let-60 ras in the genetic pathway for the induction of the 1° cell fate during vulval development and encodes a novel 130-kD protein. The biochemical activity of LIN-25 is presently unknown, but the protein appears to function together with SUR-2, whose human homologue binds to Mediator, a protein complex required for transcriptional regulation. We describe here experiments that indicate that, besides its role in vulval development, lin-25 also participates in the fate specification of a number of other cells in the worm that are known to require Ras-mediated signaling. We also describe the cloning of a lin-25 orthologue from C. briggsae. Sequence comparisons suggest that the gene is evolving relatively rapidly. By characterizing the molecular lesions associated with 10 lin-25 mutant alleles and by assaying in vivo the activity of mutants lin-25 generated in vitro, we have identified three domains within LIN-25 that are required for activity or stability. We have also identified a sequence that is required for efficient nuclear translocation. We discuss how lin-25 might act in cell fate specification in C. elegans within the context of models for lin-25 function in cell identity and cell signaling.

scholarly journals Transcriptional network underlying Caenorhabditis elegans vulval development

2005 ◽  
Vol 102 (14) ◽  
pp. 4972-4977 ◽  
Author(s):  
T. Inoue ◽  
M. Wang ◽  
T. O. Ririe ◽  
J. S. Fernandes ◽  
P. W. Sternberg
Keyword(s):  
Caenorhabditis Elegans ◽  
Transcriptional Network ◽  
Vulval Development
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