Patterning of DNA nanostructures on silicon surface by electron beam lithography of self-assembled monolayer

2004 ◽  
pp. 786 ◽  
Author(s):  
Guo-Jun Zhang ◽  
Takashi Tanii ◽  
Takashi Funatsu ◽  
Iwao Ohdomari
2007 ◽  
Vol 7 (2) ◽  
pp. 410-417 ◽  
Author(s):  
Guo-Jun Zhang ◽  
Takashi Tanii ◽  
Yuzo Kanari ◽  
Iwao Ohdomari

We report on a flexible method of producing antibody (IgG) nanopatterns by combining electron beam (EB) lithography and a perfluorodecyltriethoxysilane (FDTES) self-assembled monolayer (SAM). Using EB lithography of the FDTES SAM, we easily fabricated IgG patterns with feature sizes on the order of 100 nm. The patterned IgG retained its ability to interact specifically with an anti-IgG. The influence of different concentrations of the IgG and anti-IgG on the resulting fluorescent IgG arrays was investigated. These IgG nanopatterns appeared to be remarkably well controlled and showed almost no detectable nonspecific binding of proteins on a hydrophobic SAM under a suitable incubation condition, characterized by atomic force microscopy, and epi-fluorescence microscopy. The technique enables the realization of high-throughput protein nanoscale arrays with high specificity.


Langmuir ◽  
2003 ◽  
Vol 19 (23) ◽  
pp. 9748-9758 ◽  
Author(s):  
Xuejun Wang ◽  
Wenchuang Hu ◽  
Rajagopal Ramasubramaniam ◽  
Gary H. Bernstein ◽  
Gregory Snider ◽  
...  

2004 ◽  
Vol 43 (7A) ◽  
pp. 4396-4397 ◽  
Author(s):  
Takashi Tanii ◽  
Takumi Hosaka ◽  
Takeo Miyake ◽  
Iwao Ohdomari

1994 ◽  
Vol 64 (3) ◽  
pp. 390-392 ◽  
Author(s):  
C. R. K. Marrian ◽  
F. K. Perkins ◽  
S. L. Brandow ◽  
T. S. Koloski ◽  
E. A. Dobisz ◽  
...  

2003 ◽  
Vol 82 (21) ◽  
pp. 3776-3778 ◽  
Author(s):  
A. Küller ◽  
W. Eck ◽  
V. Stadler ◽  
W. Geyer ◽  
A. Gölzhäuser

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