Preparation of chlorophyll-a and chlorophyll-b by means of column chromatography with Sephasorb HP Ultrafine

Author(s):  
Keiji Iriyama ◽  
Masahiko Yoshiura ◽  
Masaru Shiraki
1979 ◽  
Vol 52 (8) ◽  
pp. 2383-2385 ◽  
Author(s):  
Masahiko Yoshiura ◽  
Keiji Iriyama ◽  
Masaru Shiraki ◽  
Akira Okada

1973 ◽  
Vol 37 ◽  
Author(s):  
N. Lust

Pigment content of ashes grown up under different circumstances - The pigment content (chlorophyll a, chlorophyll b,  xanthophyll and carotene) has been researched with ashes grown up under  different light circumstances and varying in age and height.     The results prove that the general laws concerning the influence of light  on the pigment content, don’t always work.     The phenomen is very complex. The light quantity is very important in some  cases, but insignificant in others. It seems origin and height of plants have  a strong influence. The results prove also the influence of the environment  is much higher on small plants as on big ones.     The research indicates finally the correlation between the green pigments,  the yellow pigments, and between the green pigments on the one side and the  yellow ones on the other side.


Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 608
Author(s):  
Fairuz Fatini Mohd Yusof ◽  
Jamilah Syafawati Yaacob ◽  
Normaniza Osman ◽  
Mohd Hafiz Ibrahim ◽  
Wan Abd Al Qadr Imad Wan-Mohtar ◽  
...  

The growing demand for high value aromatic herb Polygonum minus-based products have increased in recent years, for its antioxidant, anticancer, antimicrobial, and anti-inflammatory potentials. Although few reports have indicated the chemical profiles and antioxidative effects of Polygonum minus, no study has been conducted to assess the benefits of micro-environmental manipulation (different shading levels) on the growth, leaf gas exchange and secondary metabolites in Polygonum minus. Therefore, two shading levels (50%:T2 and 70%:T3) and one absolute control (0%:T1) were studied under eight weeks and 16 weeks of exposures on Polygonum minus after two weeks. It was found that P. minus under T2 obtained the highest photosynthesis rate (14.892 µmol CO2 m−2 s−1), followed by T3 = T1. The increase in photosynthesis rate was contributed by the enhancement of the leaf pigments content (chlorophyll a and chlorophyll b). This was shown by the positive significant correlations observed between photosynthesis rate with chlorophyll a (r2 = 0.536; p ≤ 0.05) and chlorophyll b (r2 = 0.540; p ≤ 0.05). As the shading levels and time interval increased, the production of total anthocyanin content (TAC) and antioxidant properties of Ferric Reducing Antioxidant Power (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) also increased. The total phenolic content (TPC) and total flavonoid content (TFC) were also significantly enhanced under T2 and T3. The current study suggested that P.minus induce the production of more leaf pigments and secondary metabolites as their special adaptation mechanism under low light condition. Although the biomass was affected under low light, the purpose of conducting the study to boost the bioactive properties in Polygonum minus has been fulfilled by 50% shading under 16 weeks’ exposure.


2013 ◽  
Vol 78 (11) ◽  
pp. 1775-1787 ◽  
Author(s):  
Mihajlo Etinski ◽  
Milena Petkovic ◽  
Miroslav Ristic

Chlorophylls have been extensively investigated both experimentally and theoretically owing to the fact that they are essential for photosynthesis. We have studied two forms of chlorophyll, chlorophyll a and chlorophyll b, by means of density functional theory. Optimization of S0, S1 and T1 states was performed with the B3-LYP functional. The computed fluorescence lifetimes show good agreement with the available experimental data. The electronic adiabatic energies of S1 and T1 states are 2.09/2.12 and 1.19/1.29 eV for chlorophyll a and chlorophyll b respectively. We discussed the implications of this results on the triplet formation. Also, the calculated vertical ionization potentials shows good agreement with the experimental results.


Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 1037-1046 ◽  
Author(s):  
Muhammad Irfan ◽  
Jia-Xing Sun ◽  
Yanbin Liu ◽  
Xue Li ◽  
Shuang Yang

Chlorophyll is an important factor which also affects the yield in maize. In this study, genetic analysis of chlorophyll content was conducted by joint segregation analysis of four generations P1, P2, F1 and F2:4 from the cross Shen3336?Shen3265 using the mixed major genes and polygenes inheritance models. Genetics of chlorophyll revealed that chlorophyll ?a? was controlled by two main gene having additive-dominanceepistasis effects. The heritability of these genes were 56.3%. Chlorophyll ?b? was controlled by two pairs of codominant major gene plus additive-dominance polygene. The heritability of these major genes and polygenes were 1.12% and 93.26% respectively. Chlorophyll ?a+b? was controlled by two pairs of additive-dominance- epistatic major genes plus additive-dominance polygene having heritability of 56.2% and 5.2% respectively.


Sign in / Sign up

Export Citation Format

Share Document