Measurement of immunoglobulins in seminal fluid with modified nephelometry—an alternative diagnostic tool for chronic prostatitis

Semen analysis remains the most important diagnostic tool for the study of male infertility to date. For this reason, and because of the ease of carrying out this analysis, examination of seminal fluid should be among the first diagnostic steps in cases of suspected infertility, prior to subjecting the man’s partner to long and complex diagnostic tests. The efficacy of an examination of seminal fluid depends on the experience and ability of the seminologist, who must first undertake a subjective analysis of fundamental parameters such as motility and morphology. Moreover, laboratories specialized in such analyses may apply different criteria to the evaluation of sperm parameters, making it extremely difficult to compare tests carried out in different laboratories (1). In an attempt to resolve these problems of inconsistency, and in order to standardize laboratory techniques, a committee of experts from the WHO established guidelines for semen analysis in 1980 (an updated version was published in 1999) (2). In recent years, numerous other methods of semen analysis capable of providing in-depth diagnostic information on the fertilising capacity of spermatozoa have become available. The computer-aided sperm analysis (CASA) system is a technique for sperm analysis designed to provide objective data on sperm motility (3). Because of persisting difficulties in software set-up (4), it should not be used for routine analysis, but rather as a research tool. At the same time, significant advances have been made in the study of sperm morphology through the use of scanning and transmission electron microscopes (5). Finally, within the past decade several tests capable of evaluating the integrity of sperm components, such as the membrane, acrosome, DNA, and nuclear protein, have been developed and put into use. These more complex and costly analytical tools should be considered of secondary or tertiary importance, and are to be carried out in specific cases only after standard semen analysis. Standard semen analysis remains the first and fundamental diagnostic tool.

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Phenotypic differences between coagulase-negative staphylococci isolated from seminal fluid of healthy men and men suffering from chronic prostatitis syndrome

International Journal of Andrology ◽

10.1111/j.1365-2605.2009.00969.x ◽

2009 ◽

Vol 33 (3) ◽

pp. 563-567 ◽

Cited By ~ 2

Author(s):

Iuri B. Ivanov ◽

Viktor A. Gritsenko ◽

Michael D. Kuzmin

Keyword(s):

Chronic Prostatitis ◽

Seminal Fluid ◽

Coagulase Negative Staphylococci ◽

Healthy Men ◽

Phenotypic Differences ◽

Prostatitis Syndrome

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Phenotypic differences between coryneform bacteria isolated from seminal fluid of healthy men and men with chronic prostatitis syndrome

Asian Journal of Andrology ◽

10.1038/aja.2009.24 ◽

2009 ◽

Vol 11 (4) ◽

pp. 517-520 ◽

Cited By ~ 2

Author(s):

Iuri B Ivanov ◽

Viktor A Gritsenko ◽

Michael D Kuzmin

Keyword(s):

Chronic Prostatitis ◽

Seminal Fluid ◽

Healthy Men ◽

Phenotypic Differences ◽

Coryneform Bacteria ◽

Prostatitis Syndrome

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The role of the level of biochemical markers of inflammation and protective systems of the organism in blood serum and in seminal fluid in patients with chronic prostatitis

Pedagogy and Psychology of Sport ◽

10.12775/pps.2015.02.02.001 ◽

2016 ◽

Vol 2 (2) ◽

pp. 11

Author(s):

V. V. Shuhtin ◽

V. N. Sknar ◽

I. N. Shuhtina ◽

W. Zukow

Keyword(s):

Blood Serum ◽

Biochemical Markers ◽

Chronic Prostatitis ◽

Seminal Fluid ◽

Markers Of Inflammation ◽

Protective Systems

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Transrectal ultrasound in abacterial chronic prostatitis: Identification of a possible reference pattern

Urologia Journal ◽

10.1177/039156039205901s10 ◽

1992 ◽

Vol 59 (1_suppl) ◽

pp. 31-35

Author(s):

G. Conti ◽

E. Gianneo ◽

L. Fumagalli ◽

E. Sala ◽

R. Antinozzi ◽

...

