scholarly journals PARAMETERS OPTIMIZATION FOR INCREASED INTRACELLULAR INULINASE ACTIVITY OF A YEAST STRAIN

2019 ◽  
pp. 54-61
Author(s):  
Lyudmila Kabaivanova ◽  
Adriana Goushterova ◽  
Mariya Brazkova ◽  
Petar Grozdanov ◽  
Elena Chorukova ◽  
...  
Keyword(s):  
Yeast Strain ◽  
Sole Source ◽  
Parameters Optimization ◽  
Activity Assay ◽  
Cultivation Conditions ◽  
Inulinase Activity ◽  
Cultivation Process ◽  
Favorable Conditions ◽  
Extracellular Activity ◽  
Selection Of

This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use. Key words: Kluyveromyces sp., inulinase activity, parameters optimization

scholarly journals PARAMETERS OPTIMIZATION FOR INCREASED INTRACELLULAR INULINASE ACTIVITY OF A YEAST STRAIN

2019 ◽  
pp. 62-70
Author(s):  
Lyudmila Kabaivanova ◽  
Adriana Goushterova ◽  
Mariya Brazkova ◽  
Petar Grozdanov ◽  
Elena Chorukova ◽  
...  
Keyword(s):  
Yeast Strain ◽  
Sole Source ◽  
Parameters Optimization ◽  
Activity Assay ◽  
Cultivation Conditions ◽  
Inulinase Activity ◽  
Cultivation Process ◽  
Favorable Conditions ◽  
Extracellular Activity ◽  
Selection Of

This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use. Key words: Kluyveromyces sp., inulinase activity, parameters optimization

scholarly journals Selection gains for the palm oil production from progenies of American oil palm with oil palm

2021 ◽  
Vol 56 ◽  
Author(s):  
Rui Alberto Gomes Junior ◽  
Alessandra Ferraiolo de Freitas ◽  
Raimundo Nonato Vieira da Cunha ◽  
Antônio José de Abreu Pina ◽  
Higo Otávio Brochado Campos ◽  
...  
Keyword(s):  
Palm Oil ◽  
Oil Palm ◽  
Genetic Parameters ◽  
Recurrent Selection ◽  
Oil Production ◽  
Reciprocal Recurrent Selection ◽  
Fresh Fruit Bunches ◽  
Favorable Conditions ◽  
Selection Of

Abstract The objective of this work was to estimate the genetic parameters, correlations, and selection gains for the oil production of interspecific hybrids progenies between American oil palm, of ‘Manicoré’ origin, and oil palm, of ‘La Mé’ origin. Thirty-nine progenies were evaluated from the sixth to the ninth year after planting, for the productivity of fresh fruit bunches (PROD_FFB), oil content in the bunch (OCB), and palm oil productivity (PROD_OP). The genetic parameters and gains from direct (GDS) and indirect (GIS) selection were estimated for PROD_OP. High values of heritability for the CVg/CVe ratio indicated favorable conditions for the selection. With the selection of 20% of the progenies (selection in both sexes), the following estimates were obtained: 11.15% GDS for PROD_OP, 9.1% GIS for OCB, and 8.1% GIS for PROD_FFB. The PROD_OP of the progenies was of 6,175, 6,057, and 5,995 kg ha-1 per year with GDS and GIS for OCB and PROD_FFB, respectively. The restricted selection of the LM2T male genitor offspring resulted in 5.1% estimated GSD and in a mean of 5,800 kg ha-1 per year for PROD_OP. Selection gains for PROD_OP can be achieved immediately through the selection restricted to oil palm male genitors, and, in the medium and long term, through the interspecific reciprocal recurrent selection between American oil palm and oil palm populations.

scholarly journals ECOLOGICAL-GEOGRAPHIC APPROACHES TO THE STUDY OF GENETIC DIVERSITY OF BARLEY AND OAT FROM THE VIR COLLECTION

2020 ◽  
Author(s):  
Igor Loskutov ◽  
Lubov Novikova ◽  
Olga Kovaleva ◽  
Nadezhda Ivanova ◽  
Elena Blinova ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Growing Season ◽  
Regression Coefficients ◽  
Cultivation Conditions ◽  
High Productivity ◽  
Sum Of Effective Temperatures ◽  
Economically Valuable Traits ◽  
Effective Temperatures ◽  
The Stability ◽  
Selection Of

Under conditions of climate change, the assessment of the stability of genotypes is of particular importance. To conduct directed selection of genotypes with a narrow or broad reaction rate, it is necessary to assess their stability already in the early stages of breeding. The aim of the study was to study the stability of breeding significant traits of oat and barley samples in contrasting ecological and geographical conditions. 25 oat samples and 25 barley samples were studied over 3 years under contrasting conditions in St. Petersburg and the Tambov region. Varieties are characterized by average values of economically valuable traits and genotype regression coefficients on the influence of the bi environment according to Eberhart and Russell. The most sensitive to a change in the ecological and geographical situation were the durations of the germination-heading, germination-harvest periods and grain yield. These characters varied to a greater extent depending on the cultivation conditions than on the genotype. According to regression coefficients for environmental conditions, significant differences in genotypes were only in yield. Contrasting groups of varieties were distinguished by regression coefficients on environmental conditions, genotypes with high productivity. The durations of germination-heading, germination-harvest, the plant height reacted to the change in the environment the same in different varieties. The duration of the growing season was determined by the sum of effective temperatures above 15C. The reduction of the growing season in both crops was 3 days with an increase in the sum of effective temperatures above 15C by 100C.

scholarly journals Salicornia as a crop plant in temperate regions: selection of genetically characterized ecotypes and optimization of their cultivation conditions

AoB Plants ◽  
2014 ◽  
Vol 6 ◽  
Author(s):  
Devesh Singh ◽  
Anne K. Buhmann ◽  
Tim J. Flowers ◽  
Charlotte E. Seal ◽  
Jutta Papenbrock
Keyword(s):  
Crop Plant ◽  
Cultivation Conditions ◽  
Selection Of

Selection of Yeast Strain for Ethanol Production from Sugarcane

2010 ◽  
Vol 150 ◽  
pp. 392-392
Author(s):  
Larissa Papin ◽  
Clóvis Parazzi ◽  
Jorge Lopes
Keyword(s):  
Ethanol Production ◽  
Yeast Strain ◽  
Selection Of

scholarly journals Criteria for damping parameters optimization in seismic isolated structures

2020 ◽  
Vol 17 (5) ◽  
pp. 94-100
Author(s):  
N. V. Ostrovskaya ◽  
◽  
D. E. Bondarev ◽  
Keyword(s):  
Optimization Criterion ◽  
Parameters Optimization ◽  
Optimal Damping ◽  
Insulation Systems ◽  
Seismic Insulation ◽  
Isolated Structures ◽  
Selection Of

All kinds of seismic insulation systems are widely used to protect various structures and buildings, including unique ones, against earthquakes. The efficiency of such systems depends significantly on the competent selection of damping parameters. The article considers a general approach to selecting optimal damping parameters, both by the criterion of absolute accelerations and by the kinematic criterion. The optimization criterion for unique structures is also proposed.

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