A graphene oxide-based immobilized PNGase F reagent for highly efficient N-glycan release and MALDI-TOF MS profiling

2014 ◽  
Vol 6 (8) ◽  
pp. 2518 ◽  
Author(s):  
Xiaojun Ren ◽  
Haihong Bai ◽  
Yiting Pan ◽  
Wei Tong ◽  
Peibin Qin ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Maldi Tof Ms ◽  
Highly Efficient ◽  
Maldi Tof ◽  
Pngase F ◽  
Tof Ms

scholarly journals Enzymatic Digestion and Mass Spectroscopies of N-Linked Glycans in Lacquer Stellacyanin fromRhus vernicifera

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Oyunjargal Tumurbaatar ◽  
Takashi Yoshida
Keyword(s):  
Enzymatic Digestion ◽  
Acetone Powder ◽  
Blue Color ◽  
Sephadex Column ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Glycosylation Sites ◽  
Pngase F ◽  
C 50 ◽  
Tof Ms

Lacquer stellacyanin was isolated and purified from lacquer acetone powder by continuous Sephadex column chromatographies using Sephadex C-50, DEAE A-50, and C-50 gels. The purified lacquer stellacyanin had a blue color with one major and three minor bands around 26 k Dain SDS PAGE. Trypsin- and chymotrypsin-treated lacquer stellacyanins were examined by LC/MS/MS to determine three N-glycosylation sites (N28, N60, and N102) and were further analyzed by MALDI TOF MS, indicating that the N-linked glycans were attached to the three asparagine (Asn) sites, respectively. In addition, after trypsin digestion and PNGase A and PNGase F treatments to cleave N-linked glycans from the Asn sites, it was found that lacquer stellacyanin had a xylose containing a biantennary N-linked glycan with core fucosylation consisting of 13 sugar residues (a complex type N-linked glycan) by MALDI TOF MS analysis. This is the first report on the structure of an N-linked glycan in lacquer stellacyanin.

scholarly journals Reduced graphene oxide films used as matrix of MALDI-TOF-MS for detection of octachlorodibenzo-p-dioxin

2010 ◽  
Vol 46 (37) ◽  
pp. 6974 ◽  
Author(s):  
Xiaozhu Zhou ◽  
Yanyan Wei ◽  
Qiyuan He ◽  
Freddy Boey ◽  
Qichun Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Oxide Films ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Reduced Graphene ◽  
Tof Ms
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