A recyclable chitosan-based QCM biosensor for sensitive and selective detection of breast cancer cells in real time

The Analyst ◽  
2014 ◽  
Vol 139 (23) ◽  
pp. 6259-6265 ◽  
Author(s):  
Shaolian Zhang ◽  
Haihua Bai ◽  
Jinmei Luo ◽  
Peihui Yang ◽  
Jiye Cai
Keyword(s):  
Breast Cancer ◽  
Folic Acid ◽  
Cancer Cells ◽  
Real Time ◽  
Breast Cancer Cells ◽  
Time Measurement ◽  
Selective Detection ◽  
Real Time Measurement ◽  
First Time ◽  
Mcf 7

A sensitive and recyclable QCM biosensor for the real-time measurement of MCF-7 breast cancer cells was developed for the first time using folic acid coupled to chitosan as an excellent biocompatible biosensor film.

Synthesis and characterization of NaYF4:Yb3+,Er3+@silica-N=folic acid nanocomplex for bioimaginable detecting MCF-7 breast cancer cells

2019 ◽  
Vol 37 (11) ◽  
pp. 1183-1187 ◽  
Author(s):  
Ha Thi Phuong ◽  
Tran Thu Huong ◽  
Le Thi Vinh ◽  
Hoang Thi Khuyen ◽  
Do Thi Thao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Folic Acid ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Synthesis And Characterization ◽  
Mcf 7

scholarly journals The role of vulpinic acid as a natural compound in the regulation of breast cancer-associated miRNAs

2021 ◽  
Vol 54 (1) ◽  
Author(s):  
Demet Cansaran-Duman ◽  
Sevcan Yangın ◽  
Betül Çolak
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Real Time ◽  
Microarray Analysis ◽  
Mirna Expression ◽  
Breast Cancer Cells ◽  
Mcf 7

Abstract Background Breast cancer is the most frequently diagnosed cancer, and no effective treatment solution has yet been found. The number of studies based on the research of novel natural compounds in the treatment of breast cancer has been increasing in recent years. The anticancer properties of natural compounds are related to the regulation of microRNA (miRNA) expression. Therefore, changing the profile of miRNAs with the use of natural products is very important in cancer treatment. However, the role of vulpinic acid and related miRNAs in breast cancer progression remains unknown. Vulpinic acid, methyl (as2E)-2-(3-hydroxy-5-oxo-4-phenylfuran-2-ylidene)-2 phenylacetate, is a natural product extracted from the lichen species and shows an anticancer effect on different cancer cells. Methods This study examines the effects of vulpinic acid on the miRNA levels of breast cancer (MCF-7) cells and its relationship with cell proliferation and apoptosis levels. The antiproliferative effect of vulpinic acid was screened against MCF-7 breast cancer cells and MCF-12A breast epithelial cells using the xCELLigence real-time cell analysis system. We analyzed the altered miRNA expression profile in MCF-7 breast cancer cells versus MCF-12A cells following their response to vulpinic acid through microarray analysis. The microarray analysis results were confirmed through quantitative real-time PCR and bioinformatics analysis. Results The results of the miRNA array and bioinformatic analyses demonstrated that 12 miRNAs were specifically responsive to vulpinic acid in MCF-7 breast cancer cells. This is the first study to reveal that vulpinic acid inhibits the expression of 12 miRNAs and suppresses breast cancer cell proliferation. The study also revealed that vulpinic acid may downregulate the expression of 12 miRNAs by repressing the FOXO-3 gene. The miRNA targets were mainly found to play a role in the apoptosis, cell cycle and MAPK pathways. Moreover, Bcl-2, Bax, procaspase-3 and procaspase-9 protein levels were assessed by western blot analysis for validation of apoptosis at the protein level. Conclusion This study revealed the molecular mechanisms of vulpinic acid on breast cancer and showed that vulpinic acid regulates apoptosis signaling pathways by decreasing the expression of miRNAs. The miRNA expression patterns illuminate the underlying effect of vulpinic acid in breast cancer treatment. Graphical Abstract

Bioenergetic differences between MCF-7 and T47D breast cancer cells and their regulation by oestradiol and tamoxifen

2014 ◽  
Vol 465 (1) ◽  
pp. 49-61 ◽  
Author(s):  
Brandie N. Radde ◽  
Margarita M. Ivanova ◽  
Huy Xuan Mai ◽  
Joshua K. Salabei ◽  
Bradford G. Hill ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Real Time ◽  
Oestrogen Receptor ◽  
Breast Cancer Cells ◽  
Flux Analysis ◽  
Intact Cells ◽  
Extracellular Flux ◽  
Oestrogen Receptor Α ◽  
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Oestrogen receptor α-expressing breast cancer cells show differences in basal bioenergetics profiles and bioenergetics responses to serum depletion, oestradiol and tamoxifen as measured in real time by extracellular flux analysis in intact cells.

