scholarly journals Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow

Lab on a Chip ◽  
2015 ◽  
Vol 15 (10) ◽  
pp. 2269-2277 ◽  
Author(s):  
Mandy B. Esch ◽  
Jean-Matthieu Prot ◽  
Ying I. Wang ◽  
Paula Miller ◽  
Jose Ricardo Llamas-Vidales ◽  
...  
Keyword(s):  
Cell Culture ◽  
Metabolic Activity ◽  
Kupffer Cells ◽  
Liver Cell ◽  
Low Cost ◽  
Stellate Cells ◽  
Cell Types ◽  
Liver Cell Culture ◽  
Parenchymal Cells

We have developed a low-cost liver cell culture device that creates fluidic flow over a 3D primary liver cell culture that consists of multiple liver cell types, including hepatocytes and non-parenchymal cells (fibroblasts, stellate cells, and Kupffer cells).

scholarly journals The uptake and metabolism of chylomicron-remnant lipids by rat liver parenchymal and non-parenchymal cells in vitro

1985 ◽  
Vol 232 (2) ◽  
pp. 395-401 ◽  
Author(s):  
P M Lippiello ◽  
P J Sisson ◽  
M Waite
Keyword(s):  
Endothelial Cells ◽  
Rat Liver ◽  
Kupffer Cells ◽  
Liver Cell ◽  
Cholesterol Ester ◽  
Cell Types ◽  
Chylomicron Remnant ◽  
Parenchymal Cells ◽  
Uptake And Metabolism

The uptake and metabolism of chylomicron-remnant lipids by individual liver cell types was examined by incubating remnants with monolayer cultures of hepatocytes, Kupffer cells, and endothelial cells from rat liver. Remnants were prepared in vitro from radiolabelled mesenteric-lymph chylomicra, utilizing either purified lipoprotein lipase from bovine milk, or plasma isolated from heparinized rats. The resulting particles contained [3H]phosphatidylcholine and cholesterol, and [14C]oleate in the acylglycerol, phospholipid, fatty-acid and cholesterol-ester fractions. The capacities of the three cell types for uptake of both [3H]lipids and [14C]lipids were determined to be, on a per-cell basis, in the order: Kupffer greater than hepatocytes greater than endothelial. The relative proportions of [3H]phospholipid and total [3H]cholesterol taken up by hepatocytes and non-parenchymal cells remained constant with time. The uptake of [14C]oleoyl lipids by all three cell types was slightly greater than that of the total [3H]cholesterol and [3H]phospholipid components. There was evidence of cholesterol-ester hydrolysis and turnover of [14C]oleate in the phospholipid fraction in hepatocytes and Kupffer cells, but not endothelial cells, over the first 2 h. With both remnant preparations, these observations indicate that significant differences exist between the three major liver cell types with respect to the uptake and metabolism of remnant lipid components.

Accessibility of Primary Bile in Liver Cell Culture

1989 ◽  
pp. 87-88
Author(s):  
F. Wehner ◽  
E. Petzinger ◽  
H. Acker ◽  
J. Hentschel ◽  
R. K. H. Kinne
Keyword(s):  
Cell Culture ◽  
Liver Cell ◽  
Liver Cell Culture

Diffusive and convective transport through hollow fiber membranes for liver cell culture

2005 ◽  
Vol 117 (3) ◽  
pp. 309-321 ◽  
Author(s):  
E. Curcio ◽  
L. De Bartolo ◽  
G. Barbieri ◽  
M. Rende ◽  
L. Giorno ◽  
...  
Keyword(s):  
Cell Culture ◽  
Liver Cell ◽  
Hollow Fiber ◽  
Convective Transport ◽  
Hollow Fiber Membranes ◽  
Liver Cell Culture ◽  
Fiber Membranes

Liver cell culture toxicity of general anesthetics

1976 ◽  
Vol 36 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Yoshiaki Goto ◽  
Carlos A. Dujovne ◽  
Don W. Shoeman ◽  
Kasumi Arakawa
Keyword(s):  
Cell Culture ◽  
Liver Cell ◽  
General Anesthetics ◽  
Liver Cell Culture
Sign in / Sign up

Export Citation Format

Share Document