Pomegranate phenolics inhibit formation of advanced glycation endproducts by scavenging reactive carbonyl species

2014 ◽  
Vol 5 (11) ◽  
pp. 2996-3004 ◽  
Author(s):  
Weixi Liu ◽  
Hang Ma ◽  
Leslie Frost ◽  
Tao Yuan ◽  
Joel A. Dain ◽  
...  
Keyword(s):  
Advanced Glycation Endproducts ◽  
Inhibitory Effects ◽  
Advanced Glycation ◽  
Glycation Endproducts ◽  
Reactive Carbonyl Species ◽  
First Time ◽  
Carbonyl Species ◽  
Reactive Carbonyl

The present study reported for the first time the inhibitory effects of pomegranate phenolics on the formation of advanced glycation endproducts and their carbonyl scavenger reactivity.

scholarly journals Assay of advanced glycation endproducts (AGEs): surveying AGEs by chromatographic assay with derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to N∊-carboxymethyl-lysine- and N∊-(1-carboxyethyl)lysine-modified albumin

2002 ◽  
Vol 364 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Naila AHMED ◽  
Ognian K. ARGIROV ◽  
Harjit S. MINHAS ◽  
Carlos A.A. CORDEIRO ◽  
Paul J. THORNALLEY
Keyword(s):  
Advanced Glycation Endproducts ◽  
Advanced Glycation ◽  
Structural Isomers ◽  
Glycation Endproducts ◽  
Assay Procedure ◽  
Carboxymethyl Lysine ◽  
Glycation Process ◽  
First Time ◽  
Hydrolysis Of

Glycation of proteins leads to the formation of early glycation adducts (fructosamine derivatives) and advanced glycation endproducts (AGEs). Formation of AGEs has been linked to the development of cataract, diabetic complications, uraemia, Alzheimer's disease and other disorders. AGEs are a group of compounds of diverse molecular structure and biological function. To characterize AGE-modified proteins used in studies of structural and functional effects of glycation, an assay was developed that surveys the content of early and advanced glycation adducts in proteins. The assay procedure involved enzymic hydrolysis of protein substrate, derivatization of the hydrolysate with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) and HPLC of the resulting adducts with fluorimetric detection. Structural isomers of methylglyoxal-derived hydroimidazolone, glyoxal-derived hydroimidazolone, 3-deoxyglucosone-derived hydroimidazolone and Nδ-(4-carboxy-4,6-dimethyl-5,6-dihydroxy-1,4,5,6-tetrahydropyrimidin-2-yl)-ornithine (THP) were determined for the first time. AGEs with intrinsic fluorescence (argpyrimidine, pentosidine) were assayed without derivatization. Limits of detection were 2–17pmol and levels of recovery were 50–99%, depending on the analyte. The AQC assay resolved structural and epimeric isomers of methylglyoxal-derived hydroimidazolones and THP. Hydroimidazolones, THP and argpyrimidine were AGEs of short-to-intermediate stability under physiological conditions, with half-lives of 1–2weeks. Their measurement provides further insight into the glycation process. The assay was applied to the characterization of human serum albumin minimally and highly modified by N∊-carboxymethyl-lysine and N∊-(1-carboxyethyl)-lysine.

scholarly journals Reactive Metabolites and AGE-RAGE-Mediated Inflammation in Patients following Liver Transplantation

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Thorsten Brenner ◽  
Thomas H. Fleming ◽  
David Spranz ◽  
Peter Schemmer ◽  
Thomas Bruckner ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Plasma Levels ◽  
Asymmetric Dimethylarginine ◽  
Advanced Glycation Endproducts ◽  
Reactive Metabolites ◽  
Minor Importance ◽  
Advanced Glycation ◽  
Glycation Endproducts ◽  
Reactive Carbonyl Species ◽  
Deceased Donors

Recent investigations have indicated that reactive metabolites and AGE-RAGE-mediated inflammation might play an important role in the pathogenesis of ischemia-reperfusion injury in liver transplantation. In this observational clinical study, 150 patients were enrolled following liver transplantation from deceased donors. The occurrence of short-term complications within 10 days of transplantation was documented. Blood samples were collected prior to transplantation, immediately after transplantation, and at consecutive time points, for a total of seven days after transplantation. Plasma levels of methylglyoxal were determined using HPLC, whereas plasma levels of L-arginine, asymmetric dimethylarginine, advanced glycation endproducts-carboxylmethyllysine, soluble receptor for advanced glycation endproducts, and total antioxidant capacity were measured by ELISA. Patients following liver transplantation were shown to suffer from increased RAGE-associated inflammation with an AGE load mainly dependent upon reactive carbonyl species-derived AGEs. In contrast, carboxylmethyllysine-derived AGEs were of a minor importance. As assessed by the ratio of L-arginine/asymmetric dimethylarginine, the bioavailability of nitric oxide was shown to be reduced in hepatic IRI, especially in those patients suffering from perfusion disorders following liver transplantation. For the early identification of patients at high risk of perfusion disorders, the implementation of asymmetric dimethylarginine measurements in routine diagnostics following liver transplantation from deceased donors should be taken into consideration.

Inhibitory Effects of Lamiaceae Plants on the Formation of Advanced Glycation Endproducts (AGEs) in Model Proteins

2013 ◽  
Vol 2 (4) ◽  
pp. 224-227
Author(s):  
Khwanta Kaewnarin ◽  
Lalida Shank ◽  
Hataichanoke Niamsup ◽  
Nuansri Rakariyatham
Keyword(s):  
Advanced Glycation Endproducts ◽  
Inhibitory Effects ◽  
Advanced Glycation ◽  
Glycation Endproducts

Inhibitory effects of microalgal extracts on the formation of advanced glycation endproducts (AGEs)

2010 ◽  
Vol 120 (1) ◽  
pp. 261-267 ◽  
Author(s):  
Zheng Sun ◽  
Xiaofang Peng ◽  
Jin Liu ◽  
King-Wai Fan ◽  
Mingfu Wang ◽  
...  
Keyword(s):  
Advanced Glycation Endproducts ◽  
Inhibitory Effects ◽  
Advanced Glycation ◽  
Glycation Endproducts

Cinnamon Bark Proanthocyanidins as Reactive Carbonyl Scavengers To Prevent the Formation of Advanced Glycation Endproducts

2008 ◽  
Vol 56 (6) ◽  
pp. 1907-1911 ◽  
Author(s):  
Xiaofang Peng ◽  
Ka-Wing Cheng ◽  
Jinyu Ma ◽  
Bo Chen ◽  
Chi-Tang Ho ◽  
...  
Keyword(s):  
Advanced Glycation Endproducts ◽  
Advanced Glycation ◽  
Cinnamon Bark ◽  
Glycation Endproducts ◽  
Reactive Carbonyl
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