Evaluation of photodynamic therapy efficiency using an in vitro three-dimensional microfluidic breast cancer tissue model

Lab on a Chip ◽  
2015 ◽  
Vol 15 (3) ◽  
pp. 735-744 ◽  
Author(s):  
Yamin Yang ◽  
Xiaochuan Yang ◽  
Jin Zou ◽  
Chao Jia ◽  
Yue Hu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Photodynamic Therapy ◽  
Three Dimensional ◽  
Therapeutic Agents ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Tissue Model

A microfluidic-based in vitro three-dimensional (3D) breast cancer tissue model was established for determining the efficiency of photodynamic therapy (PDT) with therapeutic agents (photosensitizer and gold nanoparticles) under various irradiation conditions.

scholarly journals Patient-derived tumor spheroid cultures as a promising tool to assist personalized therapeutic decisions in breast cancer

2021 ◽  
Author(s):  
Sarah U Hofmann ◽  
Raichel Cohen-Harazi ◽  
Yael Maizels ◽  
Igor Koman
Keyword(s):  
Breast Cancer ◽  
Treatment Plan ◽  
Statistical Significance ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Intrinsic Resistance ◽  
Significant Group ◽  
Chemotherapy Drugs ◽  
Therapeutic Decisions

Breast cancer is the most common cause of cancer related death in women. Treatment of breast cancer has many limitations including a lack of accurate biomarkers to predict success of chemotherapy and intrinsic resistance of a significant group of patients to the gold standard of therapy. Therefore, new tools are needed to provide doctors with guidance in choosing the most effective treatment plan for a particular patient and thus to increase the survival rate for breast cancer patients. Here, we present a successful method to grow in vitro spheroids from primary breast cancer tissue. Samples were received in accordance with relevant ethical guidelines and regulations. After tissue dissociation, in vitro spheroids were generated in a scaffold-free 96-well plate format. Spheroid composition was investigated by immunohistochemistry (IHC) of epithelial (Pan Cytokeratin (panCK)), stromal (Vimentin) and breast cancer-specific markers (ER, PR, HER2, GATA-3). Growth and cell viability of the spheroids were assessed upon treatment with multiple anti-cancer compounds. Students t-test and two-way ANOVA test were used to determine statistical significance. We were able to successfully grow spheroids from 27 out of 31 samples from surgical resections of breast cancer tissue from previously untreated patients. Recapitulation of the histopathology of the tissue of origin was confirmed. Furthermore, a drug panel of standard first- and second-line chemotherapy drugs used to treat breast cancer was applied to assess the viability of the patient-derived spheroids and revealed variation between samples in the response of the spheroids to different drug treatments. We investigated the feasibility and the utility of an in vitro patient-derived spheroid model for breast cancer therapy, and we conclude that spheroids serve as a highly effective platform to explore cancer therapeutics and personalized treatment efficacy. These results have significant implications for the application of this model in clinical personalized medicine.

Estrogen-Receptor-Negative Breast Cancer Tissue Is Chemosensitive in vitro Compared with Estrogen-Receptor-Positive Tissue

1990 ◽  
Vol 22 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Y. Maehara ◽  
Y. Emi ◽  
Y. Sakaguchi ◽  
T. Kusumoto ◽  
Y. Kakeji ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Estrogen Receptor Positive ◽  
Estrogen Receptor Negative

Expression of P-glycoprotein in breast cancer tissue and in vitro resistance to doxorubicin and vincristine

1991 ◽  
Vol 27 (2) ◽  
pp. 155-158 ◽  
Author(s):  
O. Sanfilippo ◽  
E. Ronchi ◽  
C. De Marco ◽  
G. Di Fronzo ◽  
R. Silvestrini
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
P Glycoprotein ◽  
Resistance To Doxorubicin

scholarly journals KIF21A influences breast cancer metastasis and survival

2016 ◽  
Author(s):  
Anton J. Lucanus ◽  
Victoria King ◽  
George W. Yip
Keyword(s):  
Breast Cancer ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
Breast Cancer Tissue ◽  
Clinicopathological Parameters ◽  
Cancer Tissue ◽  
Human Breast ◽  
Mcf 7

