Flavanoids fromthe Stembark of Chisocheton pentandrus(Meliaceae)

Two flavanoid compounds, catechin (1) and epicatechin (2), have been isolated from the stembark of Chisocheton pentandrus. The chemical structure of compounds1and2were identified byspectroscopic data including, UV, IR, NMR (1H, 13C, DEPT 135°, HMQC, HMBC, 1H-1H COSY) and MS and by comparing with previously reported spectral data. Compounds 1 and 2, were isolated in this plant for first time and showed no cytotoxic activity against MCF-7 breast cancer cells.

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Triterpenoids from The Bark of Garcinia porecta and their Cytotoxic Activity against MCF7 Breast Cancer Lines

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.15.1.20262.1-9 ◽

2019 ◽

Vol 15 (1) ◽

pp. 1

Author(s):

Darwati Darwati ◽

Alya Tsamrotul ◽

Tati Herlina ◽

Tri Mayanti ◽

Nurlelasari Nurlelasari ◽

...

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Structure Elucidation ◽

Breast Cancer Cells ◽

Chemical Structure ◽

Spectrometric Analysis ◽

Tropical Plant ◽

Phytochemical Studies ◽

First Time ◽

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The Garcinia genus is a well known tropical plant in the Indo-Malesiana region and mainly distributed in tropical countries including Indonesia, Thailand, and Malaysia. Previous phytochemical studies on Garcinia species have led to the identification and isolation of mainly prenylated xanthones. This research describes the isolation and structure elucidation of isolated triterpenoids compounds from the bark of Garcinia porecta. Dried powder bark of G. porecta was extracted with methanol and then partitioned with n-hexane, ethyl acetate, and n-butanol. The n-hexane extract then was separated and purified with chromatography techniques to obtain isolated compounds 1 and 2. The chemical structure of isolated compounds was elucidated by spectroscopic methods including one and two-dimensional NMR as well as high-resolution mass spectrometric analysis and identified as lanosterol (1) dan arabidiol (2), respectively. These triterpenoids were isolated from this plant for the first time. Compound 1 and 2 showed weak cytotoxic activity against MCF-7 breast cancer cells with IC50 values of 60.09 dan 46.17 µM, respectively.

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Structural and In Vitro Biological Studies of Organotin(IV) Precursors; Selective Inhibitory Activity Against Human Breast Cancer Cells, Positive to Estrogen Receptors

Australian Journal of Chemistry ◽

10.1071/ch12448 ◽

2012 ◽

Vol 65 (12) ◽

pp. 1625 ◽

Cited By ~ 25

Author(s):

Vasilis I. Balas ◽

Christina N. Banti ◽

Nikolaos Kourkoumelis ◽

Sotiris K. Hadjikakou ◽

George D. Geromichalos ◽

...

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Cell Line ◽

Breast Cancer Cells ◽

X Ray ◽

Er Positive ◽

Normal Human ◽

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Crystals of Ph3SnCl (1) were grown from a methanol/acetonitrile solution. Compounds [Ph3SnOH]n (2) and [(Ph2Sn)4Cl2O2(OH)2] (3) were crystallized from diethyl ether/methanol/acetonitrile and hot acetone/water solutions respectively, of the white precipitation, formed by adding KOH to solutions of 1 and [Ph2SnCl2] in 1 : 1 and 1 : 2 molar ratios respectively. Complex 1 was characterized by X-ray crystallography. X-ray structure determination of compounds 2 and 3 confirmed the previously reported identities. The molecular structure of 1, reported here, is a new polymorphic form of the known one for Ph3SnCl. Four independent [Ph3SnCl] molecules constitute the crystal structure of 1. The moieties are packed in two pairs in a tail-to-tail arrangement. Complexes 1–3 were evaluated for their in vitro cytotoxic activity (cell viability) against human cancer cell lines: HeLa (human cervical), MCF-7 (breast, estrogen receptor (ER) positive), MDA-MB-231 (breast, ER negative), A549 (lung), Caki-1 (kidney carcinoma), 786-O (renal adenocarcinoma), K1 (thyroid carcinoma), and the normal human lung cell line MRC-5 (normal human fetal lung fibroblast cells) versus, the normal immortalized human mammary gland epithelial cell line MTSV17 with a sulforhodamine B (SRB) assay. The results show potent cytotoxic activity of the complexes against all cell lines used, which was superior to that of cisplatin (CDDP). Compounds 1–3 showed higher activity against breast cancer cells MCF-7 (ER positive) than against of MDA-MB-231 (ER negative). These findings prompted us to search for possible interaction of these complexes with other cellular elements of fundamental importance in cell proliferation. The influence of these complexes 1–3 upon the catalytic peroxidation of linoleic acid to hydroperoxylinoleic acid by the enzyme lipoxygenase (LOX), as well as their binding affinity towards calf thymus-DNA, were kinetically and theoretically studied.

