Chemical alterations to murine brain tissue induced by formalin fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesis

The Analyst ◽  
2011 ◽  
Vol 136 (14) ◽  
pp. 2941 ◽  
Author(s):  
Mark J. Hackett ◽  
James A. McQuillan ◽  
Fatima El-Assaad ◽  
Jade B. Aitken ◽  
Aviva Levina ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Formalin Fixation ◽  
Disease Pathogenesis ◽  
Murine Brain

scholarly journals Effects of Formalin Fixation, Paraffin Embedding, and Time of Storage on DNA Preservation in Brain Tissue: A BrainNet Europe Study

2007 ◽  
Vol 17 (3) ◽  
pp. 297-303 ◽  
Author(s):  
Isidre Ferrer ◽  
Judith Armstrong ◽  
Sabina Capellari ◽  
Piero Parchi ◽  
Thomas Arzberger ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Formalin Fixation ◽  
Dna Preservation ◽  
Paraffin Embedding

scholarly journals Epigenomics and transcriptomics analyses of multiple system atrophy brain tissue supports a role for inflammatory processes in disease pathogenesis

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Conceição Bettencourt ◽  
Ignazio S. Piras ◽  
Sandrine C. Foti ◽  
Joshua Talboom ◽  
Yasuo Miki ◽  
...  
Keyword(s):  
Multiple System Atrophy ◽  
Brain Tissue ◽  
Disease Pathogenesis ◽  
Inflammatory Processes

scholarly journals Profile analysis of hepatic porcine and murine brain tissue slices obtained with a vibratome

2014 ◽  
Author(s):  
Giorgio Mattei ◽  
Irene Cristiani ◽  
Chiara Magliaro ◽  
Arti Ahluwalia
Keyword(s):  
Brain Tissue ◽  
Profile Analysis ◽  
Brain Slices ◽  
Slice Thickness ◽  
Tissue Stiffness ◽  
Quantitative Investigation ◽  
Step Size ◽  
Tissue Slices ◽  
Porcine Liver ◽  
Murine Brain

This study is aimed at characterizing soft tissue slices using a vibratome. In particular, the effect of two sectioning parameters (i.e. step size and sectioning speed) on resultant slice thickness was investigated for fresh porcine liver as well as for paraformaldehyde-fixed (PFA-fixed) and fresh murine brain. A simple framework for embedding, sectioning and imaging the slices was established to derive their thickness, which was evaluated through a purposely developed graphical user interface. Sectioning speed and step size had little effect on the thickness of fresh liver slices. Conversely, the thickness of PFA-fixed murine brain slices was found to be dependent on the step size, but not on the sectioning speed. In view of these results, fresh brain tissue was sliced varying the step size only, which was found to have a significant effect on resultant slice thickness. Although precision-cut slices (i.e. with regular thickness) were obtained for all the tissues, slice accuracy (defined as the match between the nominal step size chosen and the actual slice thickness obtained) was found to increase with tissue stiffness from fresh liver to PFA-fixed brain. This quantitative investigation can be very helpful for establishing the most suitable slicing setup for a given tissue.

scholarly journals Profile analysis of hepatic porcine and murine brain tissue slices obtained with a vibratome

2014 ◽  
Author(s):  
Giorgio Mattei ◽  
Irene Cristiani ◽  
Chiara Magliaro ◽  
Arti Ahluwalia
Keyword(s):  
Brain Tissue ◽  
Profile Analysis ◽  
Brain Slices ◽  
Slice Thickness ◽  
Tissue Stiffness ◽  
Quantitative Investigation ◽  
Step Size ◽  
Tissue Slices ◽  
Porcine Liver ◽  
Murine Brain

This study is aimed at characterizing soft tissue slices using a vibratome. In particular, the effect of two sectioning parameters (i.e. step size and sectioning speed) on resultant slice thickness was investigated for fresh porcine liver as well as for paraformaldehyde-fixed (PFA-fixed) and fresh murine brain. A simple framework for embedding, sectioning and imaging the slices was established to derive their thickness, which was evaluated through a purposely developed graphical user interface. Sectioning speed and step size had little effect on the thickness of fresh liver slices. Conversely, the thickness of PFA-fixed murine brain slices was found to be dependent on the step size, but not on the sectioning speed. In view of these results, fresh brain tissue was sliced varying the step size only, which was found to have a significant effect on resultant slice thickness. Although precision-cut slices (i.e. with regular thickness) were obtained for all the tissues, slice accuracy (defined as the match between the nominal step size chosen and the actual slice thickness obtained) was found to increase with tissue stiffness from fresh liver to PFA-fixed brain. This quantitative investigation can be very helpful for establishing the most suitable slicing setup for a given tissue.