Design, In Silico Modelling, and Functionality Theory of Novel Folate Receptor Targeted Rutin Encapsulated Folic Acid Conjugated Keratin Nanoparticles for Effective Cancer Treatment

2020 ◽  
Vol 19 (16) ◽  
pp. 1966-1982 ◽  
Author(s):  
Selvaraj Kunjiappan ◽  
Theivendren Panneerselvam ◽  
Saravanan Govindaraj ◽  
Pavadai Parasuraman ◽  
Suraj Baskararaj ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Cell Death ◽  
Folic Acid ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Folate Receptor ◽  
Release Kinetics ◽  
Covalent Binding ◽  
Mcf 7

Objective: Site-specific and toxic-free drug delivery, is an interesting area of research. Nanoengineered drug delivery systems possess a remarkable potential for effective treatment of various types of cancers. Methods: In this study, novel Folic Acid (FA) conjugated keratin nanoparticles (NPs) were assembled with encapsulation and delivery of Rutin (Rt) into breast cancer cells through the overexpressed folate receptor. The biocompatible, Rt encapsulated FA conjugated keratin NPs (FA@Ker NPs) were successfully formulated by a modified precipitation technique. Their morphological shape and size, size distribution, stability, and physical nature were characterized and confirmed. The drug (Rt) encapsulation efficiency, loading capacity and release kinetics were also studied. Results: The observed results of molecular docking and density functionality theory of active drug (Rt) showed a strong interaction and non-covalent binding of the folate receptor and facilitation of endocytosis in breast cancer cells. Further, in vitro cytotoxic effect of FA@Ker NPs was screened against MCF-7 cancer cells, at 55.2 µg/mL of NPs and found to display 50% of cell death at 24h. Moreover, the NPs enhanced the uptake of Rt in MCF-7 cells, and the apoptotic effect of condensed nuclei and distorted membrane bodies was observed. Also, NPs entered into the mitochondria of MCF-7 cells and significantly increased the level of ROS which led to cell death. Conclusion: The developed FA@Ker NPs might be a promising way to enhance anti-cancer activity without disturbing normal healthy cells.

Periplaneta Americana Extract Inhibits Proliferation and Motility of Human Breast Cancer Cells Through MAPK/ERK Signaling Pathway and Promotes Cell Apoptosis

2020 ◽  
Vol 19 (2) ◽  
pp. 194-198
Author(s):  
Xiaoying Wu ◽  
Zhe Sun ◽  
Bailing Shi ◽  
Xiaoli Zhang
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Breast Cancer Cells ◽  
Periplaneta Americana ◽  
Erk Signaling ◽  
Antitumor Activities ◽  
Cancer Multiple ◽  
First Time ◽  
Mcf 7

Breast cancer is the most common malignant tumor among women, and its incidence is second only to cervical cancer in China. The search for natural products with antitumor activity is a potentially appealing strategy for the treatment of breast cancer. Multiple studies have shown that extracts of Periplaneta americana exhibit antitumor activities. However, there is no study on the effect of P. americana extracts on breast cancer. We have shown here for the first time that the extracts of P. americana inhibit survival of MCF-7 breast cancer cells via interference with MAPK/ERK signaling pathway.

Upconversion rare earth nanoparticles functionalized with folic acid for bioimaging of MCF-7 breast cancer cells

2017 ◽  
Vol 33 (2) ◽  
pp. 191-200 ◽  
Author(s):  
Dalia Chávez-García ◽  
Karla Juárez-Moreno ◽  
Cristian H. Campos ◽  
Joel B. Alderete ◽  
Gustavo A. Hirata
Keyword(s):  
Breast Cancer ◽  
Rare Earth ◽  
Folic Acid ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Image Position ◽  
Mcf 7 ◽  
Rare Earth Nanoparticles

Abstract

scholarly journals Development and characterization of folic acid-functionalized apoferritin as a delivery vehicle for epirubicin against MCF-7 breast cancer cells

2018 ◽  
Vol 46 (sup3) ◽  
pp. S847-S854 ◽  
Author(s):  
Hasanain Gomhor J. Alqaraghuli ◽  
Soheila Kashanian ◽  
Ronak Rafipour ◽  
Elahe Mahdavian ◽  
Kamran Mansouri
Keyword(s):  
Breast Cancer ◽  
Folic Acid ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Delivery Vehicle ◽  
Mcf 7

scholarly journals Flavanoids fromthe Stembark of Chisocheton pentandrus(Meliaceae)

2017 ◽  
pp. 122-126
Author(s):  
Supriatno Supriatno ◽  
Ace Tatang Hidayat ◽  
Kindi Farabi ◽  
Fajar Fauzi Abdullah ◽  
Nurlelasari Nurlelasari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Spectral Data ◽  
Breast Cancer Cells ◽  
Chemical Structure ◽  
First Time ◽  
Mcf 7

Two flavanoid compounds, catechin (1) and epicatechin (2), have been isolated from the stembark of Chisocheton pentandrus. The chemical structure of compounds1and2were identified byspectroscopic data including, UV, IR, NMR (1H, 13C, DEPT 135°, HMQC, HMBC, 1H-1H COSY) and MS and by comparing with previously reported spectral data. Compounds 1 and 2, were isolated in this plant for first time and showed no cytotoxic activity against MCF-7 breast cancer cells.

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