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Caryophyllene-Type Sesquiterpenoids from the Stembark of Aglalia harmsiana and Their Cytotoxic Activity Against MCF-7 Breast Cancer Cells

Molekul ◽

10.20884/1.jm.2019.14.2.543 ◽

2019 ◽

Vol 14 (2) ◽

pp. 126 ◽

Cited By ~ 1

Author(s):

Hersa Milawati ◽

Desi Harneti ◽

Rani Maharani ◽

Nurlelasari Nurlelasari ◽

Ace Tatang Hidayat ◽

...

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Spectroscopic Methods ◽

Caryophyllene Oxide ◽

Tropical Plants ◽

Chemical Structures ◽

Hexane Extracts ◽

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Sesquiterpenoid is a class of terpenoid compounds that have the most abundant diversity of structures and biological activitiesthat can be found in natural resources. Tropical plants are main source of sesquiterpenoid compounds such as Aglaiagenus belong to Meliaceae family. A. harmsianais a species from Aglaiathat only has few previous researchs. Therefore, the purpose of this study was to isolate and determine the structure of sesquiterpenoid compounds from stem barkA. harmsianaalong withtheir cytotoxic activity against MCF-7 breast cancer cells. The isolation process begins by extracting powder from A. harmsianastembark using n-hexane, ethyl acetate and methanol. All extracts were evaluated for their cytotoxic activity against MCF-7 breast cancer cells, and n-hexane extractsshowed significant cytotoxic activitywith IC50values of 117.86 µg/mL. Therefore, n-hexane extracts were further separated and purified by various chromatographic techniquesto obtain compounds 1and 2. Compounds 1and 2were elucidated their chemical structures by spectroscopic methods includingIR, NMR, and MS as well as bycomparison of data with literatures and identified ascaryophyllene-typesesquiterpenoids, β-caryophylleneoxide (1) and senecrassidiol (2). Compounds 1and 2were submitted for cytotoxic eveluation on MCF-7 breast cancer cells and as a result, β-caryophyllene oxide (1) showed the stronger activity compared to senecrassidiol (2). These finding indicatedthat the cytotoxic activity of caryophyllene-typesesquiterpenoid areinfluenced by the presence of double bonds and configuration of methyl groups.

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CYTOTOXIC ACTIVITY AND SELECTIVITY INDEX OF BINAHONG (Anredera cordifolia) EXTRACTS ON MCF-7 BREAST CANCER CELLS AND VERO NORMAL CELLS LINE

Acta Biochimica Indonesiana ◽

10.32889/actabioina.v3i2.57 ◽

2021 ◽

Vol 3 (2) ◽

pp. 72-80

Author(s):