Aluminium-Adjuvanted Vaccines Transiently Increase Aluminium Levels in Murine Brain Tissue

1992 ◽  
Vol 70 (4) ◽  
pp. 278-280 ◽  
Author(s):  
K. Redhead ◽  
G. J. Quinlan ◽  
R. G. Das ◽  
J. M. C. Gutteridge
Keyword(s):  
Brain Tissue ◽  
Murine Brain

scholarly journals Dynamic mechanical properties of murine brain tissue using micro-indentation

2015 ◽  
Vol 48 (12) ◽  
pp. 3213-3218 ◽  
Author(s):  
D.B. MacManus ◽  
B. Pierrat ◽  
J.G. Murphy ◽  
M.D. Gilchrist
Keyword(s):  
Mechanical Properties ◽  
Brain Tissue ◽  
Dynamic Mechanical Properties ◽  
Dynamic Mechanical ◽  
Murine Brain ◽  
Micro Indentation

Transplantation of immature murine brain tissue into the anterior eye-chamber of adult rats

1989 ◽  
Vol 100 (1-3) ◽  
pp. 17-22 ◽  
Author(s):  
Steen Jensen ◽  
Torben Sørensen ◽  
Bente R. Finsen ◽  
Jens Zimmer
Keyword(s):  
Brain Tissue ◽  
Adult Rats ◽  
Murine Brain

scholarly journals Effects of Alzheimer’s disease and formalin fixation on the different mineralised-iron forms in the human brain

2020 ◽  
Author(s):  
Louise van der Weerd ◽  
Anton Lefering ◽  
Andrew Webb ◽  
Ramon Egli ◽  
Lucia Bossoni
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Brain Tissue ◽  
Formalin Fixation ◽  
Magnetic Carriers ◽  
Post Mortem Brain ◽  
Brain Material ◽  
Alzheimer’S Pathology ◽  
The Brain ◽  
Iron Forms

ABSTRACTIron accumulation in the brain is a phenomenon common to many neurodegenerative diseases, perhaps most notably Alzheimer’s disease (AD).We present here magnetic analyses of post-mortem brain tissue of patients who had severe Alzheimer’s disease, and compare the results with those from healthy controls. Isothermal remanent magnetization experiments were performed to assess the extent to which different magnetic carriers are affected by AD pathology and formalin fixation.While Alzheimer’s brain material did not show higher levels of magnetite/maghemite nanoparticles than corresponding controls, the ferrihydrite mineral, known to be found within the core of ferritin proteins and hemosiderin aggregates, almost doubled in concentration in patients with Alzheimer’s pathology, strengthening the conclusions of our previous studies. As part of this study, we also investigated the effects of sample preparation, by performing experiments on frozen tissue as well as tissue which had been fixed in formalin for a period of five months. Our results showed that the two different preparations did not critically affect the concentration of magnetic carriers in brain tissue, as observable by SQUID magnetometry.

IR and Raman imaging of murine brains from control and ApoE/LDLR−/− mice with advanced atherosclerosis

The Analyst ◽  
2016 ◽  
Vol 141 (18) ◽  
pp. 5329-5338 ◽  
Author(s):  
Kamila Kochan ◽  
Karolina Chrabaszcz ◽  
Barbara Szczur ◽  
Edyta Maslak ◽  
Jakub Dybas ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Raman Imaging ◽  
Biochemical Profile ◽  
Murine Brain ◽  
Ir And Raman

IR and Raman imaging combined with chemometric analyses were used to study the biochemical profile of the murine brain tissue from control (C57BL/6J) and ApoE/LDLR−/− mice with advanced atherosclerosis.

scholarly journals Effects of Alzheimer’s disease and formalin fixation on the different mineralised-iron forms in the human brain

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Louise van der Weerd ◽  
Anton Lefering ◽  
Andrew Webb ◽  
Ramon Egli ◽  
Lucia Bossoni
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Brain Tissue ◽  
Formalin Fixation ◽  
Magnetic Carriers ◽  
Post Mortem Brain ◽  
Brain Material ◽  
Alzheimer’S Pathology ◽  
The Brain ◽  
Iron Forms

Abstract Iron accumulation in the brain is a phenomenon common to many neurodegenerative diseases, perhaps most notably Alzheimer’s disease (AD). We present here magnetic analyses of post-mortem brain tissue of patients who had severe Alzheimer’s disease, and compare the results with those from healthy controls. Isothermal remanent magnetization experiments were performed to assess the extent to which different magnetic carriers are affected by AD pathology and formalin fixation. While Alzheimer’s brain material did not show higher levels of magnetite/maghemite nanoparticles than corresponding controls, the ferrihydrite mineral, known to be found within the core of ferritin proteins and hemosiderin aggregates, almost doubled in concentration in patients with Alzheimer’s pathology, strengthening the conclusions of our previous studies. As part of this study, we also investigated the effects of sample preparation, by performing experiments on frozen tissue as well as tissue which had been fixed in formalin for a period of 5 months. Our results showed that the two different preparations did not critically affect the concentration of magnetic carriers in brain tissue, as observable by SQUID magnetometry.

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