scholarly journals Glutamine-chitosan modified calcium phosphate nanoparticles for efficient siRNA delivery and osteogenic differentiation

2015 ◽  
Vol 3 (31) ◽  
pp. 6448-6455 ◽  
Author(s):  
Bogyu Choi ◽  
Zhong-Kai Cui ◽  
Soyon Kim ◽  
Jiabing Fan ◽  
Benjamin M. Wu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Gene Transfection ◽  
Sirna Delivery ◽  
Calcium Phosphate Nanoparticles

CaP nanoparticles coated with highly cationic, glutamine-conjugated oligochitosan (Gln-OChi) are developed for siRNA delivery to significantly enhance gene transfection and knockdown efficiency with minimal cytotoxicity. This new nanocarrier can potentially be used for gene therapy.

Polycation liposomes combined with calcium phosphate nanoparticles as a non-viral carrier for siRNA delivery

2015 ◽  
Vol 30 ◽  
pp. 1-6 ◽  
Author(s):  
Jianjun Zhang ◽  
Xiaoyi Sun ◽  
Rong Shao ◽  
Wenquan Liang ◽  
Jianqing Gao ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Sirna Delivery ◽  
Calcium Phosphate Nanoparticles

Gene transfection achieved by utilizing antibacterial calcium phosphate nanoparticles for enhanced regenerative therapy

2021 ◽  
Vol 119 ◽  
pp. 375-389
Author(s):  
Chen Xiang ◽  
Taichi Tenkumo ◽  
Toru Ogawa ◽  
Yoshiaki Kanda ◽  
Keisuke Nakamura ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Gene Transfection ◽  
Regenerative Therapy ◽  
Calcium Phosphate Nanoparticles

scholarly journals Gene transfection of human mesenchymal stem cells with a nano-hydroxyapatite-collagen scaffold containing DNA-functionalized calcium phosphate nanoparticles

2016 ◽  
Vol 21 (7) ◽  
pp. 682-695 ◽  
Author(s):  
Taichi Tenkumo ◽  
Juan Ramón Vanegas Sáenz ◽  
Yukyo Takada ◽  
Masatoshi Takahashi ◽  
Olga Rotan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Gene Transfection ◽  
Human Mesenchymal Stem Cells ◽  
Collagen Scaffold ◽  
Calcium Phosphate Nanoparticles

Calcium Phosphate Nanoparticles as a Novel Nonviral Vector for Efficient Transfection of DNA in Cancer Gene Therapy

2005 ◽  
Vol 20 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Ting Liu ◽  
Aifa Tang ◽  
Guiying Zhang ◽  
Yuxiang Chen ◽  
Junyi Zhang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Cancer Gene Therapy ◽  
Cancer Gene ◽  
Nonviral Vector ◽  
Calcium Phosphate Nanoparticles

PREPARATION OF CALCIUM PHOSPHATE NANOPARTICLES AS NON-VIRAL VECTORS FOR GENE DELIVERY SYSTEM

2017 ◽  
Vol 29 (04) ◽  
pp. 1750027 ◽  
Author(s):  
Ko-Chung Yen ◽  
I-Hua Chen ◽  
Feng-Huei Lin
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Vectors ◽  
Fluorescent Protein ◽  
Molar Ratio ◽  
Gene Delivery System ◽  
Calcium Phosphate Nanoparticles

A major aim of gene therapy is the efficient and specific delivery of therapeutic gene into the desired target tissues. Development of reliable vectors is a major challenge in gene therapy. The aim of this study is to develop calcium phosphate nanoparticles as novel non-viral vectors for the gene delivery system. Calcium phosphate nanoparticles were prepared by water-in-oil microemulsion method with a water to surfactant molar ratio, Wo [Formula: see text] 2–10. This paper studies the design and synthesis of ultra-low size, highly monodispersed DNA doped calcium phosphate nanoparticles of size around 100[Formula: see text]nm in diameter. The structure of DNA-calcium phosphate nanocomplex observed by TEM was displayed as a shell-like structure. This study used pEGFP as a reporter gene. The encapsulating efficiency to encapsulate DNA inside the nanoparticles was greater than 80%. In the MTT test, both calcium phosphate nanoparticles and DNA-calcium phosphate nanocomplex have no negative effect for 293T cells. By gel electrophoresis of free and entrapped pEGFP DNA, the DNA encapsulated inside the nanoparticles was protected from the external DNaseI environment. In vitro transfection studies in 293T cell-line, the DNA-calcium phosphate nanocomplex could be used safely to transfer the encapsulated DNA into the 293T cells and expression green fluorescent protein. The characteristic of DNA-calcium phosphate nanocomplex to deliver DNA belongs to slow release. The property of DNA-calcium phosphate nanocomplex was fit in the requirement of non-viral vectors for the gene delivery system.

scholarly journals Incorporation of well-dispersed calcium phosphate nanoparticles into PLGA electrospun nanofibers to enhance the osteogenic induction potential

RSC Advances ◽  
2017 ◽  
Vol 7 (39) ◽  
pp. 23982-23993 ◽  
Author(s):  
Dandan Wang ◽  
Liuyang Xuan ◽  
Huixiang Zhong ◽  
Yihong Gong ◽  
Xuetao Shi ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Electrospun Nanofibers ◽  
Calcium Phosphate Nanoparticles ◽  
Osteogenic Induction ◽  
Zn Nanoparticles

PAA modified Zn-doped HAp-like calcium phosphate (PAA-CaP/Zn) nanoparticles were homogeneously distributed in PLGA electrospun nanofibers, and enhanced the osteogenic differentiation of rADSCs.

scholarly journals Amiloride-enhanced gene transfection of octa-arginine functionalized calcium phosphate nanoparticles

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0188347 ◽  
Author(s):  
Juan Ramón Vanegas Sáenz ◽  
Taichi Tenkumo ◽  
Yuya Kamano ◽  
Hiroshi Egusa ◽  
Keiichi Sasaki
Keyword(s):  
Calcium Phosphate ◽  
Gene Transfection ◽  
Calcium Phosphate Nanoparticles
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