Dita Sozianty ◽

Rifki Febriansah

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Selectivity Index ◽

Cytotoxic Agents ◽

Flavonoid Compounds ◽

Ic50 Value ◽

Her 2 ◽

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Background: Breast cancers occur because of an impaired balance between proliferation, differentiation, and apoptosis of breast glands. Natural products have potency as cytotoxic agents with less side effects than chemotherapy. One of the potential plants is Anredera cordifolia (Ten.) Stennis (binahong), which contains flavonoid 8-glucopyranosyl- 4’,5,7-trihydroxy flavone compounds.Objective: This study aims to determine the potency of binahong leaves extract as an anticancer for breast cancer in vitro and in silico.Methods: Preliminary tests using molecular docking of 8-glucopyranosyl-4’,5,7-trihydroxyflavone compounds on Bcl-2 and HER-2 proteins. The extraction and fractionation were to obtain binahong extract. Thin layer chromatography to identify flavonoid compounds in the extract. DPPH assay was performed to evaluate the antioxidant activity. MTT assay was performed to evaluate cytotoxic activity on MCF-7 breast cancer cells and Vero cells.Results: In silico test showed a stable bond between 8-glucopyranosyl- 4’,5,7-trihydroxylflavone, and Bcl-2 and HER-2 with a docking score of -7.5 kcal/mol and -8.0 kcal/mol, respectively. The binahong extract contain flavonoid compounds that had the retention factor value 0.78; 0.49; 0.35. Antioxidant test resulted IC50 value of 4940 μg/mL. Cytotoxic test resulted in IC50 value of 1073 μg/mL and 486 μg/mL for Vero cells and MCF-7 breast cancer cells, respectively. The comparison between IC50 produced a selectivity index value of 2.149, which shows that binahong extract was selective against MCF-7 breast cancers.Conclusion: This study concluded that binahong extract has weak potency as anticancer agent on MCF-7 cells.

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The Enhanced Cytotoxic and Pro-Apoptotic Effects of Optimized Simvastatin-Loaded Emulsomes on MCF-7 Breast Cancer Cells

Pharmaceutics ◽

10.3390/pharmaceutics12070597 ◽

2020 ◽

Vol 12 (7) ◽

pp. 597 ◽

Cited By ~ 1

Author(s):

Zuhier A. Awan ◽

Usama A. Fahmy ◽

Shaimaa M. Badr-Eldin ◽

Tarek S. Ibrahim ◽

Hani Z. Asfour ◽

...

Keyword(s):

Breast Cancer ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Spherical Shape ◽

Cytotoxic Activities ◽

Vesicle Size ◽

Apoptotic Activity ◽

The Impact ◽

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Statins, including simvastatin (SMV), are commonly used for the control of hyperlipidaemia and have also proven therapeutic and preventative effects in cardiovascular diseases. Besides that, there is an emerging interest in their use as antineoplastic drugs as demonstrated by different studies showing their cytotoxic activity against different cancer cells. In this study, SMV-loaded emulsomes (SMV-EMLs) were formulated and evaluated for their cytotoxic activity in MCF-7 breast cancer cells. The emulsomes were prepared using a modified thin-film hydration technique. A Box–Behnken model was used to investigate the impact of formulation conditions on vesicle size and drug entrapment. The optimized formulation showed a spherical shape with a vesicle size of 112.42 ± 2.1 nm and an entrapment efficiency of 94.34 ± 1.11%. Assessment of cytotoxic activities indicated that the optimized SMV-EMLs formula exhibited significantly lower half maximal inhibitory concentration (IC50) against MCF-7 cells. Cell cycle analysis indicated the accumulation of cells in the G2-M phase as well as increased cell fraction in the pre-G1 phase, suggesting an enhancement of anti-apoptotic activity of SMV. The staining of cells with Annex V revealed an increase in early and late apoptosis, in line with the increased cellular content of caspase-3 and Bax. In addition, the mitochondrial membrane potential (MMP) was significantly decreased. In conclusion, SMV-EMLs demonstrated superior cell death-inducing activity against MCF-7 cells compared to pure SMV. This is mediated, at least in part, by enhanced pro-apoptotic activity and MMP modulation of SMV.

