scholarly journals Study of proinflammatory responses induced by Yersinia pestis in human monocytes using cDNA arrays

2007 ◽  
Vol 8 (4) ◽  
pp. 308-319 ◽  
Author(s):  
R Das ◽  
A Dhokalia ◽  
X-Z Huang ◽  
R Hammamieh ◽  
N Chakraborty ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Human Monocytes ◽  
Cdna Arrays ◽  
Proinflammatory Responses

scholarly journals Hyperglycemic Memory of Innate Immune Cells Promotes In Vitro Proinflammatory Responses of Human Monocytes and Murine Macrophages

2021 ◽  
pp. ji1901348
Author(s):  
Kathrin Thiem ◽  
Samuel T. Keating ◽  
Mihai G. Netea ◽  
Niels P. Riksen ◽  
Cees J. Tack ◽  
...  
Keyword(s):  
Immune Cells ◽  
Innate Immune ◽  
Innate Immune Cells ◽  
Human Monocytes ◽  
Murine Macrophages ◽  
Proinflammatory Responses

scholarly journals Biochemical and Functional Analysis of Cyanobacterium Geitlerinema sp. LPS on Human Monocytes

2019 ◽  
Vol 171 (2) ◽  
pp. 421-430 ◽  
Author(s):  
Michelle Swanson-Mungerson ◽  
Philip G Williams ◽  
Joshua R Gurr ◽  
Ryan Incrocci ◽  
Vijay Subramaniam ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid A ◽  
Positive Control ◽  
Cyanobacterial Blooms ◽  
Side Chains ◽  
Human Monocytes ◽  
E Coli ◽  
Proinflammatory Responses ◽  
The Difference ◽  
High Concentrations

Abstract Cyanobacterial blooms are an increasing source of environmental toxins that affect both human and animals. After ingestion of cyanobacteria, such as Geitlerinema sp., toxins and lipopolysaccharide (LPS) from this organism induce fever, gastrointestinal illness, and even death. However, little is known regarding the effects of cyanobacterial LPS on human monocytes after exposure to LPS upon ingestion. Based on our previous data using Geitlerinema sp. LPS (which was previously named Oscillatoria sp., a genus belonging to the same order as Geitlerinema), we hypothesized that Geitlerinema sp. LPS would activate human monocytes to proliferate, phagocytose particles, and produce cytokines that are critical for promoting proinflammatory responses in the gut. Our data demonstrate that Geitlerinema sp. LPS induced monocyte proliferation and TNF-α, IL-1, and IL-6 production at high concentrations. In contrast, Geitlerinema sp. LPS is equally capable of inducing monocyte-mediated phagocytosis of FITC-latex beads when compared with Escherichia coli LPS, which was used as a positive control for our experiments. In order to understand the mechanism responsible for the difference in efficacy between Geitlerinema sp. LPS and E. coli LPS, we performed biochemical analysis and identified that Geitlerinema sp. LPS was composed of significantly different sugars and fatty acid side chains in comparison to E. coli LPS. The lipid A portion of Geitlerinema sp. LPS contained longer fatty acid side chains, such as C15:0, C16:0, and C18:0, instead of C12:0 found in E. coli LPS which may explain the decreased efficacy and toxicity of Geitlerinema sp. LPS in comparison to E. coli LPS.

scholarly journals Ligation of the FcRγ Chain-Associated Human Osteoclast-Associated Receptor Enhances the Proinflammatory Responses of Human Monocytes and Neutrophils

2006 ◽  
Vol 176 (5) ◽  
pp. 3149-3156 ◽  
Author(s):  
Estelle Merck ◽  
Claude Gaillard ◽  
Mathieu Scuiller ◽  
Patrizia Scapini ◽  
Marco A. Cassatella ◽  
...  
Keyword(s):  
Human Monocytes ◽  
Proinflammatory Responses ◽  
Human Osteoclast

Ligation of the BT3 molecules, members of the B7 family, enhance the proinflammatory responses of human monocytes and monocyte-derived dendritic cells

2010 ◽  
Vol 48 (1-3) ◽  
pp. 109-118 ◽  
Author(s):  
Rita Simone ◽  
Bernadette Barbarat ◽  
Andrea Rabellino ◽  
Giancarlo Icardi ◽  
Marcello Bagnasco ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Human Monocytes ◽  
B7 Family ◽  
Proinflammatory Responses

scholarly journals The Yersinia pestis Ail Protein Mediates Binding and Yop Delivery to Host Cells Required for Plague Virulence

2008 ◽  
Vol 77 (2) ◽  
pp. 825-836 ◽  
Author(s):  
Suleyman Felek ◽  
Eric S. Krukonis
Keyword(s):  
Yersinia Pestis ◽  
Type Iii Secretion ◽  
Parental Strain ◽  
Binding Capacity ◽  
Lethal Dose ◽  
Host Cells ◽  
Human Monocytes ◽  
Cell Binding ◽  
Single Deletion

