Rapid screening of antibody–antigen binding using dynamic light scattering (DLS) and gold nanoparticles

2015 ◽  
Vol 7 (17) ◽  
pp. 7249-7255 ◽  
Author(s):  
Yen H. Lai ◽  
Sunghyu Koo ◽  
Soon Hwan Oh ◽  
Elizabeth A. Driskell ◽  
Jeremy D. Driskell
Keyword(s):  
Gold Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Gold Nanoparticle ◽  
Rapid Screening ◽  
Antigen Binding

Antigen-mediated aggregation of antibody–gold nanoparticle conjugates is detected with dynamic light scattering to evaluate antibody–antigen binding.

scholarly journals A simple and sensitive sensor for silver ions based on unmodified gold nanoparticles by using dynamic light scattering techniques

2017 ◽  
Vol 95 (12) ◽  
pp. 1267-1272 ◽  
Author(s):  
Zhiyou Xiao ◽  
Anjiang Tang ◽  
Hongsheng Huang ◽  
Ze Wang
Keyword(s):  
Gold Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
High Sensitivity ◽  
Silver Ions ◽  
Hydrodynamic Diameter ◽  
Optimum Conditions ◽  
Sensitivity And Selectivity ◽  
Sensitive Sensor ◽  
Average Hydrodynamic Diameter

A simple and sensitive assay for Ag+ was developed with unmodified gold nanoparticles (AuNPs) by using dynamic light scattering techniques. Ag+ could induce the oligonucleotide (5′-ATC ACT ATA TCA TAT ACT CAT-3′) to change from a single-stranded structure to a double-stranded structure and desorb from the surface of AuNPs, which triggered the aggregation of AuNPs in the salt solution. The average hydrodynamic diameter of aggregated AuNPs could be detected by using dynamic light scattering techniques. Under the optimum conditions, the average hydrodynamic diameter of AuNPs is proportional to the concentration of Ag+ within the range of 13.3–100.0 nmol/L, with a detection limit of 3.2 nmol/L. The method is easy to operate and has low sample consumption, high sensitivity and selectivity.

Diffusion of Gold Nanoparticles in Inverse Opals Probed by Heterodyne Dynamic Light Scattering

2019 ◽  
Vol 131 (2) ◽  
pp. 723-737 ◽  
Author(s):  
Cédric Giraudet ◽  
Matthias S. G. Knoll ◽  
Yaraset Galvan ◽  
Sebastian Süß ◽  
Doris Segets ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Inverse Opals

scholarly journals Acetylcholine and acetylcholinesterase inhibitors detection using gold nanoparticles coupled with dynamic light scattering

2020 ◽  
Vol 1 ◽  
pp. 100007
Author(s):  
Amal El Alami ◽  
Fabienne Lagarde ◽  
Qun Huo ◽  
Tianyu Zheng ◽  
Mimouna Baitoul ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Acetylcholinesterase Inhibitors

Dynamic Light Scattering as a Powerful Tool for Gold Nanoparticle Bioconjugation and Biomolecular Binding Studies

2009 ◽  
Vol 81 (22) ◽  
pp. 9425-9432 ◽  
Author(s):  
Hilde Jans ◽  
Xiong Liu ◽  
Lauren Austin ◽  
Guido Maes ◽  
Qun Huo
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Gold Nanoparticle ◽  
Binding Studies ◽  
Biomolecular Binding
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