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Probing the interaction of thionine with human serum albumin by multispectroscopic studies and its in vitro cytotoxic activity toward MCF-7 breast cancer cells

Journal of Biomolecular Structure and Dynamics ◽

10.1080/07391102.2016.1235513 ◽

2016 ◽

Vol 35 (14) ◽

pp. 3012-3031 ◽

Cited By ~ 5

Author(s):

Perumal Manivel ◽

Manickam Paulpandi ◽

Kadarkarai Murugan ◽

Giovanni Benelli ◽

Malaichamy Ilanchelian

Keyword(s):

Breast Cancer ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Breast Cancer Cells ◽

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Evaluation of the Cytotoxic Activity of Anthriscus nemorosa on Breast Cancer Cells

Journal of Contemporary Medical Sciences ◽

10.22317/jcms.v7i2.940 ◽

2021 ◽

Vol 7 (2) ◽

Author(s):

Mahtab Zarchini ◽

Haleh Forouhandeh ◽

Elham Safarzadeh ◽

Omoleila Molavi ◽

Vahideh Tarhriz ◽

...

Keyword(s):

Breast Cancer ◽

Flow Cytometry ◽

Cell Death ◽

Cytotoxic Activity ◽

Cancer Cells ◽

Cell Lines ◽

Breast Cancer Cells ◽

Hexane Extract ◽

Volatile Components ◽

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Objective: The genus Anthriscus from the Umbelliferae family has valuable compounds and pharmacological properties. Terpenoids, phenolics, anthocyanins, podophyllotoxins, and others have been identified in Anthriscus genus which has effects like analgesic, antiviral, anti-inflammatory, hepatoprotective, and anti-platelet aggregation. The present study concerns the cytotoxic activity of A. nemorosa different extracts on breast cancer cells (MCF-7) and normal cell lines (HFFF). Methods: Different extracts of aerial parts of A. nemorosa were prepared using Soxhlet apparatus. The cytotoxicity of samples was assessed by MTT assay on breast cancer cells (MCF-7) and noncancerous cells (HFFF) with different concentrations of extracts in 24 and 48 hours. The most potent extract was fractioned and cytotoxic activity of fractions was considered, As well. A flow cytometry (annexin V/PI) assay has been used for detecting the mechanism of cell death in sample treated cell lines. Moreover, for clarifying volatile components of n-Hexane extract and its 80% and 100% VLC fractions were subjected to GC-MS apparatus. Results: Results indicated that n-Hexane extract and its 80% and 100% VLC fractions exhibited a significant (p<0.001) inhibitory effect on the growth of the MCF-7 cell line compared to the control group. Meanwhile, flow cytometry analysis revealed that potent extract caused cell death through necrosis and 80% and 100% fractions showed different mechanisms (such as autophagy). The major compounds, which maybe were in charge of showing cytotoxic activity were non-terpenoids. Conclusion: This study provides the evidence that in vitro cytotoxic activity of n-Hexane extract and 80% and 100% VLC fractions of A. nemorosa inhibited the proliferation of breast cancer cells (MCF7) via a different mechanism.

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Periplaneta Americana Extract Inhibits Proliferation and Motility of Human Breast Cancer Cells Through MAPK/ERK Signaling Pathway and Promotes Cell Apoptosis

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.19:194-198 ◽

2020 ◽

Vol 19 (2) ◽

pp. 194-198

Author(s):

Xiaoying Wu ◽

Zhe Sun ◽

Bailing Shi ◽

Xiaoli Zhang

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Signaling Pathway ◽

Breast Cancer Cells ◽

Periplaneta Americana ◽

Erk Signaling ◽

Antitumor Activities ◽

Cancer Multiple ◽

First Time ◽

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Breast cancer is the most common malignant tumor among women, and its incidence is second only to cervical cancer in China. The search for natural products with antitumor activity is a potentially appealing strategy for the treatment of breast cancer. Multiple studies have shown that extracts of Periplaneta americana exhibit antitumor activities. However, there is no study on the effect of P. americana extracts on breast cancer. We have shown here for the first time that the extracts of P. americana inhibit survival of MCF-7 breast cancer cells via interference with MAPK/ERK signaling pathway.

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