scholarly journals In vivo cell type-specific gene delivery with retroviral vectors that display single chain antibodies

Gene Therapy ◽  
1999 ◽  
Vol 6 (12) ◽  
pp. 1982-1987 ◽  
Author(s):  
A Jiang ◽  
R Dornburg ◽  
R Dornburg
Keyword(s):  
Gene Delivery ◽  
Retroviral Vectors ◽  
Specific Gene ◽  
Cell Type ◽  
Single Chain ◽  
Single Chain Antibodies ◽  
Cell Type Specific

scholarly journals Cell-Type-Specific Gene Transfer into Human Cells with Retroviral Vectors That Display Single-Chain Antibodies

1998 ◽  
Vol 72 (12) ◽  
pp. 10148-10156 ◽  
Author(s):  
An Jiang ◽  
Te-Hua T. Chu ◽  
F. Nocken ◽  
Klaus Cichutek ◽  
Ralph Dornburg
Keyword(s):  
Gene Delivery ◽  
Cell Surface ◽  
Retroviral Vectors ◽  
Human Cells ◽  
Specific Gene ◽  
Cell Type ◽  
Single Chain ◽  
Single Chain Antibodies ◽  
Cell Type Specific ◽  
Vector Particles

ABSTRACT The successful application of human gene therapy protocols on a broad clinical basis will depend on the availability of in vivo cell-type-specific gene delivery systems. We have developed retroviral vector particles, derived from spleen necrosis virus (SNV), that display the antigen binding site of an antibody on the viral surface. Using retroviral vectors derived from SNV that displayed single-chain antibodies (scAs) directed against a carcinoembryonic antigen-cross-reacting cell surface protein, we have shown that an efficient, cell-type-specific gene delivery can be obtained. In this study, we tested whether other scAs displayed on SNV vector particles can also lead to cell-type-specific gene delivery. We displayed the following scAs on the retroviral surface: one directed against the human cell surface antigen Her2neu, which belongs to the epidermal growth factor receptor family; one directed against the stem cell-specific antigen CD34; and one directed against the transferrin receptor, which is expressed on liver cells and various other tissues. We show that retroviral vectors displaying these scAs are competent for infection in human cells which express the antigen recognized by the scA. Infectivity was cell type specific, and titers above 105 CFU per ml of tissue culture supernatant medium were obtained. The density of the antigen on the target cell surface does not influence virus titers in vitro. Our data indicate that the SNV vector system is well suited for the development of a large variety of cell-type-specific targeting vectors.

scholarly journals Cell-type-specific gene delivery into neuronal cells in vitro and in vivo

Virology ◽  
2003 ◽  
Vol 314 (1) ◽  
pp. 74-83 ◽  
Author(s):  
Zahida Parveen ◽  
Muhammad Mukhtar ◽  
Mohammed Rafi ◽  
David A Wenger ◽  
Khwaja M Siddiqui ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Neuronal Cells ◽  
Specific Gene ◽  
Cell Type ◽  
Cell Type Specific

scholarly journals Cell-Type Specific Expression of the Vasopressin Gene Analyzed by AAV Mediated Gene Delivery of Promoter Deletion Constructs into the Rat SON In Vivo

PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e48860 ◽  
Author(s):  
Todd A. Ponzio ◽  
Raymond L. Fields ◽  
Omar M. Rashid ◽  
Yasmmyn D. Salinas ◽  
Daniel Lubelski ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cell Type ◽  
Specific Expression ◽  
Promoter Deletion ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Vasopressin Gene

Targeted delivery of Chil3/Chil4 siRNA to alveolar macrophages using ternary complexes composed of HMG and oligoarginine micelles

Nanoscale ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 933-943 ◽  
Author(s):  
Moonhwan Choi ◽  
Haeyoon Jeong ◽  
Sol Kim ◽  
Minkyung Kim ◽  
Minhyung Lee ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Alveolar Macrophages ◽  
Targeted Delivery ◽  
Ternary Complexes ◽  
Specific Gene ◽  
Gene Delivery System ◽  
Cell Type ◽  
A Cell ◽  
Cell Type Specific