Keyword(s):

Chronic Prostatitis ◽

Seminal Fluid ◽

Ureaplasma Urealyticum ◽

Transrectal Ultrasound ◽

Mycoplasma Hominis ◽

Point Of View ◽

Reference Pattern ◽

Sexually Transmitted ◽

Prostatic Disease ◽

Diagnostic Difficulties

Chronic prostatitis caused by sexually transmitted pathogens (Chlamydia Trachomatis, Ureaplasma Urealyticum, Mycoplasma Hominis) is a quite frequent disease in the mean age of life (20–50 years). Diagnostic difficulties are encountered because of poor symptomatology and inadequacy of common laboratory microbiologic essay. So this disease requires a rather elaborate diagnostic procedure like specific determination of microrganisms in seminal fluid, prostatic and urethral secretion. From the echographic point of view no patognomonic patterns can be referred to at present. The authors identified in a group of 48 patients with proven infection by sexually transmitted pathogens, some echographic findings perhaps indicative of this prostatic disease (more evidence of cranial periurethral prostatic hypoechogenicity in all infected patients, and quite complete resolution in a high percentage of patients after microrganism eradication). If confirmed by a higher number of observations, this data may become a useful diagnostic instrument in the approach to sexually transmitted prostatic disease in order to support and confirm the diagnosis and therapy.

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EFFICIENCY OF SERENOA REPENS EXTRACT IN TREATMENT OF EXCTRETRIC-TOXIC INFERTILITY, ASSOCIATED WITH CHRONIC PROSTATITIS

Men’s Health, Gender and Psychosomatic Medicine ◽

10.37321/ujmh.2018.01-04 ◽

2018 ◽

pp. 91-96

Author(s):

Юрий Заседа

Keyword(s):

Antibiotic Therapy ◽

Chronic Prostatitis ◽

Seminal Fluid ◽

Reproductive Technologies ◽

Routine Practice ◽

Interleukin 18 ◽

Serenoa Repens ◽

Reproductive Process ◽

Biochemical Research ◽

Therapeutic Model

Relevance. The development of reproductive technologies and techniques, the establishment of biological and immunological aspects of the reproductive process and the expansion of the use of biochemical research in routine practice, has led to the increasing attention to androurological disorders.The aim of the study is a comparative analysis of the effectiveness of the therapeutic model using a combination of Serenoa repens extract (in the composition of the drug “Prostamol Uno”), selective antibiotic therapy and physiotherapy in the treatment of excretory-toxic infertility associated with chronic prostatitis.Materials and methods. The study was conducted in a prospective study on parallel groups. The contingent of the study comprised 100 patients of the Clinic “Men’s Health” (Kiev), suff ering from chronic prostatitis and undergoing outpatient treatment for fertility disorders.Results of the study. A comparison of the eff ectiveness of the therapeutic model using a combination of Serenoa repens extract (as part of the Prostamol Uno preparation), selective antibiotic therapy and physiotherapy, and a similar model without prescribing Prostamol Uno for the treatment of excretory-toxic infertility associated with chronic prostatitis.Comparison criteria were the data of the expanded spermogram, the clinical questionnaire I-PSS and the level of interleukin-18 in the seminal plasma (established by the method of enzyme immunoassay).Conclusions. The data confi rming the greater eff ectiveness of the model with respect to the spermiogram indices, namely: the volume and time of liquefaction of the seminal fluid; the number of spermatozoa in 1 ml of seminal fluid; presence of spermatozoa (group A + B); the number of leukocytes in the fi eld of vision; amount of mucus in the semen.Also, when analyzing the clinical eff ects of the model in question, there are more pronounced changes in the symptoms of prostatitis, namely: rapid urination; weakening of the urine stream; reducing the quality of life; of the overall I-PSS score. The reliable effi cacy of the study model of treatment in normalizing the level of interleukin-18 in plasma of seminal fluid was also established.

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