ABSTRACT Although adhesion to host cells is a critical step in the delivery of cytotoxic Yop proteins by Yersinia pestis, the mechanism has not been defined. To identify adhesins critical for Yop delivery, we initiated two transposon mutagenesis screens using the mariner transposon. To avoid redundant cell binding activities, we initiated the screen with a strain deleted for two known adhesins, pH 6 antigen and the autotransporter, YapC, as well as the Caf1 capsule, which is known to obscure some adhesins. The mutants that emerged contained insertions within the ail (attachment and invasion locus) gene of Y. pestis. A reconstructed mutant with a single deletion in the ail locus (y1324) was severely defective for delivery of Yops to HEp-2 human epithelial cells and significantly defective for delivery of Yops to THP-1 human monocytes. Specifically, the Yop delivery defect was apparent when cell rounding and translocation of an ELK-tagged YopE derivative into host cells were monitored. Although the ail mutant showed only a modest decrease in cell binding capacity in vitro, the KIM5 Δail mutant exhibited a >3,000-fold-increased 50% lethal dose in mice. Mice infected with the Δail mutant also had 1,000-fold fewer bacteria in their spleens, livers, and lungs 3 days after infection than did those infected with the parental strain, KIM5. Thus, the Ail protein is critical for both Y. pestis type III secretion in vitro and infection in mice.

scholarly journals Rapid Induction of Autophagy by Infection of Human Monocytes with Yersinia pestis

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Jean‐Nicolas Tournier ◽  
Scott Adrian ◽  
Ramin Hakami ◽  
Gordon Ruthel ◽  
Robert G Ulrich
Keyword(s):  
Yersinia Pestis ◽  
Human Monocytes ◽  
Rapid Induction

Ultrastructural observations of human monocytes infected with HIV-1

1991 ◽  
Vol 49 ◽  
pp. 108-109
Author(s):  
James K. Koehler ◽  
Steven G. Reed ◽  
Joao S. Silva
Keyword(s):  
Gradient Centrifugation ◽  
Virus Production ◽  
Human Monocyte ◽  
Red Cross ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
Hiv Replication ◽  
Electron Microscopic ◽  
Ultrastructural Studies ◽  
Hiv 1

As part of a larger study involving the co-infection of human monocyte cultures with HIV and protozoan parasites, electron microscopic observations were made on the course of HIV replication and infection in these cells. Although several ultrastructural studies of the cytopathology associated with HIV infection have appeared, few studies have shown the details of virus production in “normal,” human monocytes/macrophages, one of the natural targets of the virus, and suspected of being a locus of quiescent virus during its long latent period. In this report, we detail some of the interactions of developing virons with the membranes and organelles of the monocyte host.Peripheral blood monocytes were prepared from buffy coats (Portland Red Cross) by Percoll gradient centrifugation, followed by adherence to cover slips. 90-95% pure monocytes were cultured in RPMI with 5% non-activated human AB serum for four days and infected with 100 TCID50/ml of HIV-1 for four hours, washed and incubated in fresh medium for 14 days.

Distribution of VLA-5 and VLA-4 fibronectin receptors in human monocytes demonstrated by immunofluorescence, immunocytochemistry, and autoradiography

1993 ◽  
Vol 51 ◽  
pp. 306-307
Author(s):  
Robert Williams ◽  
Che-Hung Lee ◽  
Sara E. Quella ◽  
David M. Harlan ◽  
Yuan-Hsu Kang
Keyword(s):  
Present Report ◽  
Matrix Proteins ◽  
Extracellular Matrices ◽  
Human Monocytes ◽  
Integrin Receptors ◽  
Morphological Evaluation ◽  
The Matrix ◽  
Specific Receptors ◽  
Cytokine Stimulation ◽  
Monocyte Adherence

Monocyte adherence to endothelial or extracellular matrices plays an important role in triggering monocyte activation in extravascular sites of infection, chronic inflammatory disorders, and tissue damage. Migration of monocytes in the tissues involves the response to a chemoattractant and movement by a series of attachments and detachments to the extracellular matrices which are regulated by expression and distribution of specific receptors for the matrix proteins such as fibronectin (FN). The VSAs (very late antigens or beta integrins), a subfamily of the transmembrane heterodimeric integrin receptors, have been thought to play a major role in monocyte adherence to the extracellular matrices and cells. In this subfamily, VLA-5 and VLA-4 are believed to be the most essential integrins mediating monocyte adherence to FN. In the present report, we have established and compared different procedures for morphological evaluation of the expression and distribution of the FN receptors on human monocytes in order to investigate their response to endotoxin or cytokine stimulation.

Sign in / Sign up

Export Citation Format

Share Document