scholarly journals A smart sealed nucleic acid biosensor based on endogenous reference gene detection to screen and identify mammals on site

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yuancong Xu ◽  
Wenjin Xiang ◽  
Qin Wang ◽  
Nan Cheng ◽  
Li Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Reference Gene ◽  
Gene Detection ◽  
Endogenous Reference Gene ◽  
Endogenous Reference

A papaya-specific gene, papain, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic papayas

2008 ◽  
Vol 228 (2) ◽  
pp. 301-309 ◽  
Author(s):  
Wentao Xu ◽  
Weibin Bai ◽  
Feng Guo ◽  
Yunbo Luo ◽  
Yanfang Yuan ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Reference Gene ◽  
Pcr Detection ◽  
Specific Gene ◽  
Real Time Quantitative Pcr ◽  
Endogenous Reference Gene ◽  
Endogenous Reference

Applicability of theChymopapainGene Used as Endogenous Reference Gene for Transgenic Huanong No. 1 Papaya Detection

2009 ◽  
Vol 57 (15) ◽  
pp. 6502-6509 ◽  
Author(s):  
Jinchao Guo ◽  
Litao Yang ◽  
Xin Liu ◽  
Haibo Zhang ◽  
Bingjun Qian ◽  
...  
Keyword(s):  
Reference Gene ◽  
Endogenous Reference Gene ◽  
Endogenous Reference

Identification of a chicken ( Gallus gallus ) endogenous reference gene ( Actb ) and its application in meat adulteration

2017 ◽  
Vol 234 ◽  
pp. 472-478 ◽  
Author(s):  
Wenjin Xiang ◽  
Ying Shang ◽  
Qin Wang ◽  
Yuancong Xu ◽  
Pengyu Zhu ◽  
...  
Keyword(s):  
Reference Gene ◽  
Gallus Gallus ◽  
Endogenous Reference Gene ◽  
Endogenous Reference

Validation of Artemisia Genus-Specific Gene, GEL4, for Use as an Endogenous Reference Gene in Qualitative PCR Detection of Putative Transgenic Artemisia annua

2013 ◽  
Vol 96 (3) ◽  
pp. 587-592
Author(s):  
Hua Liu ◽  
Lingxi Jiang ◽  
Furong Tan ◽  
Xinghai Zhao ◽  
Heyan Lin ◽  
...  
Keyword(s):  
Reference Gene ◽  
Artemisia Annua ◽  
Intraspecific Variability ◽  
Specific Gene ◽  
Endogenous Reference Gene ◽  
Medical Plant ◽  
Endogenous Reference ◽  
Pcr Method ◽  
Artemisinin Content ◽  
Qualitative Pcr

Abstract Artemisia annua L. is a popular medical plant used against malaria. Transgenic A. annua plants have been developed to increase the artemisinin content using genetic engineering. To develop a reliable PCR method of detecting transgenic A. annua, one Artemisia specific gene, GEL4, was selected and validated as suitable. Qualitative PCR methods were assayed with 16 different A. annua ecotypes and four Artemisia plants, and identical amplified products were obtained. No amplified products were observed when genomic DNA samples were extracted from 15 different plant species. An LOD of 0.05 ng of A. annua DNA was also determined. Furthermore, the results of a Southern blot method showed that the GEL4 gene was in three copies of the six A. annua ecotypes tested. To determine whether there was intraspecific variability in the GEL4 region between different A. annua ecotypes, the GEL4 region corresponding to the 10 A. annua ecotypes was cloned and sequenced. Alignment of the obtained sequences presented a highly conserved amplification product region in 10 A. annua ecotypes. Therefore, it is concluded that the GEL4 gene can be used as an endogenous reference gene of A. annua, and that the qualitative PCR system was reliable for the detection of transgenic A. annua.

A peach (Prunus persica)-specific gene, Lhcb2, used as an endogenous reference gene for qualitative and real-time quantitative PCR to detect fruit products

LWT ◽  
2014 ◽  
Vol 55 (1) ◽  
pp. 218-223 ◽  
Author(s):  
Ying Shang ◽  
Pengyu Zhu ◽  
Kunlun Huang ◽  
Weihong Liu ◽  
Wenying Tian ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Reference Gene ◽  
Prunus Persica ◽  
Specific Gene ◽  
Real Time Quantitative Pcr ◽  
Endogenous Reference Gene ◽  
Endogenous Reference

Validation of a cotton-specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons

2005 ◽  
Vol 24 (4) ◽  
pp. 237-245 ◽  
Author(s):  
Litao Yang ◽  
Jianxiu Chen ◽  
Cheng Huang ◽  
Yuhui Liu ◽  
Shirong Jia ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Reference Gene ◽  
Pcr Detection ◽  
Specific Gene ◽  
Real Time Quantitative Pcr ◽  
Endogenous Reference Gene ◽  
Endogenous Reference

scholarly journals Assessment of endogenous reference gene suitability for serum exosomal microRNA expression analysis in liver carcinoma resection studies

2015 ◽  
Vol 12 (3) ◽  
pp. 4683-4691 ◽  
Author(s):  
YI LI ◽  
GUI-MING XIANG ◽  
LIN-LIN LIU ◽  
CHANG LIU ◽  
FEI LIU ◽  
...  
Keyword(s):  
Reference Gene ◽  
Expression Analysis ◽  
Microrna Expression ◽  
Liver Carcinoma ◽  
Endogenous Reference Gene ◽  
Endogenous Reference ◽  
Exosomal Microrna
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