Multiplexed determination of human growth hormone and prolactin at a label free electrochemical immunosensor using dual carbon nanotube–screen printed electrodes modified with gold and PEDOT nanoparticles

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4556-4563 ◽  
Author(s):  
V. Serafín ◽  
G. Martínez-García ◽  
L. Agüí ◽  
P. Yáñez-Sedeño ◽  
J. M. Pingarrón
Keyword(s):  
Growth Hormone ◽  
Carbon Nanotube ◽  
Simultaneous Determination ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Screen Printed Electrodes ◽  
First Time

A label-free dual electrochemical immunosensor for simultaneous determination of human growth and prolactin hormones was prepared for the first time.

scholarly journals ELISA for Determination of Human Growth Hormone: Recognition of Helix 4 Epitopes

2004 ◽  
Vol 2004 (3) ◽  
pp. 143-149 ◽  
Author(s):  
Juliana F. Moura ◽  
Luiz DeLacerda ◽  
Romolo Sandrini ◽  
Fernanda M. Borba ◽  
Denise N. Castelo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Synthetic Peptide ◽  
Human Growth ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoaffinity Column ◽  
Receptor Dimerization ◽  
Human Prolactin

Human growth hormone (hGH) signal transduction initiates with a receptor dimerization in which one molecule binds to the receptor through sites 1 and 2. A sandwich enzyme-linked immunosorbent assay was developed for quantifying hGH molecules that present helix 4 from binding site 1. For this, horse anti-rhGH antibodies were eluted by an immunoaffinity column constituted by sepharose-rhGH. These antibodies were purified through a second column with synthetic peptide correspondent tohGH helix 4, immobilized to sepharose, and used as capture antibodies. Those that did not recognize synthetic peptide were used as a marker antibody. The working range was of 1.95 to 31.25 ng/mL of hGH. The intra-assay coefficient of variation (CV) was between 4.53% and 6.33%, while the interassay CV was between 6.00% and 8.27%. The recovery range was between 96.0% to 103.8%. There was no cross-reactivity with human prolactin. These features show that our assay is an efficient method for the determination of hGH.

Carbon-nanotube-modified screen-printed electrodes, a cationic surfactant, and a peak deconvolution procedure: alternatives to provide satisfactory simultaneous determination of three synthetic antioxidants in complex samples

2015 ◽  
Vol 7 (9) ◽  
pp. 3764-3771 ◽  
Author(s):  
Ricardo Pini Caramit ◽  
Alessandra Silveira Antunes Araújo ◽  
Daniela Kárin Fogliatto ◽  
Luiz Henrique Viana ◽  
Magno Aparecido Gonçalves Trindade ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Simultaneous Determination ◽  
Cationic Surfactant ◽  
Synthetic Antioxidants ◽  
Complex Samples ◽  
Peak Deconvolution ◽  
Screen Printed Electrodes ◽  
Alternative Approach ◽  
Screen Printed

An alternative approach to allow simultaneous determination of three synthetic antioxidants has been developed.

Determination of antibodies against human growth hormone using a direct immunoassay format and different electrochemical methods

The Analyst ◽  
2013 ◽  
Vol 138 (5) ◽  
pp. 1427 ◽  
Author(s):  
Natalija German ◽  
Asta Kausaite-Minkstimiene ◽  
Justina Kirlyte ◽  
Asta Makaraviciute ◽  
Arunas Ramanavicius ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Electrochemical Methods ◽  
Direct Immunoassay

scholarly journals A new voltammetric method for the simultaneous determination of the antioxidants TBHQ and BHA in biodiesel using multi-walled carbon nanotube screen-printed electrodes

Fuel ◽  
2013 ◽  
Vol 105 ◽  
pp. 306-313 ◽  
Author(s):  
Ricardo Pini Caramit ◽  
Adriana Garabini de Freitas Andrade ◽  
João Batista Gomes de Souza ◽  
Tatiane Alfonso de Araujo ◽  
Luiz Henrique Viana ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Simultaneous Determination ◽  
Voltammetric Method ◽  
Screen Printed Electrodes ◽  
Multi Walled Carbon Nanotube ◽  
Screen Printed

scholarly journals UHPLC-HRMS determination of some human growth hormone-releasing peptides in urine

2019 ◽  
Vol 23 (4) ◽  
pp. 509-516
Author(s):  
A. Z. Temerdashev ◽  
◽  
E. V. Dmitrieva ◽  
A. A. Azaryan ◽  
D. A. Burmikin ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Growth Hormone Releasing Peptides
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