Modulation of the pharmacokinetics of zinc oxide nanoparticles and their fates in vivo

Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents.

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Antibacterial Ferroelectric Hybrid Membranes Fabricated via Electrospinning for Wound Healing

Membranes ◽

10.3390/membranes11120986 ◽

2021 ◽

Vol 11 (12) ◽

pp. 986

Author(s):

Ivan V. Lukiev ◽

Ludmila S. Antipina ◽

Semen I. Goreninskii ◽

Tamara S. Tverdokhlebova ◽

Dmitry V. Vasilchenko ◽

...

Keyword(s):

Wound Healing ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Vinylidene Fluoride ◽

Antibacterial Properties ◽

Oxide Nanoparticles ◽

Hybrid Membranes ◽

Electrospinning Technique ◽

Β Phase

In the present study, wound healing ferroelectric membranes doped with zinc oxide nanoparticles were fabricated from vinylidene fluoride-tetrafluoroethylene copolymer and polyvinylpyrrolidone using the electrospinning technique. Five different ratios of vinylidene fluoride-tetrafluoroethylene to polyvinylpyrrolidone were used to control the properties of the membranes at a constant zinc oxide nanoparticle content. It was found that an increase of polyvinylpyrrolidone content leads to a decrease of the spinning solution conductivity and viscosity, causing a decrease of the average fiber diameter and reducing their strength and elongation. By means of X-ray diffraction and infrared spectroscopy, it was revealed that increased polyvinylpyrrolidone content leads to difficulty in crystallization of the vinylidene fluoride-tetrafluoroethylene copolymer in the ferroelectric β-phase in membranes. Changing the ratio of vinylidene fluoride-tetrafluoroethylene copolymer and polyvinylpyrrolidone with a constant content of zinc oxide nanoparticles is an effective approach to control the antibacterial properties of membranes towards Staphylococcus aureus. After carrying out in vivo experiments, we found that ferroelectric hybrid membranes, containing from five to ten mass percent of PVP, have the greatest wound-healing effect for the healing of purulent wounds.

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Alteration of gene expression by zinc oxide nanoparticles or zinc sulfate in vivo and comparison with in vitro data: A harmonious case

Theriogenology ◽

10.1016/j.theriogenology.2016.03.006 ◽

2016 ◽

Vol 86 (3) ◽

pp. 850-861.e1 ◽

Cited By ~ 11

Author(s):

Wei-Dong Zhang ◽

Yong Zhao ◽

Hong-Fu Zhang ◽

Shu-Kun Wang ◽

Zhi-Hui Hao ◽

...

Keyword(s):

Gene Expression ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Zinc Sulfate ◽

Oxide Nanoparticles ◽

Vitro Data ◽

In Vitro Data

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In vivo zinc oxide nanoparticles induces acute hyperglycemic response a dose-dependent and route of administration in healthy and diabetic rats

10.3762/bxiv.2019.75.v1 ◽

2019 ◽

Author(s):

Jesús José Ibarra-Leal ◽

Luis Yocupicio ◽

Alejandro Apolinar-Iribe ◽

Irene Díaz-Reval ◽

Hortensia Parra-Delgado ◽

...

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Experimental Diabetes ◽

Intraperitoneal Administration ◽

Diabetic Rats ◽

Oxide Nanoparticles ◽

Acute Effects ◽

Fasting Glycemia ◽

Dose Dependent

In recent years, different studies carried out in experimental diabetes models suggest that zinc oxide nanoparticles (ZnONPs) can be good antidiabetic agents, this evidence was obtained from long-term treatments using repeated doses of zinc oxide nanoparticles. The goal of this work was to evaluate the acute effects during six hours post-administration of ZnONPs on glycemia in healthy and diabetic rats and to compare the effects of oral and intraperitoneal administration. For this study male Wistar rats were used and experimental diabetes was induced by streptozotocin-nicotinamide intraperitoneal administration. At short-term ZnONPs administration induced hyperglycemic response in healthy and diabetic rats, the effect was dose-dependent and administration route. The diabetic rats were more sensitive to ZnONPs effect. In conclusion, this study provides novel information about the acute effects of zinc oxide nanoparticles on the fasting glycemia in experimental diabetes in vivo model and healthy rats, these data are important for its future clinical applications.

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