ABSTRACTBreast cancer pathogenesis is known to be propagated by the differential expression of a group of proteins called the Kinesin Superfamily (KIFs), which are instrumental in the intracellular transport of chromosomes along microtubules during mitosis. During mitosis, KIFs are strictly regulated through temporal synthesis so that they are only present when needed. However, their misregulation may contribute to uncontrolled cell growth due to premature sister chromatid separation, highlighting their involvement in tumorigenesis. One particular KIF, KIF21A, was recently found to promote the survival of human breast cancer cells in vitro. However, how KIF21A influences other cancerous phenotypes is currently unknown. This study therefore aimed to consolidate the in vitro role of KIF21A in breast cancer metastasis, while also analysing KIF21A expression in human breast cancer tissue to determine its prognostic value. This was achieved by silencing KIF21A in MCF-7 and MDA-MB-231 breast cancer cell lines via siRNA transfection. Migration, invasion, proliferation, and adhesion assays were then performed to measure the effects of KIF21A silencing on oncogenic behaviour. Immunohistochemistry was also conducted in 263 breast cancer tissue samples to compare KIF21A expression levels against various prognostic outcomes and clinicopathological parameters. KIF21A knockdown reduced cell migration (by 42.8% [MCF-7] and 69.7% [MDA-MB-231]) and invasion (by 72.5% [MCF-7] and 42.5% [MDA-MB-231]) in both cell lines, but had no effect on adhesion or proliferation, suggesting that KIF21A plays an important role in the early stages of breast cancer metastasis. Unexpectedly however, KIF21A was shown to negatively correlate with various pro-malignant clinicopathological parameters, including tumour size and histological grade, and high KIF21A expression predicted better breast cancer survival (hazard ratio = 0.45), suggesting that KIF21A is a tumour suppressor. The conflicting outcomes of in vitro and in vivo data may be due to the possible multi-functionality of KIF21A or study limitations, and means no definitive conclusions can be drawn about the role of KIF21A in breast cancer. This warrants further investigation, which may prove pivotal to the development of novel chemotherapeutic strategies to mediate KIF21A’s function and enhance prognostic outcomes.

Determining tamoxifen sensitivity using primary breast cancer tissue in collagen-based three-dimensional culture

Biomaterials ◽  
2012 ◽  
Vol 33 (3) ◽  
pp. 907-915 ◽  
Author(s):  
Alexander D. Leeper ◽  
Joanne Farrell ◽  
Linda J. Williams ◽  
Jeremy S. Thomas ◽  
J. Michael Dixon ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Primary Breast Cancer ◽  
Three Dimensional ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Three Dimensional Culture ◽  
Primary Breast Cancer Tissue ◽  
Tamoxifen Sensitivity

Peroxidatic catecholestrogen production by human breast cancer tissue in vitro

1987 ◽  
Vol 28 (5) ◽  
pp. 513-520 ◽  
Author(s):  
Michael Levin ◽  
Judith Weisz ◽  
Quang D. Bui ◽  
Richard J. Santen
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Human Breast ◽  
Human Breast Cancer Tissue

scholarly journals LncRNA EGFR-AS1 Regulates Breast Cancer Cells Function and Sensitivity to Docetaxel Via the miR-149-5p/ELP5 Axis

2021 ◽  
Author(s):  
Shiping Li ◽  
Xiaoyi Mi ◽  
Mingfang Sun ◽  
Jie Zhang ◽  
Miaomiao Hao ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Western Blotting ◽  
Breast Cancer Cells ◽  
Breast Cancer Tissue ◽  
Luciferase Reporter ◽  
Cancer Tissue ◽  
Cancer Tissues

Abstract Background: Recently, an increasing number of studies have focused on investigating long non-coding RNAs (lncRNAs) and their role in regulating the progression of various cancer types. However, the biological effects and underlying mechanisms of EGFR-AS1, a typical lncRNA, remain largely unclear in breast cancer.Methods: Differential expression of EGFR-AS1 in breast cancer tissue was analyzed using an integrative database and verified in breast cancer tissue samples and cells via real-time PCR analysis and western blotting analysis. The tumor promoter role of EGFR-AS1 in breast cancer cells was determined through MTT, EDU analysis, colony formation and transwell assays,and the effect of EGFR-AS1 on docetaxel drug sensitivity was examined. We then performed bioinformatic analysis and the dual-luciferase reporter assay to identify the binding sites of EGFR-AS1/miR-149-5p and miR-149-5p/ELP5. Results from western blotting and biological function studies provided insights into whether the EGFR-AS1/miR-149-5p/ELP5 axis regulates breast cancer development in vitro and in vivo. Results: EGFR-AS1 is upregulated in breast cancer tissues and cells and promotes the progression of breast cancer cells both in vitro and in vivo. Moreover, miR-149-5p is downregulated in breast cancer tissues and cell lines. Mechanistically, EGFR-AS1 regulates ELP5 levels by sponging miR-149-5p, thereby affecting cell progression and promoting epithelial-to-mesenchymal transition. Hence, the EGFR-AS1/miR-149-5p/ELP5 axis is involved in breast cancer proliferation, migration, invasion, and resistance to the chemotherapeutic drug, docetaxel, in breast cancer cells. Conclusions: EGFR-AS1 sponges miR-149-5p to affect the expression level of ELP5 ultimately acting as a new tumor promotor in breast cancer. This study provides novel insights into diagnostic and docetaxel-related chemotherapy targets for breast cancer.

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