Terminal protection of small-molecule-linked DNA for sensitive fluorescence detection of protein binding based on nucleic acid amplification

The Analyst ◽  
2013 ◽  
Vol 138 (23) ◽  
pp. 7218 ◽  
Author(s):  
Li-Juan Ou ◽  
Hai-Bo Wang ◽  
Xia Chu
Keyword(s):  
Nucleic Acid ◽  
Protein Binding ◽  
Small Molecule ◽  
Fluorescence Detection ◽  
Nucleic Acid Amplification

Fluorescence detection in Lab-on-a-chip systems using ultrafast nucleic acid amplification methods

2014 ◽  
Author(s):  
Rainer Gransee ◽  
Tristan Schneider ◽  
Deniz Elyorgun ◽  
Xenia Strobach ◽  
Tobias Schunck ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Fluorescence Detection ◽  
Lab On A Chip ◽  
Nucleic Acid Amplification

scholarly journals A Guide to Simple and Informative Binding Assays

2010 ◽  
Vol 21 (23) ◽  
pp. 4061-4067 ◽  
Author(s):  
Thomas D. Pollard
Keyword(s):  
Nucleic Acid ◽  
Protein Binding ◽  
Small Molecule ◽  
Protein A ◽  
Hard Work ◽  
Binding Assays

The aim of binding assays is to measure interactions between two molecules, such as a protein binding another protein, a small molecule, or a nucleic acid. Hard work is required to prepare reagents, but flaws in the design of many binding experiments limit the information obtained. In particular many experiments fail to measure the affinity of the reactants for each other. This essay describes simple methods to get the most out of valuable reagents in binding experiments.

Sign in / Sign up

Export Citation Format

Share Document