scholarly journals Zinc Resistance Mechanisms of P1B-type ATPases in Sinorhizobium meliloti CCNWSX0020

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mingmei Lu ◽  
Zhefei Li ◽  
Jianqiang Liang ◽  
Yibing Wei ◽  
Christopher Rensing ◽  
...  
Keyword(s):  
Sinorhizobium Meliloti ◽  
Resistance Mechanisms ◽  
Zinc Resistance

scholarly journals Reprograming of sRNA target specificity by the leader peptide peTrpL in response to antibiotic exposure

2021 ◽  
Vol 49 (5) ◽  
pp. 2894-2915
Author(s):  
Hendrik Melior ◽  
Siqi Li ◽  
Maximilian Stötzel ◽  
Sandra Maaß ◽  
Rubina Schütz ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Posttranscriptional Regulation ◽  
Ribosomal Proteins ◽  
Sinorhizobium Meliloti ◽  
Resistance Mechanisms ◽  
Leader Peptide ◽  
In Vitro Reconstitution ◽  
Biosynthesis Gene ◽  
Regulatory Rnas

Abstract Trans-acting regulatory RNAs have the capacity to base pair with more mRNAs than generally detected under defined conditions, raising the possibility that sRNA target specificities vary depending on the specific metabolic or environmental conditions. In Sinorhizobium meliloti, the sRNA rnTrpL is derived from a tryptophan (Trp) transcription attenuator located upstream of the Trp biosynthesis gene trpE(G). The sRNA rnTrpL contains a small ORF, trpL, encoding the 14-aa leader peptide peTrpL. If Trp is available, efficient trpL translation causes transcription termination and liberation of rnTrpL, which subsequently acts to downregulate the trpDC operon, while peTrpL is known to have a Trp-independent role in posttranscriptional regulation of antibiotic resistance mechanisms. Here, we show that tetracycline (Tc) causes rnTrpL accumulation independently of Trp availability. In the presence of Tc, rnTrpL and peTrpL act collectively to destabilize rplUrpmA mRNA encoding ribosomal proteins L21 and L27. The three molecules, rnTrpL, peTrpL, and rplUrpmA mRNA, form an antibiotic-dependent ribonucleoprotein complex (ARNP). In vitro reconstitution of this ARNP in the presence of competing trpD and rplU transcripts revealed that peTrpL and Tc cause a shift of rnTrpL specificity towards rplU, suggesting that sRNA target prioritization may be readjusted in response to changing environmental conditions.

Resistance mechanisms ofPrunusrootstocks to root-knot nematode,Meloidogyne incognita

Fruits ◽  
2009 ◽  
Vol 64 (5) ◽  
pp. 295-303 ◽  
Author(s):  
Hang Ye ◽  
Wen-jun Wang ◽  
Guo-jie Liu ◽  
Li-xin Zhu ◽  
Ke-gong Jia
Keyword(s):  
Meloidogyne Incognita ◽  
Resistance Mechanisms ◽  
Root Knot Nematode

Emerging Antimicrobial Resistance Among Methicillin Resistant Staphylococcus Aureus (MRSA) in a Tertiary Care Centre in North India

JMS SKIMS ◽  
2020 ◽  
Vol 23 (1) ◽  
pp. 48-49
Author(s):  
Javaid Ahmad Bhat ◽  
Shariq Rashid Masoodi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Tertiary Care ◽  
Resistance Mechanisms ◽  
North India ◽  
Global Public Health ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Effective Prevention ◽  
Mupirocin Resistance

Apropos to the article by Dr Bali, titled “Mupirocin resistance in clinical isolates of methicillin-sensitive and resistant Staphylococcus aureus in a tertiary care centre of North India” (1), the authors have raised important issue of emerging antimicrobial resistance (AMR). Antimicrobial resistance is an increasingly serious threat to global public health that requires action across all government sectors and society. As per WHO, AMR lurks the effective prevention and management of an ever-increasing spectrum of infections caused by bacteria, parasites, fungi and viruses. Novel resistance mechanisms are emerging and spreading globally, threatening the man’s ability to treat common infectious diseases.

Rhizobium törzsek túléloképessége különbözo vivoanyagokban

2003 ◽  
Vol 52 (3-4) ◽  
pp. 395-408 ◽  
Author(s):  
László Ködöböcz ◽  
Borbála Biró ◽  
Ilona Dusha ◽  
Zoltánné Izsáki ◽  
Lajos Sáry ◽  
...  
Keyword(s):  
Sinorhizobium Meliloti