Cell-type-specific genes involved in disease can be effective therapeutic targets; therefore, the development of a cell-type-specific gene delivery system is essential.

scholarly journals Monitoring Cell-Type–Specific Gene Expression Using Ribosome Profiling In Vivo During Cardiac Hemodynamic Stress

2019 ◽  
Vol 125 (4) ◽  
pp. 431-448 ◽  
Author(s):  
Shirin Doroudgar ◽  
Christoph Hofmann ◽  
Etienne Boileau ◽  
Brandon Malone ◽  
Eva Riechert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ribosome Profiling ◽  
Specific Gene ◽  
Cell Type ◽  
Specific Gene Expression ◽  
Hemodynamic Stress ◽  
Cell Type Specific

Retroviral Vectors Containing Chimeric Promoter/Enhancer Elements Exhibit Cell-Type-Specific Gene Expression

1994 ◽  
Vol 5 (6) ◽  
pp. 667-677 ◽  
Author(s):  
Larry A. Couture ◽  
Craig A. Mullen ◽  
Richard A. Morgan
Keyword(s):  
Gene Expression ◽  
Retroviral Vectors ◽  
Specific Gene ◽  
Cell Type ◽  
Chimeric Promoter ◽  
Specific Gene Expression ◽  
Cell Type Specific

Cell Type-Specific Gene Expression and Editing Responses to Chronic Fluoxetine Treatment in the In Vivo Mouse Brain and Their Relevance for Stress-Induced Anhedonia

2012 ◽  
Vol 37 (11) ◽  
pp. 2480-2495 ◽  
Author(s):  
Baoman Li ◽  
Lu Dong ◽  
Bing Wang ◽  
Liping Cai ◽  
Ning Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mouse Brain ◽  
Specific Gene ◽  
Cell Type ◽  
Specific Gene Expression ◽  
Fluoxetine Treatment ◽  
Chronic Fluoxetine ◽  
Cell Type Specific

scholarly journals Cell type specific gene expression profiling reveals a role for the complement component C3A in neutrophil migration to tissue damage

2019 ◽  
Author(s):  
Ruth A. Houseright ◽  
Emily E. Rosowski ◽  
Pui Ying Lam ◽  
Sebastien JM Tauzin ◽  
Oscar Mulvaney ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacterial Infections ◽  
Large Scale ◽  
Affinity Purification ◽  
Neutrophil Migration ◽  
Acute Injury ◽  
Specific Gene ◽  
Cell Type ◽  
Cell Type Specific

AbstractFollowing acute injury, leukocytes rapidly infiltrate into tissues. For efficient recruitment, leukocytes must sense and respond to signals from both from the damaged tissue and from one another. However, the cell type specific transcriptional changes that influence leukocyte recruitment and wound healing have not been well characterized. In this study, we performed a large-scale translating ribosome affinity purification (TRAP) and RNA sequencing screen in larval zebrafish to identify genes differentially expressed by neutrophils, macrophages, and epithelial cells in the context of wounding. We identified the complement pathway and c3a.1, homologous to the C3A component of human complement, as significantly increased in neutrophils in response to a wound. We report that c3a.1−/− zebrafish larvae have impaired neutrophil responses to both tail wounds and localized bacterial infections, as well as increased susceptibility to infection due to a neutrophil-intrinsic function of C3A. We further show that C3A enhances migration of human primary neutrophils to IL-8 and that c3a.1−/− larvae have impaired neutrophil migration in vivo, and a decrease in neutrophil directed migration speed early after wounding. Together, our findings suggest a role for C3A in mediating efficient neutrophil migration to damaged tissues and support the power of TRAP to identify cell-specific changes in gene expression associated with wound-associated inflammation.

Surface-Engineered Viral Vectors for Selective and Cell Type-Specific Gene Delivery

2015 ◽  
Vol 33 (12) ◽  
pp. 777-790 ◽  
Author(s):  
Christian J. Buchholz ◽  
Thorsten Friedel ◽  
Hildegard Büning
Keyword(s):  
Gene Delivery ◽  
Viral Vectors ◽  
Specific Gene ◽  
Cell Type ◽  
Cell Type Specific
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