scholarly journals Harmonized tuning of nucleic acid and lectin binding properties with multivalent cyclodextrins for macrophage-selective gene delivery

RSC Advances ◽  
2015 ◽  
Vol 5 (93) ◽  
pp. 76464-76471 ◽  
Author(s):  
Alejandro Méndez-Ardoy ◽  
Alejandro Díaz-Moscoso ◽  
Carmen Ortiz Mellet ◽  
Christophe Di Giorgio ◽  
Pierre Vierling ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gene Delivery ◽  
Cell Lines ◽  
Lectin Binding ◽  
Viral Gene ◽  
Binding Properties ◽  
Gene Carriers

Polycationic amphiphilic cyclodextrins (paCDs) have been shown to behave as efficient non-viral gene carriers paralleling the efficacy of commercial vectors towards a variety of cell lines.

Newly established human pancreatic carcinoma cell lines and their lectin binding properties

1993 ◽  
Vol 13 (1) ◽  
pp. 31-41
Author(s):  
Nobuyuki Nishimura ◽  
Seiji Saito ◽  
Yoshiki Kubota ◽  
Nan-yo Moto-o ◽  
Kuniko Taguchi ◽  
...  
Keyword(s):  
Pancreatic Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Lectin Binding ◽  
Binding Properties ◽  
Carcinoma Cell Lines ◽  
Human Pancreatic Carcinoma Cell

scholarly journals Estimating the Two Graph Dextran-Stearic Acid-Spermine Polymers Based on Iron Oxide Nanoparticles as Carrier for Gene Delivery

2021 ◽  
Author(s):  
Mehrnoosh Kazemi Ashtiyani ◽  
Behnam Hajipour-Verdom ◽  
Mohammad Satari ◽  
Parviz Abdolmaleki ◽  
Saman Hosseinkhani
Keyword(s):  
Gene Delivery ◽  
Stearic Acid ◽  
Electrostatic Interactions ◽  
Critical Role ◽  
Amphiphilic Polymer ◽  
Superparamagnetic Nanoparticles ◽  
Viral Gene ◽  
Surface Charges ◽  
Magnetic Carriers ◽  
Gene Carriers

Non-viral gene carriers because of their limited side effects, biocompatibility, simplicity and taking the advantages of electrostatic interactions have shown noticeable potential in gene delivery. The low transfection rate of non-viral vectors under physiological conditions is a significant issue. Here, the aim of this study was to investigate the efficacy of hydrophilic and hydrophobic groups on gene carriers such as two synthesized amphiphilic polymer of dextran-stearic acid-spermine (DSASP) with verified lipid and amine conjugations that associated with Fe3O4 superparamagnetic nanoparticles to promote the target delivery and decrease the transfection time using static magnetic field. Our findings illustrate that magnetic nanoparticles are spherical with positive surface charges and superparamagnetic behaviors. The DSASP–pDNA/MNPs offered a strong pDNA condensation, protection against DNase degradation, significant cell viability in HEK 293T cells and. Although conjugations of spermine play a critical role in transfection efficiency, amphiphilic polymer with more derivatives of stearic acid showed better transfection yields. Therefore, DSASP amphiphilic magnetic carriers offer new insights for gene delivery due to the amine contents and ameliorate the uptake of complexes via cell membrane based on its hydrophilic surface.

scholarly journals A Triple-Fluorophore-Labeled Nucleic Acid pH Nanosensor to Investigate Non-viral Gene Delivery

2017 ◽  
Vol 25 (7) ◽  
pp. 1697-1709 ◽  
Author(s):  
David R. Wilson ◽  
Denis Routkevitch ◽  
Yuan Rui ◽  
Arman Mosenia ◽  
Karl J. Wahlin ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gene Delivery ◽  
Viral Gene ◽  
Acid Ph ◽  
Viral Gene Delivery

Stability of lectin binding properties expressed by human bladder carcinoma cell lines passaged in vitro or in nude mice

1988 ◽  
Vol 16 (6) ◽  
pp. 407-414 ◽  
Author(s):  
P. J. Russell ◽  
J. Wotherspoon ◽  
M. Jelbart ◽  
J. Philips ◽  
D. Raghavan
Keyword(s):  
Cell Lines ◽  
Nude Mice ◽  
Bladder Carcinoma ◽  
Carcinoma Cell ◽  
Lectin Binding ◽  
Human Bladder ◽  
Binding Properties ◽  
Carcinoma Cell Lines

Zn-promoted gene transfection efficiency for non-viral vectors: a mechanism study

2021 ◽  
Author(s):  
Xiao-Qi Yu ◽  
Rui-Mo Zhao ◽  
Yu Guo ◽  
Hui-Zhen Yang ◽  
Ji Zhang
Keyword(s):  
Gene Therapy ◽  
Nucleic Acid ◽  
Gene Delivery ◽  
Viral Vectors ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Viral Gene ◽  
Mechanism Study ◽  
High Affinity ◽  
Gene Vectors

The development of cationic non-viral gene vectors that may overcome the obstacles in gene delivery is of great significance to gene therapy. Metallic complexes with high affinity to nucleic acid...

scholarly journals Estimating the Two Graph Dextran-Stearic Acid-Spermine Polymers Based on Iron Oxide Nanoparticles as Carrier for Gene Delivery

2021 ◽  
Author(s):  
Mehrnoosh Kazemi Ashtiyani ◽  
Behnam Hajipour-Verdom ◽  
Mohammad Satari ◽  
Parviz Abdolmaleki ◽  
Saman Hosseinkhani ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Stearic Acid ◽  
Electrostatic Interactions ◽  
Critical Role ◽  
Amphiphilic Polymer ◽  
Superparamagnetic Nanoparticles ◽  
Viral Gene ◽  
Surface Charges ◽  
Magnetic Carriers ◽  
Gene Carriers

Abstract BackgroundNon-viral gene carriers because of their limited side effects, biocompatibility, simplicity and taking the advantages of electrostatic interactions have shown noticeable potential in gene delivery. The low transfection rate of non-viral vectors under physiological conditions is a significant issue. MethodsWe investigated the efficacy of hydrophilic and hydrophobic groups on gene carriers such as two synthesized amphiphilic polymer of dextran-stearic acid-spermine (DSASP) with verified lipid and amine conjugations that associated with Fe3O4 superparamagnetic nanoparticles to promote the target delivery and decrease the transfection time using static magnetic field.ResultsOur findings illustrate that magnetic nanoparticles are spherical with positive surface charges and superparamagnetic behaviors. The DSASP–pDNA/MNPs offered a strong pDNA condensation, protection against DNase degradation, significant cell viability in HEK 293T cells. Although conjugations of spermine play a critical role in transfection efficiency, amphiphilic polymer with more derivatives of stearic acid showed better transfection yields. ConclusionDSASP amphiphilic magnetic carriers offer new insights for gene delivery due to the amine contents and ameliorate the uptake of complexes via cell membrane based on its hydrophilic surface.

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