scholarly journals Low-Cost, Open-Source Device for High-Performance Fluorescence Detection of Isothermal Nucleic Acid Amplification Reactions

2021 ◽  
Author(s):  
Andrew H. Buultjens ◽  
Koen Vandelannoote ◽  
Liam K. Sharkey ◽  
Benjamin P. Howden ◽  
Ian R. Monk ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Open Source ◽  
Fluorescence Detection ◽  
High Performance ◽  
Low Cost ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

scholarly journals A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification

Sensors ◽  
2015 ◽  
Vol 15 (9) ◽  
pp. 23418-23430 ◽  
Author(s):  
Pascal Craw ◽  
Ruth Mackay ◽  
Angel Naveenathayalan ◽  
Chris Hudson ◽  
Manoharanehru Branavan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Low Cost ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

scholarly journals A Robust, Low-Cost Instrument for Real-time Colorimetric Isothermal Nucleic Acid Amplification

2021 ◽  
Author(s):  
Frank Myers ◽  
Brian Moffatt ◽  
Ragheb El Khaja ◽  
Titash Chatterjee ◽  
Gurmeet Marwaha ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Point Of Care ◽  
Low Cost ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Diagnostic Assays ◽  
Isothermal Nucleic Acid Amplification ◽  
Point Of Care Diagnostics ◽  
Time Readout

The COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we present the design and construction of LARI along with thermal, optical, and assay performance characteristics. LARI can be produced for under $1500 and has broad applications in R&D, point-of-care diagnostics, and global health.

scholarly journals Real-time isothermal nucleic acid amplification detection in resource-limited settings: a description of an open-source miniature fluorimeter

2021 ◽  
Vol 9 ◽  
pp. S6
Author(s):  
Jackson Coole ◽  
Alex Kortum ◽  
Yubo Tang ◽  
Imran Vohra ◽  
Mary Natoli ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Open Source ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Resource Limited Settings ◽  
Isothermal Nucleic Acid Amplification ◽  
Resource Limited

scholarly journals Isothermal nucleic acid amplification in bioanalysis

2016 ◽  
Vol 408 (30) ◽  
pp. 8581-8582 ◽  
Author(s):  
María Jesús Lobo-Castañón
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

scholarly journals Quantitative Analysis of Fluorescence Detection Using a Smartphone Camera for a PCR Chip

Sensors ◽  
2021 ◽  
Vol 21 (11) ◽  
pp. 3917
Author(s):  
Jong-Dae Kim ◽  
Chan-Young Park ◽  
Yu-Seop Kim ◽  
Ji-Soo Hwang
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Detection ◽  
High Performance ◽  
Low Cost ◽  
Dna Amplification ◽  
Fluorescent Detection ◽  
Rt Pcr ◽  
Commercial System ◽  
Intensity Changes ◽  
The Difference

Most existing commercial real-time polymerase chain reaction (RT-PCR) instruments are bulky because they contain expensive fluorescent detection sensors or complex optical structures. In this paper, we propose an RT-PCR system using a camera module for smartphones that is an ultra small, high-performance and low-cost sensor for fluorescence detection. The proposed system provides stable DNA amplification. A quantitative analysis of fluorescence intensity changes shows the camera’s performance compared with that of commercial instruments. Changes in the performance between the experiments and the sets were also observed based on the threshold cycle values in a commercial RT-PCR system. The overall difference in the measured threshold cycles between the commercial system and the proposed camera was only 0.76 cycles, verifying the performance of the proposed system. The set calibration even reduced the difference to 0.41 cycles, which was less than the experimental variation in the commercial system, and there was no difference in performance.

A simple isothermal nucleic acid amplification method for the effective on-site identification for adulteration of pork source in mutton

Food Control ◽  
2019 ◽  
Vol 98 ◽  
pp. 297-302 ◽  
Author(s):  
Rui Liu ◽  
Xiudan Wang ◽  
Xuejiao Wang ◽  
Yanjing Shi ◽  
Chao Shi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification ◽  
Amplification Method ◽  
Site Identification

Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing

The Analyst ◽  
2021 ◽  
Author(s):  
Rui Jie Li ◽  
Michael G. Mauk ◽  
Youngung Seok ◽  
Haim H. Bau
Keyword(s):  
Nucleic Acid ◽  
Phase Change ◽  
Phase Change Material ◽  
Heat Generation ◽  
Temperature Regulation ◽  
Nucleic Acid Amplification ◽  
Enzymatic Amplification ◽  
Isothermal Nucleic Acid Amplification ◽  
Home Testing ◽  
Change Material

Electricty-free incubation of isothermal enzymatic amplification with a composite comprised of exothermic reactants for heat generation and phase change material for temperature regulation.

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