A lucerna magoltására alkalmas két Sinorhizobium meliloti törzs túléloképességét ellenoriztük laboratóriumi körülmények között. Az oltás szempontjait figyelembe véve a baktériumtörzsek túléloképességét a magfelszínen, folyékony táptalajban, talajban és mezogazdasági hulladék felhasználásával készített komposztban ellenoriztük 1-6 hetes idotartamban. A kiindulási sejtszámhoz képest a törzsek túléloképességét (pusztulását és esetleges szaporodását) az általunk módosított, szelektív táplemezes kitenyésztéssel követtük nyomon antibiotikum marker segítségével. Megállapítást nyert, hogy a különféle lucernamagvak felszínén a Rhizobium törzsek túléloképességét a fajtatulajdonságok erosen befolyásolják. A fajták és az oltóanyagok közötti kompatibilitás-vizsgálatoknak tehát a mikrobiális oltóanyagok alkalmazásánál nagy jelentosége van.  Talajokban a Rhizobiumok perzisztenciáját a talajok kötöttsége, alacsony pH-ja és a tápanyag-szegény körülmények is gátolhatják. Az alkalmazott törzsek vivoanyagának a nagy szerves- és tápanyagtartalmú komposztok a legmegfelelobbek. Az anaerob módon eloállított komposzt a vizsgált törzsek túléloképességéhez kedvezobb körülményeket biztosít. Ennek oka feltételezésünk szerint a biotikus tényezok kedvezobb alakulása, azaz az anaerob körülményekhez adaptálódott konkurens mikrobák kisebb fokú versenyképessége lehet. Feltételezéseinket a sterilizált komposztban kimutatott nagyobb mértéku túléloképesség támasztja alá.  A megfelelo komposztok ilyen irányú felhasználásával kiváltható a tozegek alkalmazása, az oltóanyag-eloállítási technológia gazdaságosabbá teheto.

Exemplar Abstract for Sinorhizobium meliloti (Dangeard 1926) De Lajudie et al. 1994.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Sinorhizobium Meliloti

Exemplar Abstract for Sinorhizobium meliloti (Dangeard 1926) De Lajudie et al. 1994.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Sinorhizobium Meliloti

Nomenclature Abstract for Sinorhizobium meliloti (Dangeard 1926) De Lajudie et al. 1994.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Sinorhizobium Meliloti

Evaluation of Potent Isoquinoline-Based Thiosemicarbazone Antiproliferatives Against Solid Tumor Models

2019 ◽  
Author(s):  
Daniel Sun ◽  
Soumya Poddar ◽  
Roy D. Pan ◽  
Juno Van Valkenburgh ◽  
Ethan Rosser ◽  
...  
Keyword(s):  
Solid Tumor ◽  
Small Cell Lung Carcinoma ◽  
Single Agent ◽  
Resistance Mechanisms ◽  
Tumor Models ◽  
Cell Lung Carcinoma ◽  
Adaptive Resistance ◽  
Cancer Models ◽  
Nanomolar Range

The lead compound, an ⍺-N-heterocyclic carboxaldehyde thiosemicarbazone <b>HCT-13</b>, was highly potent against a panel of pancreatic, small cell lung carcinoma, and prostate cancer models, with IC<sub>90</sub> values in the low-to-mid nanomolar range.<b> </b>We show that the cytotoxicity of <b>HCT-13</b> is copper-dependent, that it acts as a copper ionophore, induces production of reactive oxygen species (ROS), and promotes mitochondrial dysfunction and S-phase arrest. Lastly, DNA damage response/replication stress response (DDR/RSR) pathways, specifically Ataxia-Telangiectasia Mutated (ATM) and Rad3-related protein kinase (ATR), were identified as actionable adaptive resistance mechanisms following <b>HCT-13 </b>treatment. Taken together, <b>HCT-13 </b>is potent against solid tumor models and warrants <i>in vivo</i> evaluation against aggressive tumor models, either as a single agent or as part of a combination therapy.

5-Benzylidene-4-Oxazolidinones are Synergistic with Antibiotics for the Treatment of Staphylococcus Aureus Biofilms

2019 ◽  
Author(s):  
Bram Frohock ◽  
Jessica M. Gilbertie ◽  
Jennifer C. Daiker ◽  
Lauren V. Schnabel ◽  
Joshua Pierce
Keyword(s):  
Staphylococcus Aureus ◽  
Human Health ◽  
Matrix Model ◽  
Bacterial Load ◽  
Collagen Matrix ◽  
Resistance Mechanisms ◽  
Novel Therapeutics ◽  
Innovative Treatment ◽  
Antibiotic Action ◽  
Biofilm Infection

<div>The failure of frontline antibiotics in the clinic is one of the most serious threats to human health and requires a multitude of novel therapeutics and innovative treatment approaches to curtail the growing crisis. In addition to traditional resistance mechanisms resulting in the lack of efficacy of many antibiotics, most chronic and recurring infections are further made tolerant to antibiotic action by the presence of biofilms. Herein, we report an expanded set of 5-benzylidene-4-oxazolidinones that are able to inhibit the formation of Staphylococcus aureus biofilms, disperse preformed biofilms and in combination with common antibiotics are able to significantly reduce the bacterial load in a robust collagen-matrix model of biofilm infection.</div>

MICROORGANISMS ANTIBIOTIC RESISTANCE MECHANISMS – PRESENT VIEW

2019 ◽  
Vol 21 (10) ◽  
pp. 125-130
Author(s):  
Пушилина А.Д. ◽  
◽  
Коменкова Т.С. ◽  
Зайцева Е.А. ◽  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Mechanisms ◽  
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