Selection of a high-biomass, chromium-rich yeast strain and optimization of cultivation conditions

2001 ◽  
Vol 27 (4) ◽  
pp. 195-198 ◽  
Author(s):  
J Liu ◽  
B Zhang ◽  
X He ◽  
P Zhang ◽  
Z Chai
2019 ◽  
pp. 62-70
Author(s):  
Lyudmila Kabaivanova ◽  
Adriana Goushterova ◽  
Mariya Brazkova ◽  
Petar Grozdanov ◽  
Elena Chorukova ◽  
...  

This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use. Key words: Kluyveromyces sp., inulinase activity, parameters optimization


2019 ◽  
pp. 54-61
Author(s):  
Lyudmila Kabaivanova ◽  
Adriana Goushterova ◽  
Mariya Brazkova ◽  
Petar Grozdanov ◽  
Elena Chorukova ◽  
...  

This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use. Key words: Kluyveromyces sp., inulinase activity, parameters optimization


2021 ◽  
Author(s):  
Tinsae Bahru Yifru ◽  
Berhane Kidane ◽  
Amsalu Tolessa

Abstract Background: In Ethiopia, about 92.3% of all the biomass energy is consumed by domestic households and the demand is growing from 10-14%. However, there are little/no practical experiences or documented indigenous knowledge on how traditional people identify and select high biomass producing plant species with short rotation periods at Boset District. Therefore, the present study was aimed at: (1) selecting and documenting high biomass energy producing plant species at Boset District; (2) identifying major predictor variables that influence the prioritization and selection of species; and (3) develop a Generalized Linear Model (GLM) to predict the selection of species. Methods: A total of 96 informants comprising 59 men and 37 women between the ages of 18 and 81 were sampled. Data were collected using structured interviews, guided field walk, discussions and field observations. Results: Collected data indicated that 88.5% of the informants involved in firewood collection, while 90% practiced charcoal making. A total of 1533.60 Birr per household on average was earned annually from this activity. A total of 25 firewood and/or charcoal plant species were identified and documented at Boset District. Of these, Acacia senegal, Acacia tortilis and Acacia robusta were the three best prioritized and selected indigenous high biomass producing species. Prosopis juliflora, Parthenium hysterophorus, Azadirachta indica, Calotropis procera, Cryptostegia grandiflora, Lantana camara and Senna occidentalis further grouped under introduced fuelwood species. Prediction of GLM assured sampled Kebeles and source of income generated from fuelwood species positively and significantly (p<0.001) related to selection of species. Higher efficiency to provide energy and heat; little or no smoke or soot; easier to cut and split the wood and easier availability were some of the main selection criteria. Conclusions: This study provides valuable information in selection and documenting of high biomass producing plant species for proper management and sustainable use at Boset District. The three most selected species (A. senegal, A. tortilis and A. robusta) should be further evaluated at laboratory to determine their energy values.


Author(s):  
Igor Loskutov ◽  
Lubov Novikova ◽  
Olga Kovaleva ◽  
Nadezhda Ivanova ◽  
Elena Blinova ◽  
...  

Under conditions of climate change, the assessment of the stability of genotypes is of particular importance. To conduct directed selection of genotypes with a narrow or broad reaction rate, it is necessary to assess their stability already in the early stages of breeding. The aim of the study was to study the stability of breeding significant traits of oat and barley samples in contrasting ecological and geographical conditions. 25 oat samples and 25 barley samples were studied over 3 years under contrasting conditions in St. Petersburg and the Tambov region. Varieties are characterized by average values of economically valuable traits and genotype regression coefficients on the influence of the bi environment according to Eberhart and Russell. The most sensitive to a change in the ecological and geographical situation were the durations of the germination-heading, germination-harvest periods and grain yield. These characters varied to a greater extent depending on the cultivation conditions than on the genotype. According to regression coefficients for environmental conditions, significant differences in genotypes were only in yield. Contrasting groups of varieties were distinguished by regression coefficients on environmental conditions, genotypes with high productivity. The durations of germination-heading, germination-harvest, the plant height reacted to the change in the environment the same in different varieties. The duration of the growing season was determined by the sum of effective temperatures above 15C. The reduction of the growing season in both crops was 3 days with an increase in the sum of effective temperatures above 15C by 100C.


AoB Plants ◽  
2014 ◽  
Vol 6 ◽  
Author(s):  
Devesh Singh ◽  
Anne K. Buhmann ◽  
Tim J. Flowers ◽  
Charlotte E. Seal ◽  
Jutta Papenbrock

2010 ◽  
Vol 150 ◽  
pp. 392-392
Author(s):  
Larissa Papin ◽  
Clóvis Parazzi ◽  
Jorge Lopes

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