Light up ClO−in live cells using an aza-coumarin based fluorescent probe with fast response and high sensitivity

An aza-coumarin based fluorescent and colorimetricAC-ClOfor the ClO−determination with fast response and high sensitivity.AC-ClOwas successfully applied for the live-cell imaging of exogenous and endogenous ClO−.

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A Michael addition–cyclization-based switch-on fluorescent chemodosimeter for cysteine and its application in live cell imaging

New Journal of Chemistry ◽

10.1039/c8nj00465j ◽

2018 ◽

Vol 42 (7) ◽

pp. 4951-4958 ◽

Cited By ~ 10

Author(s):

Srimanta Manna ◽

Parthasarathi Karmakar ◽

Syed Samim Ali ◽

Uday Narayan Guria ◽

Ripon Sarkar ◽

...

Keyword(s):

Fluorescent Probe ◽

Michael Addition ◽

Live Cell Imaging ◽

Cell Imaging ◽

Fast Response ◽

Live Cell ◽

Fluorescent Chemodosimeter

We designed and synthesized a fast response fluorescent probe, BTAC (benzothiazol-azacoumarin), for detection of cysteine (Cys).

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A hydrazone dual-functional fluorescent probe based on carbazole and coumarin groups for the detection of Cu2+ and ClO−: Application in live cell imaging and actual water samples

Journal of Photochemistry and Photobiology A Chemistry ◽

10.1016/j.jphotochem.2021.113593 ◽

2021 ◽

pp. 113593

Author(s):

Li Liu ◽

Changqing Guo ◽

Qisheng Zhang ◽

Peipei Xu ◽

Yuanyuan Cui ◽

...

Keyword(s):

Fluorescent Probe ◽

Water Samples ◽

Live Cell Imaging ◽

Cell Imaging ◽

Live Cell ◽

Dual Functional

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tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

10.1101/2021.04.27.441613 ◽

2021 ◽

Author(s):

Y. Bousmah ◽

H. Valenta ◽

G. Bertolin ◽

U. Singh ◽

V. Nicolas ◽

...

Keyword(s):

Single Molecule ◽

Live Cell Imaging ◽

Cell Imaging ◽

Fluorescent Protein ◽

Fluorescent Proteins ◽

Yellow Fluorescent Protein ◽

Resonance Energy ◽

Live Cell ◽

Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

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CRISPR-mediated Multiplexed Live Cell Imaging of Nonrepetitive Genomic Loci

10.1101/2020.03.03.974923 ◽

2020 ◽

Author(s):

Patricia A. Clow ◽

Nathaniel Jillette ◽

Jacqueline J. Zhu ◽

Albert W. Cheng

Keyword(s):

Live Cell Imaging ◽

Cell Imaging ◽

Repetitive Sequences ◽

Binary Sequence ◽

Three Dimensional ◽

Live Cell ◽

Live Cells ◽

Imaging Method ◽

Genomic Locus ◽

Time Dynamics

AbstractThree-dimensional (3D) structures of the genome are dynamic, heterogeneous and functionally important. Live cell imaging has become the leading method for chromatin dynamics tracking. However, existing CRISPR- and TALE-based genomic labeling techniques have been hampered by laborious protocols and low signal-to-noise ratios (SNRs), and are thus mostly applicable to repetitive sequences. Here, we report a versatile CRISPR/Casilio-based imaging method, with an enhanced SNR, that allows for one nonrepetitive genomic locus to be labeled using a single sgRNA. We constructed Casilio dual-color probes to visualize the dynamic interactions of cohesin-bound elements in single live cells. By forming a binary sequence of multiple Casilio probes (PISCES) across a continuous stretch of DNA, we track the dynamic 3D folding of a 74kb genomic region over time. This method offers unprecedented resolution and scalability for delineating the dynamic 4D nucleome.One Sentence SummaryCasilio enables multiplexed live cell imaging of nonrepetitive DNA loci for illuminating the real-time dynamics of genome structures.

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