Development of a double-antibody sandwich ELISA for rapid detection of Bacillus Cereus in food

ABSTRACT Bacillus cereus has been reported as a foodborne pathogen worldwide. Although food processing technologies to inactivate the pathogen have been developed for decades, foodborne outbreaks related to B. cereus have occurred. In the present review, foodborne outbreaks, germination, inactivation, and detection of B. cereus are discussed, along with inactivation mechanisms. B. cereus outbreaks from 2003 to 2016 are reported based on food commodity, number of cases, and consequent illnesses. Germination before sporicidal treatments is highlighted as an effective way to inactivate B. cereus, because the resistance of the pathogen increases significantly following sporulation. Several germinants used for B. cereus are listed, and their efficacies are compared. Finally, recently used interventions with sporicidal mechanisms are identified, and rapid detection methods that have been developed are discussed. Combining two or more interventions, known as the hurdle technology concept, is suggested to maximize the sporicidal effect. Further study is needed to ensure food safety and to understand germination mechanisms and sporicidal resistance of B. cereus. HIGHLIGHTS

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LIGHT SCATTERING SENSOR FOR RAPID DETECTION OF BACILLUS CEREUS IN CHICKEN MEATS

Mansoura Veterinary Medical Journal ◽

10.21608//mvmj.2018.19.1212 ◽

2018 ◽

Vol 19 (1) ◽

pp. 57-68

Author(s):

Maha Abdalhaseib

Keyword(s):

Light Scattering ◽

Bacillus Cereus ◽

Rapid Detection

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Development of double-antibody sandwich ELISA for rapidly quantitative detection of antigen concentration in inactivated SCRV vaccine

Aquaculture ◽

10.1016/j.aquaculture.2019.734671 ◽

2020 ◽

Vol 520 ◽

pp. 734671 ◽

Cited By ~ 2

Author(s):

Yinjie Niu ◽

Peng Zhang ◽

Luyao Wang ◽

Ningqiu Li ◽

Qiang Lin ◽

...

Keyword(s):

Sandwich Elisa ◽

Quantitative Detection ◽

Antigen Concentration ◽

Double Antibody

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Rapid Detection of Salmonella spp. in Foods by Combination of a New Selective Enrichment and a Sandwich ELISA Using Two Monoclonal Antibodies against Dulcitol 1-Phosphate Dehydrogenase

Journal of Food Protection ◽

10.4315/0362-028x-59.11.1158 ◽

1996 ◽

Vol 59 (11) ◽

pp. 1158-1163 ◽

Cited By ~ 11

Author(s):

HUAIZE TIAN ◽

TAKAHISA MIYAMOTO ◽

TAKASHI OKABE ◽

YOICHIRO KURAMITSU ◽

KEN-ICHI HONJOH ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Rapid Detection ◽

Enrichment Culture ◽

Sandwich Elisa ◽

False Negative ◽

Detection Sensitivity ◽

Elisa Method ◽

Salmonella Spp ◽

Selective Enrichment ◽

Raw Foods

A rapid-detection method was developed for food-borne dulcitol-positive Salmonella spp. in foods that involves a new preenrichment and selective enrichment system and a sandwich ELISA using two monoclonal antibodies against dulcitol 1-phosphate dehydrogenase. Preenrichment and selective enrichment were in Enterobacteriaceae enrichment mannitol (EEM) broth at 42°C for 6 h and in a new dulcitol-magnesium chloride-pyridinesulfonic acid brilliant green-novobiocin (DMPBN) medium at 42°C for 27 h, respectively. The cells were collected from the selective enrichment culture and suspended in 0.1 ml of 1 N NaOH for 2 min. The solution was neutralized with 0.1 ml of 2 M Tris-HCl buffer (pH 7.5) and the mixture was used as a sample for ELISA. The detection sensitivity of the ELISA was 105 CFU of Salmonella spp. per ml of culture. Competing non-Salmonella organisms in raw food did not interfere with the detection of Salmonella cells even when present at 107: 1 (non-Salmonella: Salmonella ratio) in food. Nonmotile Salmonella gallinarum was detected by the ELISA. The minimum detectable number of initial inoculum of Salmonella typhimurium was 0.69 CFU/25 g of raw chicken after the preenrichment in EEM broth and the selective enrichment in DMPBN medium. The present ELISA method required a total analysis time of 36 h including the preenrichment and selective enrichment periods. The ELISA method was compared with a conventional cultural method for the detection of Salmonella cells in 130 samples of raw foods. Of the samples tested, 16 were Salmonella-positive and 114 samples were negative by both methods. False-positive and false-negative results were not encountered.

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